PCR primer set for identification or screening of cabbage hybrid lethal parent type 2 and its application

Abstract

The invention belongs to the technical field of molecular assisted breeding, in particular to a PCR primer set for identifying or screening cabbage hybrid lethal parent type 2 and its application. The PCR primer set includes primers KBoHL2-X, KBoHL2-Y, and KBoHL2-Common. The present invention detects whether the genotype at positions 46, 333, and 244 of the C04 chromosome of the cabbage to be tested is T / T or C / C, and determines whether the cabbage has the characteristics of hybrid lethal parent type 2 according to the genotype of the cabbage to be tested: T / T genotype of the cabbage to be tested It is the hybrid lethal parent type 2, and the tested cabbage of the C / C genotype is not the hybrid lethal parent type 2. Using the PCR primers, kit or method of the present invention for assisted breeding has the advantages of simple operation, strong specificity, good stability, and early breeding can be realized, and the coincidence rate of identification results and field phenotypes reaches 100%. It has great application prospect.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and plant genetics and breeding, and relates to a PCR primer set for identifying or screening dead parent type 2 of cabbage hybrids and an application thereof. Background technique [0002] Heading cabbage, referred to as cabbage, belongs to the Brassica cabbage species of the Cruciferae (Cruciferae). Cabbage has strong adaptability and stress resistance, and has high nutritional and health value, so it is widely cultivated in China and even all over the world. Hybrid lethality refers to the phenomenon that the parents grow normally, but the hybrids appear wilting, yellowing, dwarfed plants, or even die. Hybrid lethality is an important reproductive isolation method, which is very important for the formation of species and the maintenance of species purity. In recent years, there have been more and more studies on the lethality of plant hybrids, but in cruciferous crops, most of them a...

AI Technical Summary

Problems solved by technology

In recent years, there have been more and more studies on the lethality of plant hybrids, but in cruciferous crops, most of them are caused by intergeneric hybrids caused by distant hybridization, and the cases of intraspecific hybrid lethality are only in Arabidopsis thaliana. and Kale have been studied on

Cabbage hybrid lethality is an extreme abnormal phenomenon in the growth and development of cabbage, which will bring losses to the production of cabbage

Images