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Detection method for single nucleotide polymorphism of STMN1 gene of Beijing duck and molecular markers thereof

A technology of single nucleotide polymorphism and detection method, which is applied in the field of single nucleotide polymorphism of Beijing duck STMN1 gene and its detection, can solve problems such as lack of research, and achieve the effect of improving selection efficiency and accelerating breeding progress.

Inactive Publication Date: 2012-12-12
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

Due to the lack of research on the genetic variation of the STMN1 gene in Peking ducks, the functional research of this gene locus and the research on the relationship between the genetic variation of this gene and economic traits are blank.

Method used

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  • Detection method for single nucleotide polymorphism of STMN1 gene of Beijing duck and molecular markers thereof
  • Detection method for single nucleotide polymorphism of STMN1 gene of Beijing duck and molecular markers thereof
  • Detection method for single nucleotide polymorphism of STMN1 gene of Beijing duck and molecular markers thereof

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Embodiment Construction

[0026] The invention uses the conserved sequence of the STMN1 gene to design primers to amplify the 747bp fragment of exon 4 of the STMN1 gene, and uses the genome of the Peking duck variety as a template to perform PCR amplification, purify the PCR product, and search for the amplified fragment after sequencing single nucleotide polymorphisms; for the found single nucleotide polymorphisms, the trait association analysis is carried out, and its detection method is provided, so that the nucleotide polymorphisms of the STMN1 gene become a molecular genetic method that can be quickly and conveniently detected To provide a basis for accelerating the establishment of Peking duck populations with high-quality economic traits.

[0027] a. Cloning of partial DNA sequence of Peking duck STMN1 gene and detection of its polymorphism

[0028] 1. Collection and processing of Peking duck blood samples

[0029] Take 6 mL of Peking duck blood sample, add 0.5 mol / L anticoagulant ACD 1 mL to a...

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Abstract

The invention discloses single nucleotide polymorphism of the STMN1 gene of a Beijing duck and a detection method thereof. The detection method for the single nucleotide polymorphism of the gene comprises the following steps: with whole genome DNA of a to-be-detected Beijing duck containing the STMN1 gene as a template and the primer pair P as primers, carrying out PCR amplification on the STMN1 gene of the Beijing duck; digesting a PCR amplification product with restriction endonuclease and then carrying out agarose gel electrophoresis on amplified segment after digestion; and identifying polymorphism of the 48th nucleotide base of exon 4 of the STMN1 gene of the Beijing duck according to results of electrophoresis. According to the invention, molecular genetic markers closely related to economic characters of the Beijing duck are screened and detected at the level of DNA, which provides molecular bases for rapid selective breeding of the Beijing duck.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to using the single nucleotide polymorphism (SNP) of the functional gene of Peking duck as a molecular genetic marker, in particular to the single nucleotide polymorphism of the Peking duck STMN1 gene and a detection method thereof. Background technique [0002] Single nucleotide polymorphism (SNP) refers to the polymorphism caused by the substitution of a single nucleotide (A / T / C / G) in the genomic DNA sequence. Therefore, commonly referred to as SNPs include base substitutions, insertions, deletions, and changes in the copy number of repeated sequences. A SNP indicates that there is a nucleotide change at a certain position in the genome, which is mainly caused by the conversion or transversion of a single base; SNPs with conversion mutations account for about 2 / 3, and other SNPs are similar level. The cytosine of the CpG dinucleotide is the most mutated site in the genome, most o...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68A01K67/027
Inventor 陈宏张海燕张春雷房兴堂
Owner XUZHOU NORMAL UNIVERSITY
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