37results about How to "Achieve early selection" patented technology

The invention relates to the technical field of animal molecular markers, in particular to a molecular marker related with a diameter character of sheep wool fibers as well as a specific primer and application thereof in selection of diameter character of Chinese Merino sheep wool fibers. The molecular marker related with the diameter character of the sheep wool fibers is located at the No.30 exonof the No.21 chromosome of FAT3 gene. The molecular marker has the advantages that the function of using rs425144268 SNP site as the molecular marker of the diameter of the sheep wool fibers is disclosed for the first time, and the function of applying the rs425144268 SNP site to the selection of the diameter character of the Chinese Merino sheep wool fibers is disclosed for the first time; whenthe excellent diameter character of the wool fibers is identified by the molecular marker related with the diameter character of the sheep wool fibers, the operation is simple, and the sheep with smaller fiber diameter can be screened in an assisting way; because the detection level of the molecular marker is started, the accuracy of variety selection is improved, and the detection efficiency is improved.

The invention relates to the technical field of animal molecular markers, in particular to a molecular marker capable of predicting and identifying a sheep wool length and application of a specific primer pair in predicting and identifying wool length character of Chinese Merino lambs. The molecular marker capable of predicting and identifying the sheep wool length is located at a first exon of aNo. 1 chromosome of IFNAR2 genes. The invention firstly discloses an rs407032027 SNP locus serving as a molecular maker capable of predicting and identifying the sheep wool length, and firstly discloses the application of the rs407032027 SNP locus in the selection of the wool length character of the Chinese Merino lamb; and when the molecular marker capable of predicting and identifying the sheepwool length is adopted to select a sheep variety with excellent wool length character, the molecular marker has the advantages of simple operation, low expense, high accuracy and capability of automatic detection.

The invention discloses a molecular marker and specific primers for assisting in test of wilt disease resistance in brassica oleracea and use thereof. The invention provides a reagent for assisting in the test of wilt disease resistance in brassica oleracea and/or assisting in screening brassica oleracea with wilt disease resistance, which is a specific primer pair formed by nucleotides represented by a sequence 2 and a sequence 3 in a sequence table. The invention also provides a specific gene fragment which is at a genetic distance about 2.87cM to the wilt disease resistance gene in brassica oleracea and is formed by nucleotides represented by a sequence 1 in a sequence table. The result of the identification of wilt disease resistance in brassica oleracea and/or screening of the brassica oleracea with wilt disease resistance with assistance from the reagent (primer pair) or sequence characterized amplified region (SCAR) marker, which are provided by the invention, is 97 percent consistent with that of field identification. When used in breeding, the reagent, molecular marker or method has the advantages of accuracy, quickness, capability of realizing early breeding and the like and has a bright application prospect.

The invention relates to the field of molecular genetics, in particular to the application of a molecular marker method in pig breeding for disease resistance, wherein according to the molecular marker method, the molecule at a mutation site in a CYP3A88 gene 5' regulatory region of a pig is marked. The inventor of the method discovers that the CYP3A885' regulatory region of a large Chinese streaky-head pig and the CYP3A885' regulatory region of a Duroc long hybrid pig have a plurality of differences, wherein an A-to-T mutation exists at the -78 site, through a luciferase reporter gene system, the fact that promoter activity of the -78 site is lowered remarkably after an A at the -78 site is mutated into a T in a site-directed mutagenesis mode is found, and the promoter activity of the -78 site is the same as the low level of messenger ribonucleic acid (mRNA) expression of a CYP3A88 gene of the porcine reproductive and respiratory syndrome (PRRS)-resistant large Chinese streaky-head pig. Therefore, through genotype detection of the -78 site of the CYP3A88 regulatory region in a pig genome, the genotype of the -78 site of the CYP3A88 regulatory region can be used as a modular marker associated with traits of the PPRS, the molecular marker method not only is simple, convenient and rapid, but also cannot be affected by the environment, and early selection for breeding can be realized.

