203 results about "Gene probe" patented technology

The subject invention is a series of methods and systems for using the Surface-Enhanced Raman (SER)-labeled Gene Probe for hybridization, detection and identification of SER-labeled hybridized target oligonucleotide material comprising the steps of immobilizing SER-labeled hybridized target oligonucleotide material on a support means, wherein the SER-labeled hybridized target oligonucleotide material comprise a SER label attached either to a target oligonucleotide of unknown sequence or to a gene probe of known sequence complementary to the target oligonucleotide sequence, the SER label is unique for the target oligonucleotide strands of a particular sequence wherein the SER-labeled oligonucleotide is hybridized to its complementary oligonucleotide strand, then the support means having the SER-labeled hybridized target oligonucleotide material adsorbed thereon is SERS activated with a SERS activating means, then the support means is analyzed.

The invention generally relates to a new gene probe biosensor employing near field surface enhanced Raman scattering (NFSERS) for direct spectroscopic detection of hybridized molecules (such as hybridized DNA) without the need for labels, and the invention also relates to methods for using the biosensor.

The invention relates to a gene chip for high-flux detection of pathogens and application thereof. The gene comprises (1) a combination of 174 oligonucleotide probes of pathogen variety specific genes, toxin genes and drug-resistant genes; and (2) a probe array, which is formed by curing the oligonucleotide probes on a carrier material by arm molecules. The gene chip comprises 174 gene probes, namely 32 pathogen variety specific gene probes of the following 8 pathogens of Burkholderia mallei, Burkholderia pseudomallei, Brucella, salmonella, Yersinia pestis, Bacillus anthracis, comma bacillus and the like, 25 toxin gene probe of the following 7 toxins of diphtheria toxin, Shiga toxin, staphylococcus enterotoxin, choleratoxin and the like, and 117 drug-resistant gene probes of 17 drug-resistant genes of extended-spectrum beta-lactamase, cephalosporinase, carbapenemase, integrase gene, common gene engineering carrier drug-resistant gene and the like. The gene chip can be used to detect multiple pathogen variety specific genes, toxin genes and drug-resistant genes.

The invention relates to a preparation method for probes related to breast cancer molecular markers and to preparation of a breast cancer fluorescence in situ hybridization detection kit by using the probes. The breast cancer fluorescence in situ hybridization detection kit can be prepared from HER2, TOP2A and AGTR1 gene probes prepared by using the method provided in the invention, human chromosome 17 counting probe, hybridization buffer, unlabelled competitive DNA and DAPI counter strain; application of the kit enables the detection rate of breast cancers to be substantially improved and type sorting of breast cancers to be more accurate and provides guidance to formulation of an individual therapeutic schedule and selection of proper therapeutic drugs, thereby lowering down mortality, reducing recurrence risk and achieving the goal of optimizing the effect of diagnosis and treatment.

The invention relates to a gene chip used for detecting gene mutation relating to a high blood pressure personalized medicine. The gene chip for detecting the gene mutation relating to high blood pressure personalized medicine comprises a solid phase support, a gene probe (an oligonueleotide probe) fixed in the solid phase support sequentially and a PCR primer used for amplifying the mutated gene fragment in the sample; the gene probe (the oligonueleotide probe) and the PCR primer are designed aiming at one or two points of the gene mutation in ACE (I / D) and CYP3A5*3 and / or two or more points as follows: CYP2C9*3, CYP2C9*13, AGTR1(A1166C), CYP2D6*10, ADRB1(C1165G), TSC(C1784T), ADRB3(T727C), SCNN1G_rs5729, SCNN1G_rs5723, ENOSA_rs1799983 and GNB3(C825T). The invention provides the gene chip and applications thereof for conveniently, quickly and systematically detecting the gene mutation relating to high blood pressure personalized medicine so as to determine drug reactions.

