105results about How to "Speed up genetic progress" patented technology

An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.

Methods and cell lines for cloning bovine embryos and offspring are provided. The resultant embryos or offspring are especially useful for the expression of desired heterologous DNAs.

The invention discloses a crossing breeding and feeding method of wild boars, domestic pigs and hybrid pigs. The wild boars are not fit for feeding and popularization for the reasons that the number of the wild boars is limited, the meat is not fine and the skin is thick, and mouthfeel is worse. The wild boars are oestrous seasonably, produce fewer piglets, and are not easy to feed wild boars. However, the interest of people of pursuing venison and changing taste is increasing. The method for domesticating wild boars is a task that people pay attention to. The method includes: choosing long and white sows or big and white sows, carrying out crossing breeding through crossing generation I, backcrossing generation II, horizontal crossing generation I, and side crossing generation II of the sows and wild boars, total 6 generations. 12-16 months are required for one generation; or choosing duroc sows, carrying out crossing breeding through crossing generation I, backcrossing generation I, horizontal crossing generation II within lines, and horizontal crossing generation III among lines of the duroc sows and wild boars, total 7 generations, 12-14 months are required for each generation. The crossing breeding and feeding method of wild boars is used for crossing breeding and feeding.

The invention provides methodologies for improved molecular genetic analysis of individual animals and animal populations. The invention includes methods and systems for identifying those animals in a population that are most likely to heritably pass on desirable traits. Provided are means for evaluating the estimated breeding values and increasing the average genetic merit for animals in a population. For each trait, the instant invention provides methods for evaluating the relative effect of one or more quantitative trait loci (QTL) and three or more molecular genetic markers for each QTL The relationship between these various markers and the pre-selected trait and QTL is calculated, along with the contribution of other factors such as pedigree and known measures with respect to quantitative trait, and these data are used to calculate estimated breeding values for the animals in the herd and to rank the animals according to these estimated breeding values.

The invention belongs to the fields of molecular biological technology and molecular marker technology and relates to an SNP molecular marker related to intramuscular fat content of a Duroc boar. Thesite of the SNP marker is the 117427086th nucleotide site on the chromosome 7 of the International Pig Reference Genome 11.1 version, and the basic group of the site is G or A. By preferably selectingthe dominant allele of the SNP, the frequency of the dominant allele can be increased generation by generation, the intramuscular fat content of the boar can be increased, and the genetic improvementprogress of the boar can be accelerated, thereby effectively improving the economic benefit of boar breeding.

The invention discloses multi-character selection breeding method of fish and shrimps. The method comprises the following steps of: firstly obtaining individual selection indexes and family selection indexes according to measured values of breeding target characters, then reserving stocks and hybridizing according to the selection indexes, building family mating groups and finally obtaining bred populations of the next generation. Moreover, when the individual selection indexes are obtained, the stock reservation rate and the heritability of breeding target characters are firstly calculated according to the measured values of the breeding target characters, expected selection intensity is calculated according to the stock reservation rate, then an expected selection response and a corresponding weighted value of each breeding target character are obtained according to the expected selection intensity, the heritability and the standard difference of a standardized breeding value, and finally the individual selection indexes are obtained by using the weighted value. Therefore, the subjectivity and the randomness during calculation of the weighted value are eliminated, the reliability and the accuracy of the breeding method are high, and the comprehensive genetic progress of multi-character selection is improved.

The invention provides an SNP molecular marker related to egg weight and application of the SNP molecular marker. The SNP molecular marker corresponds to 169, 930, 974th deoxynucleotide of a Number one chromosome positive-sense strand of chicken reference genome Gallus_gallus-4.0 version sequence information issued in NCBI, wherein the nucleotide is T or G. An SNP locus is never reported before, thereby being a newly-found genetic molecular marker. The SNP molecular marker can be applied to early-stage selection of egg weight at different week ages and assisting of chicken breeding, lowering of production cost and accelerating of genetic progress can be facilitated, and the SNP molecular marker has quite high economic application value and scientific research value.

