Racomitrium canescens protein phosphatase 2C gene RcPP2C as well as encoded protein and application thereof

A protein phosphatase, C gene technology, applied in the fields of genes and their encoded proteins and applications, can solve the problem of few bryophytes research

Active Publication Date: 2018-03-02
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] With the rapid development of molecular biology, signal transduction-related genes have been studied in depth in plants such as rice, corn, and tobacco, but there are few related studies in bryophytes

Method used

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  • Racomitrium canescens protein phosphatase 2C gene RcPP2C as well as encoded protein and application thereof
  • Racomitrium canescens protein phosphatase 2C gene RcPP2C as well as encoded protein and application thereof
  • Racomitrium canescens protein phosphatase 2C gene RcPP2C as well as encoded protein and application thereof

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specific Embodiment approach 1

[0025] Embodiment 1: The nucleotide sequence of the moss protein phosphatase 2C gene RcPP2C in this embodiment is shown in Seq ID No: 1 in the sequence table.

[0026] The moss protein phosphatase 2C gene RcPP2C provided in this embodiment is from moss moss. Using sand moss as the experimental material, the aquaporin gene related to drought resistance was cloned and transgenic, and drought-resistant transgenic plants have been obtained, which can provide excellent genetic resources for plant genetic engineering breeding and provide a material basis for improving plant stress resistance .

specific Embodiment approach 2

[0027] Specific embodiment 2: The amino acid sequence of this embodiment encoding the moss protein phosphatase 2C gene RcPP2C protein is shown in the sequence table Seq ID No: 2.

specific Embodiment approach 3

[0028] Specific Embodiment Three: The application of the moss protein phosphatase 2C gene RcPP2C gene in this embodiment refers to the application in improving the drought resistance of plants.

[0029] Verify effect of the present invention by following experiment:

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Abstract

The invention relates to a racomitrium canescens protein phosphatase 2C gene RcPP2C as well as encoded protein and an application thereof. The invention aims at providing the racomitrium canescens protein phosphatase 2C gene RcPP2C as well as the encoded protein and the application thereof. The sequence of the racomitrium canescens protein phosphatase 2C gene RcPP2C is shown as SEQ ID No: 1 in a sequence list, and an amino acid sequence is shown as SEQ ID No.2 in the sequence list; and the gene is applied to the improvement of plant drought resistance. The RcPP2C gene is related to drought resistance in a cell signal transduction pathway; the gene, when transformed into a plant, can improve the drought resistance of the plant and the gene can lay a foundation for breeding a novel drought-resistant plant variety and enhancing the drought resistance of the plant; therefore, the gene has important significance. The gene provided by the invention is applied to the field of molecular breeding.

Description

technical field [0001] The invention relates to a gene, its encoded protein and its application, in particular to the moss protein phosphatase 2C gene RcPP2C, its encoded protein and its application. Background technique [0002] Water stress such as drought, high salinity and low temperature is the most important environmental stress factor affecting plant growth and development. Water scarcity has become a major handicap to agricultural development worldwide. In order to solve this problem, people are trying to find drought-tolerant genes from resource-rich wild plant gene pools, and use advanced biotechnology to breed new plant varieties that are resistant to salt, drought, and low temperature, and enhance the stress resistance of plants, thereby Increase economic value. Therefore, how to improve the stress resistance of plants has become a research hotspot for scientists from all over the world. [0003] Signaling pathway means that when a cell needs to respond in a c...

Claims

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Application Information

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IPC IPC(8): C12N15/55C12N9/16C12N15/82A01H5/00A01H6/82
CPCC12N9/16C12N15/8273C12Y301/03
Inventor 沙伟张梅娟张春蕾
Owner QIQIHAR UNIVERSITY
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