32results about How to "Improving Breeding Accuracy" patented technology

The invention provides a group of microsatellite markers for identifying parent-child relationships of Chinese Simmental cattle. The group of microsatellite markers includes ten microsatellite markers. The microsatellite markers have the advantages that the markers are high in polymorphism and are not linked with one another, fragments are appropriate in size and interval, and simultaneous detection can be facilitated; combined exclusion probabilities CPE (1), CPE (2) and CPE (3) can respectively reach 0.9960969, 0.999854 and 0.9999997 when the parent-child relationships of the Chinese Simmental cattle are identified by the aid of marker combinations under three different conditions, and accordingly the microsatellite markers are extremely high in detection efficiency; the microsatellite marker combinations can be used for completely and accurately identifying family trees of the Chinese Simmental cattle, accordingly, the Chinese Simmental cattle breeding accuracy can be improved, breeding procedures can be accelerated, and the microsatellite markers have excellent application prospects and economic benefits.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker influencing the wool shearing amount of alpine merino and application. The SNP molecular marker is located at the 20065540th nucleotide site G / A mutation on the fifth chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the shearing amount of the high-mountain merino sheep, shortening the breeding period and accelerating the breeding process, a high-mountain merino sheep shearing amount early selection technology is established, the breeding time of the excellent character of the shearing amount of the high-mountain merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

The invention discloses a composite PCR amplification system and an assay kit for genotyping of an STR mark detection system for individual identification and paternity test of yaks. The primer with afluorescence mark can achieve amplification of 15 STR mark sites of yaks, the mark sites are unlocked and have high heterozygosity and rich content of polymorphism information, and amplified productsare of right size. According to a detection method, genotyping results of 15 STR sites are obtained through amplification of 15 pairs of primers and electrophoresis detection; and individual identification and paternity test of yaks are carried out according to the STR locus genotyping results and gene frequency of each STR of yaks. Since individual identification and paternity test of yaks can be carried out effectively by comprehensively analyzing the STR locus genotyping results, accuracy of genealogy in yak breeding can be improved and the breeding process can be accelerated; accordingly,the composite PCR amplification system and the assay kit have good application prospect and economic value.

The invention provides an SNP (single nucleotide polymorphism) molecular marker combination for identifying the genetic relationship of Chinese Simmental. The marker combination contains 50 SNP markers distributed on 25 autosomes, wherein the average distance between adjacent SNP markers on the same autosome is 27M. The average values of the minor allele frequency (MAF), expected heterozygosity (HExp) and polymorphism information content (PIC) of the marker combination are 0.4818, 0.4998 and 0.3748 respectively; and when the genotype of the female parent is unknown, the accumulative exclusion probability is 0.9989. The SNP marker combination provided by the invention can be applied to complete and accurate pedigree identification of the Chinese Simmental, can improve the breeding accuracy of the Chinese Simmental and accelerate the genetic progress, and has good application prospect and economic benefit.

The invention relates to molecular genetics, and provides genetic markers for establishing and identifying a dairy cattle molecule system spectrum and application of the genetic markers. The genetic markers consist of 8 loci which are respectively TGLA227, TLA122, BMC1207, BM103, INRA037, INRA134, BP7 and MB026. The invention further provides primers of the 8 microsatellite loci, a method for obtaining fluorescence labeling primers by using the primers to perform fluorescence labeling capillary electrophoresis, a method for performing genotype judgment by using Gene Mapper V3.0 software together with Gene Marker software V1.75, a method for performing genetic relationship identification by using Cervus3.0 software, and a method for establishing an application kit. The genetic markers can be applied to dairy cattle system spectrum identification and establishment, are capable of making important basis for increasing the accuracy of dairy cattle species selection and breeding, are capable of promoting the breeding progress of dairy cattle, and have good application prospects and economical benefits.

The invention discloses an identification or prediction method of pork quality. The pork quality is identified and predicted by determining expression quantity of gene glycogenin-1-like and gene PRKAG3, the expression quantity of the gene glycogen synthase-1, the expression quantity of the gene hexokinase-1, and the expression quantity of the gene hexokinase-2 through real-time fluorescence quantification PCR (qRT-PCR). The method can be used for judging pork quality performance by detecting the expression quantity of the plurality of key genes, the process that the pig needs to be butchered to determine the pork quality is avoided, and the cost of pork quality performance detection is reduced. More reliable data can be added in the work of pig breeding through the formula, and the breeding accuracy is improved; and meanwhile partial compatriot determining is replaced, and the breeding cost of the enterprise is reduced.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP molecular marker influencing weaning weight character of merinos and application. The SNP molecular marker is located at the 3566348 nucleotide site T / C mutation on the 23<rd> chromosome of the international sheep reference genome Oar_v4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair, and a nucleotide polymorphism detection method. Compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the weaning weight characters of high-mountain merinos, shortening the breeding period and accelerating the breeding process, an early selection technology of the weaning weight characters of the high-mountain merinos is established, the breeding time of the excellent weaning weight characters of the high-mountain merinos is shortened, the breeding cost is reduced, and the application value is very high.