The invention discloses an aquatic animal selective breeding method adopting a group as a unit, and the method comprises the following steps of establishing a basic group adopting the group as a unit to form a current generation group; acquiring a group selective index; sorting a seed group and a seed individual; selecting pairing groups, establishing a mating group, and generating a next generation group; setting a virtual male parent and a virtual female parent for each next generation group; and repeating the steps to acquire the needed next generation group. By utilizing the selective breeding method, the technical difficulty that the breeding target trait test, genealogy establishment and orientation mating of aquatic animals of the individuals or family marks cannot be implemented can be effectively solved, the genetic evaluation of multiple breeding target traits and multiple generations can be realized, and the selection progress and the selection accuracy of the breeding target traits can be improved.

An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring. Production of genetically engineered or transgenic pig embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.

The invention discloses a stocking and mating method for a fish and shrimp group, comprising the following steps of: acquiring a selection index; sequentially selecting a certain amount of families from high family selection index to low family selection index as stocking families under a limited consanguinity coefficient; selecting male parent / female parent families and paired female parent / male parent families from the stocking family according to the sequence from the high family selection index to the low family selection index; and mating each male parent with corresponding female parent according to the sequence from high individual selection index to low individual selection index, creating male parent / female parent family mating groups, and form the next generation breeding group by all the mating groups. According to the stocking and mating method disclosed by the invention, a stocking and mating process of a breeding group is controlled according to the selection index and the consanguinity coefficient, and genetic progress of target character is effectively improved.

The invention discloses an SNP molecular marker related to chicken carcass traits and application thereof in breeding, wherein The SNP molecular marker corresponds to a mutation site in the gene sequence of the chicken let-7b precursor region. 526 G) A, and the genotype of the SNP site is AA, GA and GG. The SNP molecular marker of the present invention, that is, the mutation site in the gene sequence of the chicken let-7b precursor region g. 526 G)A is a new molecular marker associated with chicken carcass traits.. The early selection of carcass traits by determining the genotype of SNP locuscan save production cost and accelerate genetic progress. It can be used in chicken breeding and has great economic value and scientific research value.

The invention discloses a molecular marker capable of affecting the abdominal fat percentage of a chicken, and application of the molecular marker, particularly relates to application of a chicken ALDH1A3 gene 3' noncoding region sequence g.941 T>C molecular marker as a detection site for authenticating the abdominal fat percentage of the chicken, and is characterized in that the abdominal fat percentage of a CC genotype individual is obviously greater than the abdominal fat percentage of a TT genotype individual and the abdominal fat percentage of a TC genotype individual. When the molecularmarker disclosed by the invention used for carrying out marker assisted selection, the abdominal fat percentage obtained after the chicken is slaughtered can be greatly lowered, a feed use ratio of achicken flock is improved, and breeding cost is lowered. Through the molecular marker combination and a detection primer, an efficient and accurate molecular marker assisted breeding technology can beestablished, then, the efficient and accurate molecular marker assisted breeding technology is applied to abdominal fat percentage character genetic improvement after a breeding chicken is slaughtered, the abdominal fat percentage after the chicken is slaughtered is lowered, a feed use ratio of the chicken flock is improved, the sales of enterprises can be improved, and core competitiveness is increased.

The invention discloses an SNP molecular marker relevant to growth traits of a large white pig and application of the SNP molecular marker. The invention belongs to the molecular marker breeding technical field. The SNP molecular marker is located on the locus rs701332503 of the gene DHRS4 of the large white pig. The nucleotide sequence of the gene DHRS4 in the large white pig is relevant to the growth traits of the large white pig from the 5' end locus rs701332503, that is to say, the SNP molecular marker on the locus rs701332503 of the gene DHRS4 of the large white pig is relevant to the growth traits like the feed conversion ratio of the large white pig and is a new molecular marker, by determining the genotype of the SNP locus of the to-be-tested large white pig, the growth traits likethe feed conversion ratio of the large white pig are subjected to breeding pig sifting, the production cost can be effectively reduced, genetic progress can be accelerated, sifting of large white breeding pigs is better served, the huge market requirement can be met, and the SNP molecular marker also has high economic application value and scientific research value.

A SNP marker affecting pig daily gain traits and its application. The invention belongs to the field of molecular biotechnology and molecular marker technology, and particularly relates to the application of a porcine SNP molecular marker in the study of pig daily gain traits and pig breeding. The porcine SNP molecular marker site is shown in SEQ ID NO.1, located in the 321st nucleic acid single base mutation of the sequence fragment, named: g15588826C>T. The SNP molecular marker corresponds to the C>T mutation at the 15588826th nucleotide site on chromosome 3 of the International Porcine Reference Genome Version 10.2. By optimizing the dominant allele of the SNP molecular marker, the invention can increase the genetic progress of the daily gain trait of the Large White, reduce the breeding time for the daily gain trait of the Large White, thereby effectively improving the economic benefits of breeding pigs.