The invention relates to a molecular marking method, in particular to a molecular marking method for predicting and identifying the length of sheep wool. The method comprises the steps as follows: 1), extracting sheep genome DNA, designing a primer, performing PCR (polymerase chain reaction) amplification, performing enzyme digestion, and obtaining an enzyme digestion product; 2), subjecting the enzyme digestion product to electrophoretic separation, and judging genotypes according to an electrophoretic separation result; 3), performing association analysis and estimating the least square mean value of characters, and obtaining a result that the length of wool of a GG genotype individual is remarkably higher than that of wool of AA genotype and AG genotype individuals in the three genotypes; and 4), constructing wool length character breeding population taking the GG genotype individual as the primary so that the length of the sheep wool is predicated and identified. According to the method, the operation is simple, the cost is low, the accuracy is high, and automatic detection can be performed. Selective breeding of the sheep wool length is performed with the molecular marking method, so that a genetic breeding process of the length characters of the sheep wool can be accelerated, breeding sheep can be early selected, the sheep can be selected and left after birth, and a sheep breeding process is accelerated.

The invention relates to the field of molecular genetics, in particular to a molecular marker method for two mutation sites of a chicken MMP13 gene 5' control region and application of the molecular marker method in chicken breeding. It is found by the inventor that six mutation sites exist in the chicken MMP13 5' control region, namely, -1719 (T>C), -1661 (C>A), -1356 (G>A), -1128 (A>G), -1094 (C>A) and -1079 (T>C); linkage disequilibrium analysis is performed through the SHEsis online software, and it is found through the result that the sites -1719 and -1661, the sites -1356 and -1128 and the sites -1094 and -1079 are respectively in a completely-linked state in White Recessive Rock. The method is easy, convenient and fast to implement, beneficial for breeding chicken breeds laying eggs early at a high yield and capable of providing favorable help for the marker-assisted breeding work.

The invention relates to a molecular marking method for two mutation sites in a chicken PTHLH gene 5' regulatory region and application thereof in chicken breeding. Two mutation sites (namely the -1827 (A>G) site and the -165(C>T) site) exist in the chicken PTHLH gene 5' regulatory region, SHEsis online software is used for linkage disequilibrium analysis, and the result shows that the two sites in recessive white plymouth rock are in a complete linkage state. The two sites are related with the first-egg age and egg laying quantity at the 32th week, the first-egg age of AC/GT gene type is remarkably earlier than that of the GT/GT type and the AC/AC type, and meanwhile the egg laying quantity at the 32th week is remarkably larger than that of the other two types. The method is simple, fast and beneficial for breeding chicken variety with the early first-egg age and the large egg laying quantity and provides favorable help for marker-assisted breeding work.

The invention discloses a molecular marker relevant to the reproductive performance of chicken and application of the molecular marker in breeding, belonging to the field of molecular genetics. The nucleotide sequence of the molecular marker is shown in SEQ ID No.1; a C->T base substitution exists at the 315th bp (base pair) of the sequence, thereby leading polymorphism of SspI-RFLP. The genome DNA of chicken is taken as a template, and primers shown in SEQ IDNo.2-3 are used for amplifying to obtain a PCR amplification product of 556bp; the PCR product is detected by using 1.5 percent agarose gel electrophoresis after being digested by an SspI restriction enzyme to obtain three banding patterns of CC, CT and TT; in local varieties with small egg weights and poor egg laying performance, CC genetype individuals are for reserved. A method for detecting the molecular marker relevant to the reproductive performance of chicken is simple and rapid and is not influenced by the environment, and early strain selection can be realized.

The invention provides a molecular marker method capable of indicating and identifying curling degree of sheep wools and a primer pair for the molecular marker method. The primer pair comprises an upper primer SHCBP1-P1-F shown in the sequence chart Seq No.1 and a lower primer SHCBP1-P1-R shown in the sequence chart Seq No.2. The molecular marker method comprises the following steps: designing a primer pair, extracting a sheep genome DNA for amplification, and then performing digestion to obtain a digestion product; performing electrophoretic separation on the digestion product, and judging the genotypes according to the electrophoretic separation result; performing correlation analysis, estimating the least squares means of the character, wherein the result is that the wool fineness of a GG genotype group and a GT genotype group in three genotypes is obviously higher than that of a TT genotype group; dividing the test group into three types to complete the method. The molecular marker method can indicate and identify the curling degree of sheep wools, and can be effectively applied to the auxiliary molecular breeding field of high-curling-degree sheep.