The invention relates to application of an anti-WSSV candidate molecular marker of the nLvALF1 gene of Litopenaeus vannamei to genetic breeding of Litopenaeus vannamei, belonging to the field of genetic breeding of aquatic livestock. Through preliminary study, three SNP markers (P<0.05) significantly correlated with anti-WSSV are screened out from the nLvALF1 gene by using a correlation analysis method. In the invention, resistance lines and non-resistance lines of the three SNP markers in other genetic breeding materials are subjected to SNP genotyping so as to eventually screen out one SNP marker, located at a site nLvALF1g-114-T>C, significantly correlated with anti-WSSV; the genotype frequency of the CC type of the site in resistance lines is substantially higher than the genotype frequency of the CC type of the site in non-resistance lines; and a method for molecular marker-aided genetic breeding of anti-WSSV is established by using the marker. The invention provides a candidate technical means for molecular marker-aided breeding for disease resistance and lays foundation for research on breeding of an anti-WSSV variety of Litopenaeus vannamei.

The invention discloses an anti-WSSV (white spot syndrome virus) candidate molecular marker from an anti-lipopolysaccharide factor gene (nLvALF6) of litopenaeus vannamei and application of the anti-WSSV candidate molecular marker. An SNP marker significantly correlated to anti-WSSV in the anti-lipopolysaccharide factor gene (nLvALF6) of the litopenaeus vannamei is an SNP locus (nLvALF6g-258-A>T) at the position 258bp in SEQIDnLvALF6-g. An effective marker is provided for vannamei disease-resistant molecule breeding, an important guidance is provided for application of the disease-resistant molecular marker to genetic breeding, and the anti-WSSV candidate molecular marker has very good application prospects in the study of vannamei anti-WSSV variety breeding.

The invention discloses a complete set of reagent and a method for identifying a parent-child relationship of Beijing ducks. The complete set of reagent for identifying or assisting to identify the parent-child relationship of the Beijing ducks is composed of a complete set of reagent 1, a complete set of reagent 2 and a complete set of reagent 3; the complete set of reagent 1 is prepared from IAS05-P, IAS07-P and IAS11-P; the complete set of reagent 2 is prepared from the complete set of reagent 1 and a reagent A; the reagent A is prepared from IAS06-P and IAS08-P; the complete set of reagent 3 is prepared from the complete set of reagent 2 and a reagent B; and the reagent B is prepared from IAS12-P, IAS13-P, IAS04-P, IAS03-P, IAS01-P and IAS14-P. Proved by experiments, the combined exclusion probability of the complete set of reagent of identifying the Beijing ducks can reach 0.99 or above, and the complete set of reagent can be used for complete and accurate pedigree identification and individual recognition of the Beijing ducks and can correct chaotic Beijing duck breeding pedigrees.

The invention relates to application of an rs81439242 SNP molecular marker in breeding of live pig varieties related to body length. The rs81439242 SNP marker integrates into Chinese local pigs from western pig consanguinity, is remarkably associated with the body length (P<0.01), and can be used for molecular marker-assisted selective breeding of Chinese local pigs related to body length, thereby accelerating breeding speed more effectively and improving breeding accuracy. The rs81439242 SNP marker has important economic benefits and social application values.

The invention provides an SNP molecular marker combination for establishing a Luxi cattle pedigree. The SNP molecular marker combination comprises 100 SNP markers distributed on 29 autosomes, the average distance of adjacent SNP markers on the same chromosome is 24M. Mean values of minor allele frequency (MAF), expected heterozygosity (HExp) and polymorphic information content (PIC) of the markercombination are respectively 0.4836, 0.4997 and 0.3751; and the accumulative probability of exclusion is 0.9991. The SNP marker combination can be used for completely and accurately identifying the pedigree of Luxi cattle, can improve the accuracy of Luxi cattle breeding and accelerating the genetic progress of the Luxi cattle, and has an excellent application prospect and economic benefit.

