Single nucleotide polymorphism of SCD genes in dairy goat and detection method thereof
A kind of technology of single nucleotide polymorphism, detection method
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[0034]The invention uses the conservative sequence of the SCD gene to design primers to amplify the 5th exon and the 362bp fragment of the flanking region of the SCD gene, respectively use the genomes of two dairy goat varieties as templates to perform PCR amplification, and purify the PCR products. After sequencing Find the single nucleotide polymorphism of the amplified fragment; perform a trait correlation analysis on the found single nucleotide polymorphism, and provide its detection method, making the nucleotide polymorphism of the SCD gene a fast, Molecular genetic markers for convenient detection provide a basis for accelerating the establishment of dairy goat populations with high-quality economic traits.
[0035] a. Cloning of partial DNA sequence of dairy goat SCD gene and detection of its polymorphism
[0036] 1. Collection and processing of dairy goat blood samples
[0037] Take 10 mL of milk goat blood sample, add 500 μL of 0.5 mol / L EDTA for anticoagulation, slo...
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