Qinchuan cattle FoxO1 gene mononucleotide polymorphism molecular marker detection method and application

Abstract

The invention discloses cattle FoxO1 gene mononucleotide polymorphism and a detection method thereof. The gene mononucleotide polymorphism comprises that: with a Qinchuan cattle whole genome DNA comprising the FoxO1 gene as a template, and with a primer pair P as primers, the Qinchuan cattle FoxO1 gene is subjected to PCR amplification; a restriction endonuclease HhaI is used for digesting the PCR amplification product, and the digested segment is subjected to agarose gel electrophoresis; and according to the electrophoresis result, base polymorphism on the 178132 site of the Qinchuan cattle FoxO1 gene is identified. The FoxO1 gene is important for animal muscle fat traits and growth traits. With the method, the molecular genetic marker closely related to Qinchuan cattle growth traits is screened and detected on a DNA level. The method is used in Qinchuan cattle assisted selection and molecular breeding, and assists in accelerating Qinchuan cattle breeding speed.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a single nucleotide polymorphism (SNP) of a functional gene of Qinchuan cattle as a molecular genetic marker, in particular to a single nucleotide polymorphism of the FoxO1 gene of Qinchuan cattle and its detection method. Background technique [0002] In beef cattle breeding, people expect to achieve the purpose of early selection and improve the accuracy of breeding values ​​through the selection of DNA markers that are closely related to growth traits and closely linked to quantitative traits, so as to obtain greater heredity in livestock and poultry breeding. progress. [0003] Molecular breeding, that is, Molecular Mark-Assist Selection (MAS), is a technology that uses DNA molecular markers to select genetic resources or breeding materials, and to improve the comprehensive traits of livestock and poultry. It uses modern molecular The method of combining biology and traditio...

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Problems solved by technology

Due to the lack of research on the genetic variation of the FoxO1 gene in Qinchuan cattle in China, the research on the function of this gene locus and the relationship between the genetic variation of this gene and economic traits (such as: meat production, growth and development, etc.) have become blank

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