Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Screening method of goat COX II gene segment capable of serving as molecular marker and application thereof

A technology of gene fragment and molecular marker, applied in the field of livestock molecular marker preparation

Inactive Publication Date: 2012-07-04
ANHUI AGRICULTURAL UNIVERSITY +1
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on COX II gene mainly focuses on the regulation of liver, heart and other tissue cells, and the research on the effect of COX II gene mutation on goat sperm motility has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Screening method of goat COX II gene segment capable of serving as molecular marker and application thereof
  • Screening method of goat COX II gene segment capable of serving as molecular marker and application thereof
  • Screening method of goat COX II gene segment capable of serving as molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] figure 1 The operation process of this embodiment is shown, and the present invention will be further described below in conjunction with the embodiment.

[0024] 1. Amplification of partial DNA fragments of COX II gene related to goat sperm motility traits

[0025] 1.1 Sample collection and genomic DNA extraction

[0026] Sperm collection is performed by the pseudo-vaginal method. The rams collected for semen were strong and good in reproductive performance Anhui white goats (40) and Boer goats (40). The samples were provided by the breeding farm of Hefei Boda Animal Husbandry Technology Company. Store frozen in a -70°C freezer. Genomic DNA was extracted according to the following methods:

[0027] 1) Take 1ml of goat semen and put it into a 2ml high-pressure sterilized centrifuge tube, add 1ml of PBS to rinse, discard the supernatant, then add 1ml of PBS to rinse once, and discard the supernatant.

[0028] 2) Add 700 μl of TE extract to the centrifuge tube, then ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Amplified lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a screening method of a goat COX II gene segment capable of serving as a molecular marker and application thereof. The screening method comprises the following steps: by using constructed goat sperm pool DNA (deoxyribonucleic acid) as a template, using a designed specific primer P1 set a corresponding program in the presence of Tag DNA polymerase, buffer, Mg<2+> and dNTPs (deoxyribonucleotide triphosphates), and carrying out PCR (polymerase chain reaction) amplification on the COX II gene; determining the size of the target segment according to an agarose gel electrophoretogram; sequencing to obtain sequence and mutant site information, wherein the result indicates that the amplification product has one base mutant; after digesting HindI II the P1 amplification product with restriction endonuclease, detecting the digested amplification segment by agarose gel electrophoresis; and analyzing the genotype and gene frequency of the P1 amplification product as well as the correlation of the sperm activities between the Anhwei white goat and Bohr goat, wherein the result indicates that the SNP (single nucleotide polymorphism) site of the COX II gene detected by the primer P1 can serve as a molecular marker for selecting goat sperm activity property, thereby providing a molecular marker for auxiliary selection of a goat sperm quality property marker.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular marker preparation, in particular to a screening method and application of a goat COX II gene fragment which can be used as a molecular marker. Background technique [0002] Sheep semen freezing and artificial insemination is a mature biotechnology that has been put into production. It has become an indispensable technology for the breeding of new varieties and the promotion of improved varieties, and plays a huge role in the improvement and production of goat varieties. Artificial insemination of sheep is a method in which ram semen is collected mechanically, and after quality inspection and dilution treatment, the semen is injected into the reproductive tract of estrous ewes to replace the natural mating of rams and ewes. The use of artificial insemination has the following advantages: (1) Improve the breeding efficiency of rams, especially in the case of frozen semen, one ram can ma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 凌英会丁建平章孝荣黄桠峰张晓东张子军张运海孙志辉章孝斌
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com