The invention provides a high accuracy detection method of human papilloma virus genotype. Double probe, which comprises a carcinogenicity early transcription region E6 or E7 gene region and a capsid protein late transcription region L1 or L2 gene region, is adopted to detect every genotype. According to the method, pollution exclusion of PCR amplification product is taken as a premise, identical genotype recognition by double gene probe is taken as a basis, and high sensitivity PCR-mass spectrum combination is taken as a platform. The invention provides a method for high accuracy detection and / or identification of human papilloma virus (HPV) genotype.

The present invention relates to a high-throughput detection chip for drug resistance gene of bacteria, and an application thereof. The detection chip comprises 117 gene probes, the drug resistance gene probes are selected from 17 categories of drug resistance genes, which comprise extended spectrum beta-lactamase, cephalosporinase, carbapenemase, integrase gene, tetracycline resistance gene, aminoglycoside resistance gene, disinfectant resistance gene, erythromycin resistance gene, macrolide efflux gene, vancomycin resistance gene, multidrug resistance efflux pump gene, mupirocin resistance gene, sulfanilamide resistance gene, tylosin resistance gene, fluoroquinolone resistance gene, chloramphenicol acetyltransferase and commonly-used genetic engineering vector resistance gene. The chip is adopted for detecting the resistance gene of the pathogenic bacteria. The chip is characterized in that: the chip comprises (1) 117-oligonucleotide probe composition and quality control probes of 17 categories of the drug resistance genes; (2) probe arrays, wherein the oligonucleotide probes are solidified on the vector material through arm molecules to form the probe arrays.

The present invention relates to a gene chip for detecting several infections lideases and its application. It can be used for detecting, typing and strain-identifying 7 main infections diseases of epidemic hemorrhagic fever, tsutsugamushi disease, leptospirosis, schistosomiasis, malaria, cholera and hemorrhagic enteritis due to 0157:H7. Said invention can select specific gene probe and PCR primer respectively from S gene of epidemic hemorrhagic fever virus, 56KD protein gene of oriental rickettsia, 23 SRNA gene of leptospirosis, 5D antigen gene of schistosomiasis, SSrRNA gene of malarial parasite, 0157 antigen gene of colibacillus 0157:H7, H7 antigen gene and toxic gene and outer membrane OWP protein gene of cholera vibrio and toxic gene.

The present invention relates to a detection chip for performing gene detection to various vibrio and its detection and applications. The invention provides 16S rRNA sequences corresponding to each vibrio of vibrio anguillarum, vibrio harveyi, vibrio alginolyticus, vibrio parahaemolyticus, brilliant vibrio and Fisher vibrio; heat shock protein hsp60 probe sequence; virulence gene probe sequence; 16S rRNA forward primer sequence; 16S rRNA reverse primer sequence; heat shock protein hsp60 forward primer sequence; heat shock protein hsp60 reverse primer sequence; virulence gene forward primer sequence and virulence gene reverse primer sequence. The present invention has specific, sensitive and high-throughput features, can simultaneously detect six kinds of bacteria virulence genes, and the invention will effectively guide the production as an important disease early-warning detection method used in clinical diagnosis of aquatic animals.

The present invention discloses one kind of electrochemical sensor and its preparation process and use. The electrochemical sensor is prepared through decorating the surface of glass-carbon electrode with open-mouthed multi-wall carbon nanotube possessing carboxylated end, fixing BCR / ABL b3-a2 type gene probe through covalent decoration onto the end carboxyl radical of carbon nanotube, hybridizing the probe DNA and the target DNA and adopting curcumin as hybridization indicator for researching the recognizing capacity of single strand fixed on the decorated electrode on complementary DNA. The carbon nanotube has great specific surface area favoring the fixing of DNA on the electrode and excellent electron transferring characteristic for high detection sensitivity of sensor. The sensor is used in detecting chronic granulocytic leukemia gene and has high selectivity and high sensitivity.

The present invention relates to kind of gene test technology, and is especially nanometer particle mark gene probe and its preparation and application. The nanometer particle mark gene probe is usedto replace traditional mark probe for practical application. The preparation of the nanometer particle mark gene probe incldues: the preparation of colloid gold or nanometer particle; the design, synthesis and modification of DNA probe molecule; and the self-assembling synthesis and enclosing of gold mark probe. The probe is suitable for use in various gene hybrid test, nanometer amplification and visual color development, and is especially suitable for application in low integration level diagnosis gene chip.

