A method for the rapid artificial
inoculation in-vitro identification of Batryris elliptica Cocke resistance comprises the following steps: culturing Batryris elliptica Cocke in a culturing container by using a potato and oat
starch culturing medium, disinfecting by using an
autoclave, and standing for cooling to prepare a culturing medium; acquisition of lily to be measured: taking healthy initial-squaring-stage young lily plants which are not infected with Batryris elliptica Cocke pathogens and must have 6-9 healthy blades, and respectively taking complete lily blades from an upper position, a middle position and a lower position;
pathogen acquisition: taking typical infected lily blades, separating and purifying pathogens, identifying the pathogens as Batryris elliptica Cocke, culuring the pathogens on the potato and oat
starch culturing medium for 7d, and inoculating the healthy lily blades; inoculating by adopting a
mycelium block
inoculation process, a
spore spray
inoculation process or a mechanical inoculation process; wrapping petiole wounds of the
pathogen inoculated lily blades with wet cotton, putting the inoculated lily blades in a culture dish, and sprinkling a proper amount of
sterile water to carry out
moisture retention culture; and investigating the
disease infection conditions of the blades at the 3th day, the 5th day, the 7th and the 9th day, observing, and identifying. The Batryris elliptica Cocke resistance of different varieties of lily can be accurately and efficiently identified under controllable conditions through the method.