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Molecular marker related with diameter character of sheep wool fibers as well as specific primer and application thereof

A technology of wool fiber and molecular markers, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of inability to select early and rapid seed selection, and reduce the cost of breeding, The effect of simple operation and accelerated breeding process

Active Publication Date: 2018-01-19
新疆畜牧科学院畜牧研究所 +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a molecular marker related to the trait of sheep wool fiber diameter and its specific primer pair, and the specific primer pair is used as a preparation or kit for preparing in vitro detection of molecular markers related to the trait of sheep wool fiber diameter. Application, the application of the molecular markers in the selection of Chinese Merino sheep wool fiber diameter traits overcomes the above-mentioned deficiencies in the prior art, and it can effectively solve the existing process of breeding high-quality sheep with fine wool fiber diameter traits. , which has the problem of not being able to select early and quickly; the present invention discloses for the first time that the rs160836358 SNP site is applied to the selection of sheep wool fiber diameter traits

Method used

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  • Molecular marker related with diameter character of sheep wool fibers as well as specific primer and application thereof
  • Molecular marker related with diameter character of sheep wool fibers as well as specific primer and application thereof
  • Molecular marker related with diameter character of sheep wool fibers as well as specific primer and application thereof

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Embodiment 1

[0022] Embodiment 1: The molecular marker related to the trait of sheep wool fiber diameter is located at the 30th exon of FAT3 gene chromosome 21 (1481091), and the nucleotide sequence of the 30th exon is as shown in the sequence table SEQ ID No.1 is shown.

Embodiment 2

[0023] Example 2: The pair of specific primers used to amplify molecular markers related to sheep wool fiber diameter traits includes two allele-specific primers (ASP1 / ASP2), one locus-specific primer (LSP) and A specific targeting amplification primer (STA), the sequence of an allele-specific primer (ASP1) is shown in the sequence table SEQ ID No.2, and the sequence of the other allele-specific primer (ASP2) is shown in the sequence The list of SEQ ID No.3 shows the sequence of the loci-specific primer shown in the sequence table SEQ ID No.4, and the sequence of the specific targeting amplification primer is shown in the sequence table SEQ ID No.5.

Embodiment 3

[0024] Embodiment 3: Reagent or preparation or kit of the specific primer pair described in embodiment 2.

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Abstract

The invention relates to the technical field of animal molecular markers, in particular to a molecular marker related with a diameter character of sheep wool fibers as well as a specific primer and application thereof in selection of diameter character of Chinese Merino sheep wool fibers. The molecular marker related with the diameter character of the sheep wool fibers is located at the No.30 exonof the No.21 chromosome of FAT3 gene. The molecular marker has the advantages that the function of using rs425144268 SNP site as the molecular marker of the diameter of the sheep wool fibers is disclosed for the first time, and the function of applying the rs425144268 SNP site to the selection of the diameter character of the Chinese Merino sheep wool fibers is disclosed for the first time; whenthe excellent diameter character of the wool fibers is identified by the molecular marker related with the diameter character of the sheep wool fibers, the operation is simple, and the sheep with smaller fiber diameter can be screened in an assisting way; because the detection level of the molecular marker is started, the accuracy of variety selection is improved, and the detection efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of animal molecular markers, and relates to a molecular marker related to the trait of sheep wool fiber diameter and a pair of specific primers thereof. The application in preparation or kit, the application of the molecular marker in the selection of Chinese merino sheep wool fiber diameter traits. Background technique [0002] As the wool spinning industry at home and abroad continues to update the processing of wool and cloth art, the requirements for wool raw materials are more stringent, the product trend has been developing in the direction of lightness and softness, and a new milestone has appeared in the quality of wool in the world. The demand for superfine wool is gradually increasing. The wool yield and fiber fineness quality of fine-wool sheep are important indicators to determine its economic value, and the objective inspection of wool quality is getting more and more attention in wool sales, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 田可川黄锡霞赵冰茹田月珍付雪峰徐新明吴伟伟哈尼克孜张艳花石刚于丽娟何军敏杜建文阿米娜玛尔孜娅
Owner 新疆畜牧科学院畜牧研究所
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