A nanopartide and its avidin probe are disclosed. Said gene probe for detecting the mutation or non-mutation of DNA is prepared from nanoparticles or colloidal particles and avidin, streptavidin, or antibiotin through preparing colloidal gold and preparing the compound. Its advantges are high sensitivity, specificity and speed.

The present invention relates to a gene probe composition and a kit for acute lymphocytic leukemia detection. The gene probe composition comprises a TEL gene probe, an AML1 gene probe, a PAX5 gene probe, a P16 gene probe and an IKZF1 gene probe. The present invention further provides an acute lymphocytic leukemia fluorescence in situ hybridization detection kit, wherein the kit comprises the gene probe composition. With the kit, concurrent five gene detection can be performed on the same sample even the single leukemia cell, and detection capability and efficiency are significantly improved.

A gene chip for detecting the pathogenic bacteria from blood features that the specific probes, derivative probes, complementary probes, and universal probes are arranged on the nylon film to detect different pathogenic bacteria. Its advantages are high speed and high correctness.

The invention of molecular labeling method of Pyricularia grisea avirulent gene belongs to the field of agricultural biotechnology. The molecular labeling technology includes performing molecular labeling to avirulent gene though assessment from two parental strains and filial generation groups of Pyricularia grisea. M355-356 labeled by SSR near NO. 1 chromosome telomere links with avirulent gene Avr-Pit with a genetic distance of 2.3cm; S487 labeled by RAPD located near NO. 7 chromosome telomere links with avirulent gene Avr-Pia with a genetic distance of 3.5cm; S361 labeled by m677-678 and RAPD located at middle of N0. 3 chromosome telomere links with Pyricularia grisea specified avirulent gene PRE1 with genetic distances of 5.9cm and 6.4. The invention can not only make use of the nontoxic gene marker as gene probe, but also set foundations for further clone of the gene through physical mapping.

The invention provides a gene probe for identifying ingredients of sheep origin and goat origin, which comprises a pair of designed gene probes, that is a sheep origin probe 5'-CTGTAACCCACATTTGCCGAGACG-3'bp24 and a goat origin probe 5'-TGATGAGATGTTTGATGGGG-3'bp20; the probes are positioned on a cytochrome gene b, that is Cytb; used primers are 5'-AAAAAGCTTCCATCCAACATCTCAGCATGATGAAA-3' and 5'-AAACTGCAGCCCCTCAGAATGATATTTGTCCTCA-3'; the amplified fragment is 530-550 bp. Through the adoption of a phenol- chloroform method or a kit method, the DNA of a to-be-tested sample is extracted, PCR amplification is implemented, a point system chip is designed, and hybridization, cleaning and the like are carried out.

The invention discloses a quantitative RT-PCR primer and probe and agent box of PML-RARa fusing gene mRNA fluorescence with BCR-ABL fusing gene primer and probe sequence as SEQ ID NO1-4 and internal reference gene primer and probe sequence as SEQ ID NO5-7, wherein the agent box contains cell cracking liquid, water, RT-PCR reacting liquid, internal reference G6PDHRT-PCR reacting liquid, BCR-ABL fusing gene detecting probe, G6PDH internal reference gene testing probe, composite enzyme, standard material and comparing material; the invention measures the expressive level of L-pattern, S-pattern and V-pattern mRNA simultaneously, rapidly and precisely, which improves inducing buffer rate and lessens early bleeding death.

