Method for detecting single nucleotide polymorphism of sheep PCNP (PEST-Containing Nuclear Protein) gene by using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) and application of method
A single nucleotide polymorphism, sheep technology, applied in the field of molecular genetics, can solve problems such as lack of research, achieve the effect of improving selection efficiency, simple method, and speeding up breeding progress
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[0033] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, and the described embodiments are explanations of the present invention, rather than limitations.
[0034] The invention uses the conserved sequence of the PCNP gene to design primers to amplify the 686bp fragment of the second exon of the PCNP gene, and uses the genomes of Hu sheep and Small-tailed Han sheep as templates to perform PCR amplification. Nucleotide polymorphism: To conduct trait correlation analysis for the discovered single nucleotide polymorphism, and provide its detection method, making the nucleotide polymorphism of the PCNP gene a molecular genetic marker that can be quickly and conveniently detected, It provides a basis for accelerating the establishment of sheep populations such as Hu sheep and small-tailed Han sheep with high-quality economic traits.
[0035] a. Detection of PCNP gene polymorphism in sheep
[0036] 1. Coll...
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