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SNP molecular marker for CFL1 gene of Qinchuan cattle and detection method of SNP molecular marker

A technology of molecular markers and detection methods, applied in the field of molecular genetics, can solve problems such as lack of research

Active Publication Date: 2019-12-13
YANGZHOU UNIV
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Problems solved by technology

Due to the lack of research on the genetic variation of the CFL1 gene in Qinchuan cattle in China, the research on the function of this gene locus and the relationship between the genetic variation of this gene and economic traits (such as: meat production, growth and development, etc.) have become blank

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  • SNP molecular marker for CFL1 gene of Qinchuan cattle and detection method of SNP molecular marker
  • SNP molecular marker for CFL1 gene of Qinchuan cattle and detection method of SNP molecular marker
  • SNP molecular marker for CFL1 gene of Qinchuan cattle and detection method of SNP molecular marker

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Embodiment Construction

[0039] The invention uses the conserved sequence of the CFL1 gene to design a primer pair P to amplify the 395 bp fragment of the second exon of the CFL1 gene, and uses Qinchuan cattle genome DNA as a template to carry out PCR amplification, and the amplified product is sequenced to find the identity of the amplified fragment Single nucleotide polymorphism sites; for the found single nucleotide polymorphism sites, the association analysis of growth traits is carried out, and its detection methods are provided, making the nucleotide polymorphism of the CFL1 gene a fast and convenient method. The detected molecular genetic markers provide a basis for accelerating the establishment of Qinchuan cattle populations with high-quality economic traits.

[0040] a. Detection of CFL1 gene polymorphism in Qinchuan cattle

[0041] 1. Collection and processing of Qinchuan cattle blood samples

[0042] Take Qinchuan cattle blood sample 10mL, add 0.5mol / L EDTA 500μL anticoagulant, slowly inv...

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Abstract

The invention belongs to the field of molecular genetics, and provides an SNP molecular marker for CF1 gene of Qinchuan cattle and a detection method of the SNP molecular marker. The whole genome DNAof Qinchuan cattle to be tested containing the CFL1 gene is used as a template, a primer pair P is used as primers, and PCR is carried out to amplify the CFL1 gene; agarose gel electrophoresis is performed on the amplified fragments after enzyme digestion, after the PCR amplified product is digested with a restriction enzyme HinfI; and the base polymorphism at position 2052 of the CFL1 gene of Qinchuan cattle is identified according to the electrophoresis results. Because the mutation site is closely related to the beef traits (body length, chest width, chest depth and weight) of Qinchuan cattle, and the method is a method for screening and detecting the molecular genetic markers closely related to the growth traits of Qinchuan cattle at DNA level, the SNP molecular marker and the method can be used in auxiliary selection and molecular breeding of Qinchuan cattle, so as to accelerate the breeding speed of Qinchuan cattle.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to a single nucleotide polymorphism (SNP) of a functional gene of Qinchuan cattle as a molecular genetic marker, in particular to a SNP molecular marker of the CFL1 gene of Qinchuan cattle and a detection method thereof. Background technique [0002] In beef cattle breeding, people expect to achieve the purpose of early selection and improve the accuracy of breeding values ​​through the selection of DNA markers that are closely related to growth traits and closely linked to quantitative traits, so as to obtain greater heredity in livestock and poultry breeding. progress. [0003] Molecular breeding, that is, Molecular Mark-Assist Selection (MAS), is a technology that uses DNA molecular markers to select genetic resources or breeding materials, and to improve the comprehensive traits of livestock and poultry. It uses modern molecular The method of combining biology and traditional geneti...

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 孙雨佳徐崇杨章平
Owner YANGZHOU UNIV
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