The invention relates to an ASPL-TFE3 fused renal carcinoma gene probe as well as an application of a kit thereof. Cloned fragments selected by the gene probe are respectively a combination of RP11-634L10, RP11-51H16 and RP11-475F12 and a combination of CTD-2311N12, RP11-416B14, CTD-2522M13, CTD-2516D6, CTD-2312C1, CTD-2248C21 and RP11-959H17. By using the ASPL-TFE3 fused renal carcinoma gene probe, the defects of complexity, time consumption and clinic application unsuitability of RT-PCR and cell karyotyping methods applied in the past are overcome. According to the ASPL-TFE3 fused renal carcinoma gene probe, a unique ASPL-TFE3 fused gene in ASPL-TFE3 fused renal carcinoma is detected by using an FISH technology, so that the renal carcinoma is diagnosed; the gene probe is high in accuracy rate, specificity and success rate, strong in fluorescence signal, simple and rapid in operation and rapid in diagnosis when applied and can be applied to paraffin section; and due to the application of the ASPL-TFE3 fused renal carcinoma gene probe, the specimen detecting range is widened, a novel method for accurately, reliably, simply and conveniently diagnosing the ASPL-TFE3 fused renal carcinoma is established, and the precedent that the ASPL-TFE3 fused renal carcinoma is detected by virtue of FISH is created.

Gene probes for specific regions of chromosome 3 (3p21.3) and chromosome 10 (10q22) have been found to be tools for the diagnosis and prognosis of smoking related cancers such as non-small cell lung cancer (NSCLC). For example, these probes can be used with fluorescence in situ hybridization (FISH), and used to stratify smokers into high and low risk groups, as well as determine a patients susceptibility to the development of smoking related cancers.

The invention discloses a bacteria detecting technology which comprises designing special probes with biological information, including designing 34 pieces oligonucleotide as filtered probes and the corresponding PCR and hybridizing reacting conditions; enlarging via PCR and marking bacteria 23srRNA gene internal sub array with Digoxin, and hybridizing the PCR products and a group of special oligonucleotide probes; after enzyme-linked immunoassay color-rendering, identifying whether has aquatic infectious pathogeny bacteria from the sample.

Disclosed are nucleic acid and amino acid sequences encoded by a novel, prostate specific gene (UC41) and diagnostic techniques for the detection of human prostate cancer utilizing such nucleic acid and amino acid sequences. Genetic probes and methods useful in monitoring the progression and diagnosis of prostate cancer are described. Methods of treatment for prostate cancer utilizing antisense constructs or antibodies specific for UC41 gene products are also described.

The invention relates to the technical field of molecular biology, and discloses a primer, a probe, a method and a kit for detecting a KPC (Klebsiella Pneumoniae Carbapenemases) antibiotic gene. Primers and fluorescent labeled probes are respectively designed aiming at a conservative region of a phoE gene, a KPC gene and an interior standard (int) gene by adopting a Taqman probe real-time fluorescence PCR (Polymerase Chain Reaction) method; the 5' ends of phoE gene probes and KPC gene probes are all labeled by a fluorescent report group FAM, and 3' ends are labeled by a fluorescent quenching group TAMRA; the 5' ends of int genes are labeled by a fluorescent report group JOE, and the 3' ends are labeled by a fluorescent quenching group BHQ1. After the primer and the probe are prepared into a PCR detection mixed solution, enzyme and sample nucleic acid are added, an FAM channel on a fluorescent PCR instrument is selected to amplify the phoE gene and the KPC gene, a JOE channel is selected to amplify the int gene, and detection on a target gene is realized through change of a fluorescent signal. The primer, the probe, the method and the kit, disclosed by the invention, have the characteristics of high accuracy, strong specificity ad high sensitivity, and KPN (Klebsiella Pneumoniae) and KPC in sputum and a throat swab sample can be rapidly and accurately detected.

The invention discloses a DNA chip and constructing method to detect the expression level of APEC and UPEC virulent gene in the sick human-animal technical domain, which comprises the following steps: augumenting the 97 gene segments of the known virulent gene and partial drug tolerant gene of escherichia coli; using SSH method to acquire 44 gene segments of APEC specific gene; adopting SCOTS method to obtain 13 gene segments of APEC specific gene; making the recombinant plasmid as mould; recycling and purifying each gene segment; using SmartArrayTM microarrayer to spot on chemically decorative the slide; setting positive and negative contrast; constructing the DNA chip with 154 target gene probes; fitting for detecting the expression level of APEC and UPEC virulent gene.

