40 results about "Embryonic Stage" patented technology

The present invention pertains to a method for treating obesity in a mammal which comprises reducing the biological activity of HMGI genes in the mammal. In another embodiment, the invention pertains to a method for treating a tumor in a patient by reducing the biological activity of normal HMGI genes which comprises administering to the patient a therapeutically effective amount of an inhibitor compound active against normal HMGI-C or HMGI(Y) genes. In another embodiment, the invention pertains to a method of producing a transgenic non-human mammal, the germ cells and somatic cells of which contain an inactivated HMGI gene sequence introduced into the mammal at an embryonic stage. In another embodiment, the invention pertains to a method for screening candidate compounds capable of inhibiting the biological activity of normal HMGI proteins. In another embodiment, the invention pertains to a method for screening candidate compounds capable of inhibiting the biological activity of normal HMGI genes. In another embodiment, the invention pertains to a method for detecting normal HMGI proteins as a diagnostic marker for a tumor using a probe. that recognizes normal HMGI proteins, which comprises the steps of (a) contacting normal HMGI proteins from a sample from a patient with a probe which binds to HMGI proteins; and (b) analyzing for normal HMGI proteins by detecting levels of the probe bound to the normal HMGI proteins, wherein the presence of normal HMGI proteins in the sample is positive for a tumor. In another embodiment, the invention pertains to a method for detecting antibodies to normal HMGI proteins using a probe that recognizes antibodies to HMGI normal proteins, which comprises the steps of (a) treating a sample from a patient with a probe which binds to antibodies to normal HMGI proteins; and (b) analyzing for antibodies to HMGI proteins by detecting levels of the probe bound to the antibodies to HMGI proteins, wherein the presence of antibodies to normal HMGI proteins in the sample is positive for a tumor. In another embodiment, the invention pertains to HMGI genes and proteins for use as a starting point to isolate downstream target genes regulated by the HMGI genes and proteins.

Methods for making human ES cells and human differentiated cells and tissues for transplantation are described, whereby the cells and tissues are created following somatic cell nuclear transfer. The nuclear transfer donor is genetically modified prior to nuclear transfer such that cells of at least one developmental lineage are de-differentiated, i.e., unable to develop, thereby resolving the ethical dilemmas involved in reprogramming somatic cells back to the embryonic stage. The method concomitantly directs differentiation such that the desired cells and tissues may be more readily isolated.

The invention provides a special nutrient for grapes rich in organic selenium, which is prepared by evenly mixing nano selenium plant nutrient, organic phosphatic fertilizer and microbial fertilizer by weight ratio of 2-6:2-4:0.4-1. The invention also provides a method for producing grapes rich in organic selenium, comprising the following steps: in embryonic stage or flowering period of grapes, fertilizing the special nutrient for grapes rich in organic selenium according to an annular furrow application method with the total dosage of 45-150g of selenium per mu, namely, taking the root of the grape as the centre of a circle and furrowing, wherein, the furrow is 50-90 cm away from the root, the width of the furrow is 10-30cm and the depth is 10-20cm; fertilizing the special nutrient for grapes rich in organic selenium into furrows when the grapes are ripe; randomly checking the content of selenium of grape grains, and harvesting grapes with the content of selenium reaching 10-45 mu g / kg. The grape produced according to the method has higher security, and the selenium contained is easier to be absorbed and utilized by human body.

The invention relates to a male trait induction method for pelodiscus sinensis in embryonic periods. The male trait inducer is characterized in that through dissolving an aromatase inhibitor and an adjuvant into alcohol with the purity of 92-98%, the inducer with an aromatase inhibitor concentration of 50-150 mu g / mu l and an adjuvant concentration of 50-150 mu g / mu l is obtained; and the adjuvant is selected from testosterone, methyltestosterone or testosterone propionate. According to the induction method provided by the invention, the male induction rate can be more than 95% just through spraying the inducer two times at the sex determination key time point in embryonic development, and meanwhile, the normal development and low drug residues of pelodiscus sinensis gonads are ensured; and the induction method provided by the invention is easy to operate, low in cost, and remarkable in economic benefit, thereby being a sex control technology which is worthy of popularization in the existing pelodiscus sinensis breeding.

