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Single nucleotide polymorphic locus of cattle I-mfa gene and detection method thereof

A single nucleotide polymorphism and polymorphic site technology, applied in the field of molecular genetics, can solve problems such as lack of research, and achieve the effect of improving selection efficiency, high accuracy, and low cost

Inactive Publication Date: 2012-09-12
XUZHOU NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Due to the lack of research in the field of genetic variation of the cattle I-mfa gene, the research on the function of the gene locus and the relationship between the genetic variation of the gene and economic traits (such as: growth and development traits) have become blank

Method used

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  • Single nucleotide polymorphic locus of cattle I-mfa gene and detection method thereof
  • Single nucleotide polymorphic locus of cattle I-mfa gene and detection method thereof
  • Single nucleotide polymorphic locus of cattle I-mfa gene and detection method thereof

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Embodiment Construction

[0036] In order to facilitate the understanding of the technical solution of the present invention, the present invention will be further described below in conjunction with specific examples.

[0037] In the present invention, primers are designed based on the conservative sequence of I-mfa gene, and the genomic DNAs of three cattle breeds are respectively used as templates to carry out PCR amplification, and the PCR products are mixed, purified and sequenced. Then, carry out sequencing map analysis and sequence comparison to screen out SNP sites; secondly, carry out PCR-SSCP detection of polymorphic sites in the tested population; finally, carry out statistical analysis of population genetics according to the genotypes detected in the population The association analysis with growth traits screened out the molecular markers closely related to the growth traits of cattle. The present invention will be described in detail below, which is an explanation of the present invention ...

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Abstract

The invention discloses a single nucleotide polymorphic locus of a cattle I-mfa gene and a detection method thereof. The detection method of the single nucleotide polymorphisms locus of cattle I-mfa gene comprises utilizing cattle whole-genome deoxyribonucleic acid (DNA) to be detected containing the I-mfa gene as a template, utilizing a primer pair P as primers, performing polymerase chain reaction (PCR) amplification on the cattle I-mfa gene and performing polyacrylamide gel electrophoresis on amplified framorphism; according to electrophoresis results, identifying the 12284 locus of the cattle I-mfa gene to be nucleotide polymplate of A or G; and identifying the 12331 locus of the cattle I-mfa gene to be nucleotide polymorphism of T or C. The two locus is completely in linkage. The detection method of the single nucleotide polymorphic locus of the cattle I-mfa gene is a method which screens and detects molecular genetic markers closely related to growth traits of cattle from the DNA level, and provides the molecular basis for cattle crossbreeding utilizing the crossbreed advantages.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to a single nucleotide polymorphism (SNP) of a functional gene of cattle as a molecular genetic marker, in particular to a single nucleotide polymorphism site of the I-mfa gene of cattle and its detection method. Background technique [0002] Cattle are one of the main livestock in my country, but now they are facing the pressure of insufficient provenance of excellent cattle breeds and poor genetic quality of the population. Traditional breeding methods apply the phenotypes of test animals and the genetic information of their parents, grandparents and other relatives in small animal models, assuming that traits are affected by genetic differences in many genes, each of which makes a relatively small contribution to the trait, Therefore, some biases are inevitable in the estimation of traits. Molecular genetic markers are one of the fastest-growing fields in modern genetics in rece...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 陈宏李景景张春雷房兴堂
Owner XUZHOU NORMAL UNIVERSITY
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