Marking probe of nano microparticle and affinity element and its preparation method as well as application

Abstract

A nanopartide and its avidin probe are disclosed. Said gene probe for detecting the mutation or non-mutation of DNA is prepared from nanoparticles or colloidal particles and avidin, streptavidin, or antibiotin through preparing colloidal gold and preparing the compound. Its advantges are high sensitivity, specificity and speed.

Description

technical field [0001] The invention relates to a gene detection technology, more specifically, to a nanometer or colloid particle and avidin complex labeling probe and a preparation method thereof, and a method for detecting DNA by combining the probe with silver enhancement / gold enhancement. Background technique [0002] Gene research has driven the rapid development of the entire life science in the 20th century; in the 21st century, gene research will still promote the in-depth development of life science research. Gene detection is not only crucial to biological research, but also to clinical medicine, pharmacy, agricultural technology, Environmental monitoring, legal identification and other fields are also of great significance. [0003] Genetic testing generally uses direct sequencing or hybridization testing. Hybridization detection includes radiation method, enzyme labeling method, chemiluminescence labeling method and so on. Radiation method has high sensitivity...

AI Technical Summary

Problems solved by technology

Due to the complex reaction process of the enzyme-labeled method, the enzyme-catalyzed substrate reacts too quickly, and the color area is not concentrated, which limits its use, especially not suitable for applications in the field of biochips

Fluorescence detection has the disadvantages of complex process, expensive detection equipment, operators need to be trained, which makes it difficult to popularize and apply.

[0004] Chemiluminescent labeling, common silver staining and some other labeling methods have limited applications due to their low sensitivity

So far, the relatively mature gene chip detection method is laser scanning confocal microscopy, but its procedure is complicated, the detection equipment is expensive, and it is difficult to promote and apply

[0005] Chinese patent CN1339609A discloses a nanoparticle-labeled gene probe and its application in gene detection. Due to the instability and easy aggregation of the prepared nanoparticle, this technology has the disadvantages of high production cost, poor stability and the use of inconvenient defect

However, since the problem of connection between nanoparticles and DNA and its preservation have not been fundamentally resolved, there are still practical difficulties in its promotion and use.

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