The invention provides an SNP (single nucleotide polymorphism) molecular marker combination for identifying the genetic relationship of Chinese Simmental. The marker combination contains 50 SNP markers distributed on 25 autosomes, wherein the average distance between adjacent SNP markers on the same autosome is 27M. The average values of the minor allele frequency (MAF), expected heterozygosity (HExp) and polymorphism information content (PIC) of the marker combination are 0.4818, 0.4998 and 0.3748 respectively; and when the genotype of the female parent is unknown, the accumulative exclusion probability is 0.9989. The SNP marker combination provided by the invention can be applied to complete and accurate pedigree identification of the Chinese Simmental, can improve the breeding accuracy of the Chinese Simmental and accelerate the genetic progress, and has good application prospect and economic benefit.

The invention belongs to technical fields of molecular biology technology and molecular markers, and specifically relates to a BMP6 gene as a molecular marker of a litter size trait of a black goat. An SNP molecular marker site of the goat is as shown in SEQ ID NO.1, the 179th nucleic acid of the sequence fragment undergoes single base mutation and is named T951C. The SNP molecular marker is corresponding to T greater than C mutation at the 951bp (calculated with the first base of a coding zone sequence as +1 site) site in the coding zone sequence of the BMP6 gene (accession number: AC_000180.1) on No.23 chromosome of bovine released in GeneBank. Further analysis finds that the mutation fails to cause the coded amino acid sequence to change and represents synonymous mutation. A dominant allele genotype of the SNP molecular marker is selected, thereby accelerating genetic progress of the litter size trait of the goat, shortening breeding time of a reproductive trait of the goat, lowering reproduction cost, and increasing economic profits of meat goat breeding industry effectively.

The invention belongs to the field of molecular biological techniques and molecular marker techniques, and particularly relates to a molecular marker on a pig chromosome No.7 related to the duroc pigdaily weight gain property and application of the molecular marker. An SNP site of the molecular marker on the pig chromosome No.7 related to the duroc pig daily weight gain property corresponds to the A>C mutation at the 27045611th-bp site on the international pig reference genome 11.1 version chromosome No.7, the molecular marker is obtained through whole-genome correlation analysis, the averagedaily weight gain property of 30-100 kg of a pig is obviously affected by the molecular marker. The invention further provides a primer pair for authenticating the molecular marker. By means of the molecular marker and the primer pair, the efficient and accurate molecular marker assisted breeding technology can be established; by applying the technology to the pig daily weight gain property genetic improvement, the daily weight gain of the pig is improved, enterprise benefits are improved, and core competitiveness is improved.

The invention discloses an SNP molecular marker related to shade of colors of green-eggshell egg shell of a chicken, and belongs to the technical field of molecular markers. An SNP locus of the SNP molecular marker is located at the position 64135653 on a chromosome 1 of the Primary Assembly version of an international chicken reference genome GRCg6a, that is, the base position 238898 of a gene PIK3C2G, and the base at the position is C or G. The invention further provides a primer combination for detecting the SNP marker and application of the primer combination. By using the SNP molecular marker and the primer combination, an efficient and accurate molecular marker assisted breeding technology of the green-eggshell egg shell color of the chickens can be established, so that a breeding method of green-eggshell egg shell color of the chickens is effectively improved, and the development of local characteristic laying hen industry is promoted.

The invention belongs to the poultry breeding technical field, and relates to a high production performance Rizhao ma chicken new strain cultivation method comprising the following steps: 1, using rizhao ma chicken having ma feather and green shank features to serve as male parent, using foreign broiler chicken as female parent, hybridizing to produce F1 generation, and reserving the hens having ma feather and green shank features in the F1 generation; 2, using the male parent in the step1 to serve as the male parent, hybridizing the male parent with the hens in the F1 generation so as to produce a F2 generation, selecting individuals having the ma feather and green shank features and same feather and appearance with the male parent in the step1, and keeping the selected chickens to breed and manage; 3, crossing and reproducing the male and female individuals obtained in the step2, and using a family and individual selection method to improve growing speed and egg laying capacity; 4, after breeding with beneficial characters for 4 or above 4 generations, the rizhao ma chicken new strain with foreign broiler chicken blood can be produced. The method can obviously improve early growing speed of rizhao ma chicken in our nation, can reduce feed conversion ratio, thus improving breeding performance in certain level.