The invention discloses a gene detection probe and a gene detection method. The gene detection probe comprises inactivated nuclease protein, a marking molecule and guide RNA, and the marking moleculeand the guide RNA are connected to the inactivated nuclease protein which is inactivated Cas9 protein or inactivated Cpf1 protein. The gene detection method includes: subjecting a to-be-detected geneand the gene detection probe to incubation at the room temperature to obtain a gene-probe complex, and performing signal detection on the obtained gene-probe complex to realize gene detection. The gene detection probe can be hybridized with the to-be-detected gene at the normal temperature and is high in to-be-detected gene affinity and recognition specificity, high in hybridization speed and highin sensitivity, and quickness and accuracy in gene detection are realized.

The invention relates to a gene detection kit for prognosing gastric cancer metastasis. The kit comprises a DNA library building kit, wherein the DNA library building kit comprises a high-risk gene probe and a low-risk gene probe; the high-risk gene probe comprises CDH1, CDH2, SNAIL, SLUG, MUC4, MUC6, PRSS3, USP6, MLH1, MSH2, MSH6, PMS2, TGFBR2, MMP2, MMP9, BRCA1, BRCA2, PALB2, ATM, ATR, MUTYH, EMSY, ERCC4, RAD51, PARP1 and XRCC1; the low-risk gene probe comprises ATRX, BRIP1, FANCA, FANCB, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL, FANCM, FANCO, FANCP, MDM2, MDM4, MLH1, NPM1, PP2R1A, PRKDC, RAD50, STAG2, XRCC5 and CRCC6. The invention further discloses a use method of the kit. The use method comprises the following steps: extracting cfDNA in a blood sample; building a library for the cfDNA through the DNA library building kit, and then sequencing the DNA to obtain a gene overall length sequence; carrying out gene mutation analysis on the gene overall length sequence.

The present invention relates to an assay for identification of PCB dechlorinating organisms that are capable of biologically removing PCBs from contaminated materials. Specifically, the invention provides a set of primers for detecting PCB dechlorinating organisms in a sample. These individual primers of the primer set have a sequence of at least 12 nucleotides that is unique to 16S rDNA of PCB dechlorinating organisms.

The invention belongs to the technical field of virus detection kit, and provides a human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit, and a detection method thereof. The human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit comprises human parainfluenza virus specific primers and gene probes; and in the detection method, the human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit is used for detection. Compared with the prior art, the human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit and the detection method possess following advantages: detection of human parainfluenza virus I, II, III, and IV types can be realized at the same time; sensitivity, specificity, and accuracy are high; and detection period is short.

Disclosed are nucleic acid and amino acid sequences encoded by a novel Neuroendocrine-like marker (NELM) and diagnostic techniques for the detection of human prostate cancer utilizing such nucleic acid and amino acid sequences. Genetic probes and methods useful in monitoring the progression, diagnosis, and response to therapy of prostate cancer, as well as identifying compounds that promote prostate cancer are described.

The invention relates to the technical field of security detection of agricultural and sideline products, in particular to a primer for simultaneously detecting five food-borne pathogenic bacteria through a liquid chip and a detection method. The detection method comprises steps as follows: a specific primer and a gene probe for five foodborne pathogenic bacteria are designed; DNA templates of the detected five foodborne pathogenic bacteria are prepared; PCR (polymerase chain reaction) amplification is performed on the five foodborne pathogenic bacteria; coupling is performed on the gene probe for the five foodborne pathogenic bacteria and microspheres, and a microsphere probe is obtained and verified; an obtained PCR amplification product and the microsphere probe have a hybridization reaction; a fluorescence signal of a hybridized product is detected, and quantitative analysis is performed on the five foodborne pathogenic bacteria. A method capable of simultaneously detecting five common foodborne pathogenic bacteria is successfully established, the variable coefficient is within 2%, the specificity is 100%, the sensitivity can reach 100 CFU / mL, and the method is equivalent to a fluorescent PCR technology and can be applied to high-throughput rapid detection of clinic and environment specimens of foodborne diseases.