The invention relates to a positioning and marking method for embryonic-period primordial germ cells (PGCs), in particular to a bastard halibut embryonic-period primordial germ cell tracking and positioning method. The bastard halibut embryonic-period primordial germ cell tracking and positioning method includes the steps of fixing collected various periods of embryo samples of the bastard halibuts by a 4% PFA solution; using a PBS (phosphate buffer saline) solution with 50% of deionized formamide to preserve the embryo samples at the temperature of -20 DEG C, and subjecting the fixed and preserved embryo samples to oolemma removing, gradient methanol dewatering and rewatering; after rewatering, washing the various periods of embryo samples with PBS buffering liquid without RNA ( ribonucleic acid) enzyme, pre-hybridizing at the temperature of 62-65 DEG C for 2-4 hours; after hybridization, adding a hybridization solution with bastard halibut RNA probes into the various periods of embryo samples subjected to pre-hybridization for hybridizing overnight at the temperature of 62-65 DEG C; after hybridization, subjecting the various periods of embryo samples to washing, antibody incubation and rewashing, keeping away from light, and developing colors to achieve marking for tracking and positioning of the embryonic-period primordial germ cells of the bastard halibuts. The bastard halibut embryonic-period primordial germ cell tracking and positioning method has the advantages that the problems that yolks and oolemma of the samples hybridized in situ conventionally are difficult to strip and a background color is too deep after color developing detection are solved, and operation steps are simplified.

The present invention relates to an angiogenesis inhibitor comprising meteorin as an active ingredient that is highly expressed in astrocytes of the brain and retina in the late embryonic stage and after the birth of a mouse. It is in particular highly detected in astrocyte endfeet surrounding blood vessels and promotes the expression of thrombospondin-1 / -2 (TSP-1 / -2) via autocrine pathway and thus inhibits angiogenesis. The meteorin of the present invention can be effectively used for pharmaceutical compositions and health foods that prevent vascular diseases by inhibiting angiogenesis.

The invention discloses pelodiscus sinensis sex identification specific primers and a method. The two pairs of primers in a sex identification primer system are a pair of female specific primers and apair of positive reference primers which can be amplified by using male and female genomic DNA. Among the primers, the female specific primers can only stably amplify a 196 bp band in the female genomic DNA, and the band cannot be amplified in the male genomic DNA; the positive reference primers can amplify a 482 bp band in the male and female genomic DNA. Thus, in a PCR reaction system, the female genomic DNA can amplify two bands of 196 bp and 482 bp, while the male can only amplify a band of 482 bp. The use of the primers facilitates the correct identification of the sex of pelodiscus sinensis, especially the sex identification of the pelodiscus sinensis in the embryonic stage which is difficult to distinguish through naked eyes. The primers lay the foundation for the study of the pelodiscus sinensis, and also provide an important tool for the breeding and artificial breeding of the pelodiscus sinensis.

The invention discloses an SNP functional molecular marker of a lodging-resistant gene of japonica rice and application of the marker. The lodging-resistant gene is SCM2; the SNP functional molecular marker is K_06g_SCM2-1 and is positioned at the 27481645th basic group of the No. 6 chromosome of rice; the polymorphism of the SNP molecular marker is T / G; the sequence of the marker is shown in SEQ ID No.1; the base group at the 55bp site is T or G and is positioned in a promoter region of the gene SCM2. The SNP functional molecular marker disclosed by the invention can be used for predicting the lodging resistance of a japonica subspecies material of rice in an early stage (seedling stage or embryonic stage), and can be used for performing accurate screening, thereby promoting genetic improvement and breeding of lodging resistance traits of the japonica rice. According to the invention, a KASP method is adopted to detect an SNP site, and the detection method is accurate and reliable, easy and convenient to operate, and suitable for application in high-throughput commercialized molecular breeding.