The invention provides an SNP molecular marker related to reproductive characters of a Chinese Holstein cow and an application of the SNP molecular marker and belongs to the technical field of molecular breeding. A specific fragment is amplified from a PPARG gene of the cow, and a nucleotide sequence is as shown in a sequence table SEQ ID NO.1, wherein single-base mutation of G-T is formed at the 118th bp of the sequence table SEQ ID NO.1. The number of postpartum first breeding days, the number of nonpregnant days and the conception index of the Chinese Holstein cow employing G as a base on an SNP marker site are significantly lower than those of the Chinese Holstein cow employing T as the base. The invention further provides a primer for detecting the SNP marker and a kit containing the primer. The invention further provides an application of the SNP marker in identification of the dominant variety of the Chinese Holstein cow with high reproductive performance and an application in molecular marker-assisted breeding of the Chinese Holstein cow. The SNP molecular marker has the advantages of being simple in operation, high in sensitivity, high in accuracy and low in detection cost.

The invention discloses a big Duroc new strain breeding method, and belongs to the technical field of boar breeding. The big Duroc new strain breeding method includes: establishing a basic key group; allowing F0 generation Duroc boars to breed, and selecting 10 big Duroc male pigs having 7 blood relationships and big bodies and 60 female pigs to form a parent F1 generation; hybridizing the Duroc male pigs and the female pigs of the F1 generation, and selecting 10 big Duroc male pigs having 7 blood relationships and big bodies and 60 female pigs to form a parent F2 generation; and hybridizing the Duroc male pigs and the female pigs of the F2 generation, repeating the steps, selecting one generation each year, and breeding a big Duroc new strain after 3-4 generations. The breeding method can shorten the breeding generation interval, and improve the genetic progress; the big Duroc new strain has stable genetic performance and excellent production performance is acquired, and a product pig using the big Duroc new strain as a male parent is beautiful in body form, and is popular to markets; and the big Duroc new strain breeding method can meet the demand for high big fast-growth Duroc boars, and has very important significance.

The invention relates to a method for cultivating a new strain of recessive white and colored feather tiejiaoma broilers, and belongs to the technical field of poultry breeding, which comprises the following steps of: a, hybridizing to generate a F1 generation by taking parental CD of a broiler chicken from another country as a female parent and a tiejiaoma broiler with characteristics of black / green shins, jute feathers and white skins as a male parent, selecting and remaining F1-generation hens with the characteristics of the jute feathers and the black / green shins and F1-generation cocks with the characteristics of the jute feathers; and b, crossing male and female individuals selected and retained in the F1 generation athwart and propagating, grouping respectively according feather colors I, purifying and fixing beneficial characters according to a family and individual selection method, breeding by more than four beneficial characters for generations to cultivate new strains of four groups of broilers with jute feathers, recessive white feathers, black feathers and striped feathers. In the method, the high-productivity bloodlines of the broiler chickens from other countries is guided into the varieties of the tiejiaoma broilers successfully, the growth speed and egg-laying performance of the tiejiaoma broilers in China are improved substantially, and the genetic progress with large economic characters can be obtained within a short time.

The invention provides a porcine birth weight trait related SNP marker. The locus of the SNP marker is the 6083856th nucleotide locus on the chromosome No.12 of international pig reference genomes version 11.1, and the base of the locus is T or C. By optimizing preponderant alleles of SNP, the genetic progress of large white birth weight traits can be increased, and the breeding time of the largewhite birth weight traits can be reduced to effectively improve economic benefits of breeding pig breeding.

