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Meat sheep reproductive performance SNP locus combination and application thereof

A technology for reproductive performance and sheep, applied in the direction of recombinant DNA technology, DNA/RNA fragments, microbial measurement/inspection, etc., can solve the problems of insufficient uniformity of sites, insufficient expression of functional sites and regions, etc., and shorten generations Spacing, saving breeding cost, good versatility

Active Publication Date: 2021-04-23
中科云谱(内蒙古)基因生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing sheep SNP chip is mainly based on the data of western sheep breeds, lacks the SNP data of the combination of Chinese sheep breeds and foreign sheep breeds, and has insufficient locus uniformity, insufficient reflection of functional loci and regions, and more than 10%. There are many problems such as extremely low-frequency loci in Chinese sheep populations. It is of great significance to design a SNP chip that is suitable for Chinese sheep populations and can quickly and effectively detect reproductive function.

Method used

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  • Meat sheep reproductive performance SNP locus combination and application thereof
  • Meat sheep reproductive performance SNP locus combination and application thereof
  • Meat sheep reproductive performance SNP locus combination and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Combination of SNP loci for reproductive performance of meat sheep

[0046] 1. Acquisition of the total SNP set

[0047] A total of 248 meat sheep individuals of 16 Asian mouflons, 172 local breeds and 60 cultivated breeds covering Asia, Europe, Africa and the Middle East were sequenced at a high depth, with an average sequencing depth of ~25.7×. The sequence analysis process is compared with the sheep 4.0 reference genome (obtained from NCBI) released in 2015 through SAMtools and GATK. The common results obtained by the two methods form a SNP set, which contains a total of 28.36 million SNP bits point. Specifically, the high-depth sequencing of multiple meat sheep individuals is completed by Bio-Sequencing Company, and the sequencing results completed by Bio-Sequencing Company can all achieve the technical purpose of the present invention, which is not limited by the present invention. This application compares the Fastq file returned by the sequencing comp...

Embodiment 2

[0054] Example 2 Preparation of SNP Gene Chip for Meat Sheep Reproductive Performance

[0055] Based on the combination of SNP sites obtained in Example 1, those skilled in the art can use any method to prepare SNP gene chips for the reproductive performance of meat sheep, and can also entrust biological companies to prepare them. The present invention is not limited, such as Illumina, Affymetrix Company or Agilent Company, it should be noted that the gene chips prepared based on the SNP sites provided in Example 1 of the present invention all belong to the protection scope of the present invention.

[0056] In one embodiment of the present invention, the method of Illumina Company can be used to prepare the SNP chip of the breeding performance of meat sheep of the present application, the chip includes a glass substrate and 2917 magnetic beads located in the small holes of the substrate, said Each magnetic bead is coupled with probes for respectively detecting the sites shown...

Embodiment 3

[0057] Example 3 SNP detection kit

[0058] Based on the combination of SNP sites obtained in Example 1, those skilled in the art can use any method to prepare the SNP detection kit for the reproductive performance of meat sheep, and can also entrust a biological company to prepare it. The present invention is not limited, such as Illumina , Affymetrix or Agilent, it should be noted that the detection kits prepared based on the SNP sites provided in Example 1 of the present invention all belong to the protection scope of the present invention.

[0059] In one embodiment of the present invention, in order to use the gene chip provided in Example 2 to detect the target sample, the kit also includes four kinds of labeled dideoxynucleotides, polymerase and biotin-labeled antibody Streptavidin antibody, and DNP-labeled antibody against the FC end of the heterologous antibody.

[0060] Of course, different gene chips should be adapted to corresponding reagents, which is not limited...

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Abstract

The invention discloses a meat sheep reproductive performance SNP locus combination and an application thereof, and relates to the technical field of gene breeding. The locus combination provided by the invention comprises 2917 SNP loci, the physical position information of the SNP loci is determined based on sheep v4.0 genome sequence comparison, the SNP loci are SNP0001-SNP2917, and genetic evaluation is performed on sheep individuals based on the 2917 SNP locus combination. Individual selection can be performed on reproductive traits difficult to measure in the early stage, the generation interval is shortened, the breeding process is accelerated, and therefore, a large amount of breeding cost is saved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a combination of SNP loci for reproductive performance of meat sheep and its application. Background technique [0002] Sheep is the earliest domesticated animal and is also the most common farmed animal in my country. With human activities and selection, sheep breeds with different high-quality resources have gradually formed. However, in actual production, there are many types of sheep at home and abroad. The difference is large, and it has become one of the major problems in sheep farming to select breeds with specific target traits for breeding and production, thereby obtaining higher economic value or market benefits. [0003] In the history of selective breeding of sheep, it has experienced from empirical breeding to the exploration of breeding theory and methods, including selection theory, pure line theory, backcross breeding, recurrent breeding, mutation breeding, single grai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6837C12N15/11
CPCC12Q1/6837C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李孟华李心罗凌云杨继吕锋骅
Owner 中科云谱(内蒙古)基因生物科技有限公司
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