The invention discloses a real-time long-range monitoring system for a drainage pump and a real-time long-range monitoring method of the real-time long-range monitoring system. The real-time long-range monitoring system for the drainage pump comprises sensors, protectors, a networking module and a data transmission terminal, which are sequentially connected, wherein the protectors and the networking module are connected through communication interfaces mounted on the protectors; the networking module and the data transmission terminal are connected through a network; the sensors are used for collecting real-time data parameters in the operating process of the drainage pump; the protectors are used for performing warning or cutting off the operation of the drainage pump when the drainage pump is abnormal; the networking module is used for networking real-time signals transmitted from the sensors of a plurality of drainage pumps; and the data transmission terminal is used for receiving transmission signals from the networking module. Compared with the prior art, the real-time long-range monitoring system for the drainage pump and the real-time long-range monitoring method of the real-time long-range monitoring system provided by the invention can effectively shorten the treatment time of management personnel and maintenance personnel, and the generation of failures is controlled at an embryonic stage.

The invention provides a change-prevention environment-friendly purification method and a change-prevention environment-friendly purification machine, which belongs to the disposal method and equipments of plate material, floors, and the like, in the technical field of decoration construction. The volatilization of matters such as formaldehyde, benzene, and the like, in the known plate material used for decoration can harm the health of people. Although the subsequent methods such as ventilation, adsorption, and the like, have a certain effects, the methods are measures which have to take after things occur and the prevention and control are not carried out before things happen. In order to overcome the environmental pollution of the plate material and realize the prevention and control in advance, the technical proposal adopted to solve the problem is that the anti-deformation disposal of the plate material under the conditions of pressure, heating, humidifying and wind power by a pressure device, a heating device, a humidifier and the conversion and reverse rotation of the wind speed of a fan can be realized; meanwhile, the wind power purification, heating purification, humidifying purification, cupping purification, high pressure purification and chemical purification process of the plate material acceleration are realized. The method and the machine of the invention have the beneficial effects of carrying out the anti-deformation disposal and environmental protection and purification disposal of the plate material in advance and avoiding the decoration plate pollution in embryonic stage.

The invention belongs to the technical field of animal breeding and particularly discloses a medicine composition for enhancing or improving a physiological function or production performance of sow breeding. The medicine composition comprises main components such as olive oil, squalene and wheat flour. The medicine composition disclosed by the invention is mainly used for improving the physiological function or sexual behavior, normal oestrus and breeding pregnancy rate of sow breeding, increasing the litter size, improving the nutrition environment at an embryonic stage, enhancing the immunity of piglets, and increasing the quantity of healthy piglets.

The invention provides a separation and primary culture method of pigeon craw fibroblasts. The method comprises the following steps of, taking pigeon craw tissues in an embryonic stage, cleaning, shearing the craw tissues into parts with volume of 1 mm < 3 >, uniformly spreading the craw tissues at the bottom of a cell culture dish, adding a small amount of FBS, putting into a cell culture box for6-8 hours, taking out, adding a complete culture medium, continuing culture, replacing the culture medium every 2-3 days, and culturing for 5-7 days to obtain primary cells with the convergence degree of 80-90%. The passage culture comprises the steps of, using PBS preheated to 37 DEG C in advance to clean the primary cells, adding trypsin for digestion, adding a complete culture medium when thecells shrink and turn round under a microscope, stopping digestion, using a pipette for gently blowing and beating the cells, and transferring the cells into a 15 ml centrifugal tube for centrifugation; re-adding the complete culture medium to re-suspend the cells, carrying out passage culture according to a ratio of 1:2, placing in a cell incubator for culture, and regularly replacing the culturemedium. According to the separation and primary culture method, the operation is simple, the obtained cells are large in quantity and high in purity, and subsequent stability culture can be realized.

The present invention provides for a transgenic nonhuman mammal whose germ or somatic cells contain a nucleic acid molecule which encodes calcineurin or a variant thereof under the control of a regulatable promoter, introduced into the mammal, or an ancestor thereof, at an embryonic stage. The present invention also provides for a method of evaluating whether a compound is effective in improving long-term memory in a subject suffering from impaired long-term memory which comprises: (a) administering the compound to the transgenic nonhuman mammal of claim 1 wherein the mammal has increased brain-specific calcineurin activity due to expression of the nucleic acid, and (b) comparing the long-term memory of the mammal in step (a) with the long-term memory of the mammal in the absence of the compound so as to determine whether the compound is effective in rescuing the long-term memory defect in the subject.