The invention discloses an SNP molecular marker related to a broiler chicken weight and shin length character, and application of the SNP molecular marker. The SNP molecular marker is positioned on the 135355388th nucleotide of the No.1 chromosome of a galGal6 version chicken genome, and corresponds to a mutation site g. 880C>T in a chicken TMEM182 gene sequence. The genotypes of the SNP molecular marker are CC, TCA and TT, wherein the weight of the CC genotype individual is greater than the weight of the TC and TT genotype individuals, and the shin length of the CC genotype individual is greater than the shin length of the TC and TT genotype individuals. The SNP molecular marker disclosed by the invention is characterized in that the mutation site g. 880C>T in a chicken TMEM182 gene intron 2 sequence is related to the weight and shin length character, the SNP molecular marker is a new molecular marker, early selection is carried out on the broiler chicken shin length character through determination of the genotype of the chicken SNP locus, production cost can be saved, and genetic progress is quickened.

The invention discloses an SNP molecular marker related to a cockscomb developmental character and a detection method thereof. A nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO:1, and the 302nd position of the SEQ ID NO:1 is G or A. The SNP molecular marker is related to the cockscomb developmental character and is a new molecular marker, by detecting the genotype of an SNP site of a to-be-detected chicken, early selection is conducted on the cockscomb developmental character, the production cost can be reduced, a genetic progress is accelerated, breeding of high-quality chickens can be better served, and the SNP molecular marker related to the cockscomb developmental character and the detection method thereof have a large economic value and scientific research value.

The invention relates to the technical field of gene engineering, in particular to an SNP molecular marker influencing the wool fiber diameter of alpine merino sheep and application of the SNP molecular marker. The SNP molecular marker influencing the wool fiber diameter of the alpine merino sheep is positioned on the 44288858th nucleotide locus A / G mutation on the seventh chromosome of the international sheep reference genome Oar_v4.0 version. The invention also provides a specific primer pair for identifying the SNP molecular marker and a kit containing the specific primer pair, and the specific primer pair and the kit can be used for early selection of a superfine strain of the alpine merino sheep to improve the seed selection accuracy, shorten the cultivation period and accelerate thebreeding process. The kit has the advantages of simple operation, can shorten the breeding time of excellent traits of the wool fiber diameter of the alpine merino sheep, reduce the breeding cost, shorten the cultivation period and accelerate the cultivation process, and has very high application value.

The invention belongs to the technical field of molecular biological and molecular marker technologies and especially relates to a multisite polymerization effect analytic breeding method for increasing lambing number of Hu sheep. BMPR-IB gene serves as a candidate gene that influences the character of the lambing number of Hu sheep, wherein there are three SNPs molecular markers, T-87C site, A746G site and G765A site, in the gene; by performing T-87C / A746G / G765A triple-site polymerization effect analysis to the SNPs molecular markers, it is found that CCGGGA is an optimum combined genotype. By means of the optimization of combined genotype, the genetic progress on the character of lambing number of Hu sheep is accelerated and early-stage selection efficiency of the Hu sheep is increased,thereby effectively increasing the economic benefit of mutton sheep breeding industry.

The invention discloses an SNP molecular marker related to a chicken pink eggshell color depth and belongs to the technical field of molecular markers. An SNP site of the SNP molecular marker is located at the 10544301th site of chromosome 20 of an international chicken reference genome GRCg6a Primary Assembly version, i.e. a 5561th base position of a gene PCIF1, and the base at the position is Cor T. The invention further provides a primer combination for detecting the SNP marker and application of the primer combination. By utilizing the SNP molecular marker and the primer combination, a molecular marker assistant breeding technology for an efficient and accurate chicken pink eggshell color can be established, so that a pink eggshell color breeding method is effectively improved, and development of local laying hen industry is promoted.

The invention discloses an SNP site combination and a method for predicting alive litter size genetic performance of to-be-tested pigs. The SNP site combination is composed of the 501st nucleotide from the 5'-terminal in the sequence 1, the 501st nucleotide from the 5'-terminal in the sequence 2, the 501st nucleotide from the 5'-terminal in the sequence 3, the 501st nucleotide from the 5'-terminal in the sequence 4 and the 501st nucleotide from the 5'-terminal in the sequence 5 in the genome of pigs. A test proves that prediction reliability value is 0.081 by means of the five SNP sites screened in the invention, which is increased by 13.8% than a BLUP method, so that by means of the five SNP sites, the genetic value of alive litter size of to-be-tested large white pigs can be predicted. The SNP site combination can accelerate genetic progress, brings economic benefit to breeding farmers, and has important application value.

The invention encompasses methods for increasing genetic progress in livestock, and for genetic dissemination, including the use of amniocentesis to obtain fetal amniocytes for use in genomic evaluation and cloning.