CYP3A88-molecular-marker breeding method for sorting porcine reproductive and respiratory syndrome (PRRS)-resistant pigs and application thereof

Abstract

The invention relates to the field of molecular genetics, in particular to the application of a molecular marker method in pig breeding for disease resistance, wherein according to the molecular marker method, the molecule at a mutation site in a CYP3A88 gene 5' regulatory region of a pig is marked. The inventor of the method discovers that the CYP3A885' regulatory region of a large Chinese streaky-head pig and the CYP3A885' regulatory region of a Duroc long hybrid pig have a plurality of differences, wherein an A-to-T mutation exists at the -78 site, through a luciferase reporter gene system, the fact that promoter activity of the -78 site is lowered remarkably after an A at the -78 site is mutated into a T in a site-directed mutagenesis mode is found, and the promoter activity of the -78 site is the same as the low level of messenger ribonucleic acid (mRNA) expression of a CYP3A88 gene of the porcine reproductive and respiratory syndrome (PRRS)-resistant large Chinese streaky-head pig. Therefore, through genotype detection of the -78 site of the CYP3A88 regulatory region in a pig genome, the genotype of the -78 site of the CYP3A88 regulatory region can be used as a modular marker associated with traits of the PPRS, the molecular marker method not only is simple, convenient and rapid, but also cannot be affected by the environment, and early selection for breeding can be realized.

Description

technical field [0001] The present invention relates to the field of molecular genetics, in particular to a breeding method for screening pigs resistant to PRRS, by judging the polymorphism of the mutation site in the 5' regulatory region of the pig CYP3A88 gene to select the breed, and artificially applying the mutation The site realizes the breeding of pigs resistant to PRRS. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), also known as blue ear disease, is a porcine reproductive and respiratory disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) infection of porcine lung macrophages. The leading infectious disease affecting the swine industry worldwide (Neumann et al. 2005). The disease mainly causes reproductive disorders such as fever, premature birth, mummified fetuses and weak piglets in pregnant sows, as well as respiratory symptoms and high mortality in pigs of all ages (Rossow1998). The disease resista...

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Problems solved by technology

Petry et al. (2005) compared the differences between Nebraska index line and Hampshire-Duroc hybrid pigs after PRRSV infection, and detected the changes in body weight, rectal temperature and virus titer of these two pigs. The results showed that the Nebraska index line had strong resistance; Petry et al. (2007) also found that in these two pigs, there were differences in the expression levels of specific immune function genes and cytokines in lung and lymph node tissues; Bates et al. (2008) used gene chip technology to screen differentially expressed genes in the lungs and lymph nodes of these two pigs, and found that there were differences in the expression of a large number of genes

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