Molecular marking method for predicting and identifying length of sheep wool

Abstract

The invention relates to a molecular marking method, in particular to a molecular marking method for predicting and identifying the length of sheep wool. The method comprises the steps as follows: 1), extracting sheep genome DNA, designing a primer, performing PCR (polymerase chain reaction) amplification, performing enzyme digestion, and obtaining an enzyme digestion product; 2), subjecting the enzyme digestion product to electrophoretic separation, and judging genotypes according to an electrophoretic separation result; 3), performing association analysis and estimating the least square mean value of characters, and obtaining a result that the length of wool of a GG genotype individual is remarkably higher than that of wool of AA genotype and AG genotype individuals in the three genotypes; and 4), constructing wool length character breeding population taking the GG genotype individual as the primary so that the length of the sheep wool is predicated and identified. According to the method, the operation is simple, the cost is low, the accuracy is high, and automatic detection can be performed. Selective breeding of the sheep wool length is performed with the molecular marking method, so that a genetic breeding process of the length characters of the sheep wool can be accelerated, breeding sheep can be early selected, the sheep can be selected and left after birth, and a sheep breeding process is accelerated.

Description

technical field [0001] The invention relates to a molecular marking method. Background technique [0002] Fine-wool sheep occupy an important position in my country's animal husbandry industry, and the main product of fine-wool sheep is wool. As an important textile raw material, fine wool has high economic value. The production of fine-wool sheep is not only related to the economic development and social stability of the producing areas, but also to the development of my country's wool spinning industry and the balance of import and export trade. With the increasing demand for wool at home and abroad, the cultivation of high-quality fine-wool sheep has become an urgent problem to be solved in the field of wool production and sheep breeding. [0003] The breeding of Chinese Merino sheep (Xinjiang military reclamation type) began in 1972, and has successively bred six strains, namely military reclamation type A strain, military reclamation type B strain, ultra-fine strain, ...

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Dkk1, Dkk2 and Dkk4 inhibit the Wnt signaling pathway by binding to Lrp6 and Kremen2, but the binding of Dkk2 and Dkk4 to Lrp6 and Kremen2 is weaker than that of Dkk1, while Dkk3 does not have this function

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