The invention discloses a compound PCR amplification system and a detection kit for genotyping of the STR marker detection system for paternity identification and individual identification of yaks. The primers have fluorescent markers and can amplify 14 STR marker sites of yaks. These markers are highly polymorphic, unlinked among the markers, and have appropriate fragment sizes; the STR detection system obtains the genotyping results of 14 STR loci through the amplification and electrophoresis detection of the 14 pairs of primers; The paternity test and individual identification of yaks were carried out based on the type results and the gene frequency of each STR in yaks. The scheme of the present invention can effectively perform paternity identification and individual identification of yaks by comprehensively analyzing the typing results of STR loci, thereby improving the accuracy of pedigree in yak breeding work, speeding up the breeding process, and has good application prospects and economic value.

The invention belongs to the technical field of molecular genetics, and in particular relates to a SNP molecular marker affecting the weaning weight traits of Alpine Merino sheep and its application. The SNP molecular marker is located at the T / C mutation at the 3566348th nucleotide site on chromosome 23 of the International Sheep Reference Genome Oar_v4.0 version. The present invention also relates to a specific primer pair for detecting the above-mentioned SNP molecular marker using PCR technology, a kit containing the primer pair and a detection method for nucleotide polymorphism. Compared with the traditional detection method, the technology has high accuracy and is It has the advantages of fast speed, low cost, and easy judgment of results. Using SNP locus detection for early selection of weaning heavy traits in alpine Merino sheep, shortening the breeding cycle and speeding up the breeding process, establishing an early selection technology for weaning heavy traits in alpine Merino sheep, reducing the weaning weight of alpine Merino sheep The breeding time of excellent traits reduces the breeding cost and has high application value.

The invention relates to a cultivating method of hyriopsis cumingii with a golden yellow shell. The method is characterized by comprising the following steps of (1), selecting 4-6 parent hyriopsis cumingii; (2) taking the margin tissues of the pallium of the parent hyriopsis cumingii, extracting the total RNA (ribonucleic acid) and synthetic single stranded c RNA, performing SRAP (sequence-related amplified polymorphism) amplification by adopting primer combination, and selecting male and female individuals with specific strip sequences with the golden yellow shells after the amplification products are subjected to electrophoresis; (3) using the selected male and female parent hyriopsis cumingii to build a family and cultivating in a water flowing pool; (4) periodically examining the pregnant condition of the female hyriopsis cumingii, and if mature glochidiums are found, selecting the mature female hyriopsis cumingii to reproduce to obtain infant hyriopsis cumingii; cultivating the infant hyriopsis cumingii in the water flowing pool until the shell reaches 1-2cm long, removing into to a net cage, and suspending the net cage into a pond to perform second stage cultivating on the infant hyriopsis cumingii until the shell length reaches 5-8cm; (5) removing the family parent hyriopsis cumingii with the non-golden yellow shells, and using the rest parent hyriopsis cumingii to build a core cultivating population with the golden yellow shells. According to the cultivation method of the hyriopsis cumingii with the golden yellow shell, the ratio of the hyriopsis cumingii with the golden yellow shell can reach more than 99.0%, the shell color is stable in heredity and the postoperative survival rate is high.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker for identifying the weaning weight of alpine merino and application of the SNP marker, and the SNP molecular marker is located at the 3566034th nucleotide site C / T mutation on the 23rd chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the weaning weight of the high-mountain merino sheep, shortening the breeding period and accelerating the breeding process, a technology for early selection of the weaning weight of the high-mountain merino sheep is established, the breeding time of excellent characters of the weaning weight of the high-mountain merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

The invention belongs to the technical field of livestock molecular biology, and provides a kit and method for family division and paternity identification of domestic pigs. The kit and method comprises the following steps: specifically selecting 14 SSR loci of domestic pigs, especially Anqing Liubai pigs, synthesizing corresponding primers for the loci, conducting capillary electrophoresis detection on ear tissue DNA of 98 Anqing Liubai pigs, counting and calculating the number of effective alleles, heterozygosity, polymorphic information content, genetic distance and exclusion rate of each locus, obtaining family division of the domestic pigs, especially the Anqing Liubai pigs, and performing paternity identification. The cumulative elimination probability of 14 microsatellite loci reaches 99%. The 14 microsatellite loci selected by the research have polymorphism in the Anqing Liubai pig population and can be used as effective genetic markers to be applied to production practices such as family division, paternity identification and the like of domestic pigs, especially the Anqing Liubai pig population.

The invention belongs to the field of poultry molecular breeding, and relates to a duck egg shell color molecular marker and its application in duck egg shell color prediction and egg-type breeding duck selection. The nucleotide sequence of the molecular marker of the present invention is as shown in SEQ ID NO.1 or SEQ ID NO.2, and the change of the genotype of the above-mentioned molecular marker G1157A site can be analyzed by PCR and sequencing methods, which can be accurate, fast and effective The eggshell color prediction of duck eggs provides a powerful molecular biological basis and implementation method for duck breeding.

The invention discloses a compound PCR amplification system and a detection kit for yak individual identification and paternity identification of the STR marker detection system genotyping. These markers are not linked, have high heterozygosity, rich polymorphic information content, and the size of the amplification product is appropriate; the detection method obtains the genotyping results of 15 STR loci through the amplification and electrophoresis detection of the 15 pairs of primers; According to the typing results of the STR loci and the gene frequency of each STR in the yak, the individual identification and paternity identification of the yak were carried out. The scheme of the present invention can effectively carry out individual identification and paternity identification of yaks by comprehensively analyzing the typing results of STR loci, thereby improving the accuracy of pedigree in yak breeding work, speeding up the breeding process, and has good application prospects and economic value.

The invention relates to a breeding method for extra-early-maturing sweet potato varieties, which belongs to the technical field of conventional cross-breeding. Sow hybrid seeds with substrate, and 45±5 days after sowing, the height of seedlings reaches 25±5 cm, investigate the tuber formation of seedlings, and select lines with an enlarged diameter of more than 0.8 cm as new lines of selected seed tubers, and then select and breed The new extra-early-maturing sweet potato strain of the present invention has high breeding accuracy, short breeding period and low breeding cost.

The invention belongs to the field of crop breeding, and particularly relates to a breeding method of winter wheat by a molecular marker-assisted selection generation-adding technology. The invention provides an efficient winter wheat breeding technology combining a modern biotechnology and a north-south generation adding technology, the breeding accuracy is improved by screening target genes, determining breeding targets and adopting molecular marker-assisted selection, and the breeding process is accelerated by north-south generation adding.

The invention relates to the technical field of gene engineering, in particular to an SNP molecular marker influencing birth weight of alpine merino and application of the SNP molecular marker. The SNP molecular marker is located at the 18805219th nucleotide site C > G mutation on the 14th chromosome of the international sheep reference genome Oar_v4.0 version. The invention also relates to a specific primer pair for detecting the SNP molecular marker by using an SNaPshot technology, a kit containing the specific primer pair and a nucleotide polymorphism detection method. Compared with a traditional detection method, the technology has the characteristics of high accuracy, high detection speed, low cost, easiness in result judgment and the like. The SNP locus detection is used for carrying out early selection of the birth weight character of the alpine merino sheep, shortening the breeding period and accelerating the breeding process, an early selection technology of the birth weight character of the alpine merino sheep is established, the breeding time of excellent characters of the birth weight character of the alpine merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

The invention relates to a western pig consanguinity infiltration site SNP marker related to the body length in Chinese local pigs, and an application of the SNP marker. The marker is at least one ofthe following mutations: the 2096738th base (rs81439116) of pig chromosome 12 of an international pig genome 11.1 version reference sequence is mutated from A to G, the 2129234th base (rs81439242) ismutated from C to T, and the 2145858th base (rs81439307) is mutated from T to C. The SNPs marker provided by the invention is infiltrated into the Chinese local pigs from the western pig consanguinity, is remarkably associated with the body length (P is less than 0.01), can be used for the molecular marker-assisted selective breeding of the body length of the Chinese local pigs, can more effectively accelerate the selective breeding speed and improve the selective breeding accuracy, and has important economic benefits and social values.

The invention discloses a chloride channel gene fragment related to intramuscular fat deposition of pigs and application thereof. The DNA fragment related to intramuscular fat deposition of pigs is shown in Sequence I of a Sequence Table. The invention also provides a method of applying the DNA fragment to detect the intramuscular fat deposition of pigs in an aided manner. The DNA fragment of the invention has a very obvious connection with the intramuscular fat deposition of pigs. Compared with the traditional methods, through the detection of pigs at birth by the method of the invention, the intramuscular fat deposition of pigs can be known at the earliest possible time, in addition, early selection can be realized and the breeding selection can be shortened, thus greatly reducing the expenses of the breeding selection. Meanwhile, in the method of the invention, a functional DNA fragment with effects is directly selected, thus improving the accuracy of the breeding selection and reducing the waste of manpower and materials. The invention has very great economic value and social significance.

The invention relates to a breeding method of a variety of extremely early-maturing sweet potatoes and belongs to the technical field of conventional hybridization breeding. The method comprises the steps of sowing hybrid seeds through a substrate, wherein the height of seedlings reaches 25+ / -5 cm 45+ / -5 days after sowing; investigating the potato setting condition of the seedlings; selecting a strain with the expanded diameter over 0.8 cm as a new strain of selected seedling potatoes; then, breeding the new strain of extremely early-maturing sweet potatoes. The new variety of the extremely early-maturing sweet potatoes has the advantages of high breeding accuracy rate, short breeding period and low breeding cost.