A method for detecting apoptosis of embryonic stages of parasitic helminthes. The method comprises isolating of intra uterine embryonic stages from an adult female parasite. The embryonic stages are cultured in vitro and treated. The said embryonic stages are subjected to flow cytometric analysis. An assay for apoptosis is performed being capable of high throughput screening and identification of compounds having apoptogenic activity towards the embryonic stages of helminthic parasites.

Provided here are methods for editing of a host genome to knock out or debilitate genes responsible for the growth and / or differentiation of a target organ and injecting that animal at an embryo stagewith donor stem cells to complement the missing genetic information for the growth and development of the organ. The result is a chimeric animal in which the complemented tissue (human / humanized organ) matches the genotype and phenotype of the donor. Such organs may be made in a single generation and the stem cell may be taken or generated from the patient's own body. As disclosed herein, it is possible to do so by simultaneously editing multiple genes in a cell or embryo creating a 'niche"' for the complemented tissue. Multiple genes can be targeted for editing using targeted nucleases and homology directed repair (HDR) templates in vertebrate cells or embryos.

As a result of screening for genes that are selectively expressed in fetal mouse brain region by subtraction method, the present inventors obtained a cDNA fragment encoding Corl1. The expression of Corl1 was examined by RT-PCR, in situ hybridization, and immunostaining using polyclonal antibodies. The results demonstrated that Corl1 was especially expressed at a high level of selectively in the central nervous system during embryonic stages. The expression patterns of Corl1 determined using various markers in embryonic spinal cord were compared to identify types of neurons expressing Corl1. The results revealed that Corl1 was specifically expressed in spinal cord interneurons dI4, dI5, dILA, and dILB. Accordingly, the present invention provides for discrimination between dI4 and dI6, neurons which previously could only be discriminated based on developmental location, using the expression of Corl1 as an indicator.

The invention relates to an embryonic stage nourishing agent capable of improving broiler chicken quality and promoting early stage growth, and applications thereof. According to the applications, athatching later stage, each egg is provided with 0.1ml of N-carbamyl-L-glutamic acid through amnion cavity using a 1ml vaccine syringe (0.25 to 6.0mg / each egg), so that newly hatched chicken quality isimproved, broiler chicken early stage growth effect is improved, weight loss caused by 48h of fasting for solids and liquids of newly hatched chicken is reduced obviously, body weight on 21 days is increased by 40 to 80g each chicken, and forage efficiency is increased obviously. The 100<mu>l injection amount and unsealing technology possess following advantages: hatching efficiency is not influenced, efficiency is high, operation is convenient, injection 800 to 1000 eggs can be realized by one person in each hour, the embryonic stage nourishing agent is suitable to be used at small hatcheries, and the economical benefit is excellent.

The present invention relates to a Pde6b-deficient animal model of retinal degeneration produced by engineered endonucleases, and a method for producing the same. In the animal model of retinal degeneration according to the present invention, only a specific target gene can be removed using engineered endonucleases, so that mutagenesis can be stably achieved. In addition, it is possible to produce a congenital animal model through genetic manipulation at the embryonic stage rather than through acquired factors, which allows for production of an animal model that uniformly exhibits symptoms of the disease in question without being influenced by other factors.

Genetically modified somatic cells of a non-human animal are provided that are engineered to contain a self-excisable, recombinase expression cassette comprising a site-specific recombinase gene operably linked to an ES cell-specific promoter. Compositions and methods for producing a genetically modified, cloned non-human animal that is free of a selective marker gene and a recombinase gene are provided, wherein a targeting construct comprising a self-excisable recombinase gene operably linked to an ES cell-specific promoter is introduced into differentiated somatic cells. The genetically modified genome of the somatic cells is transferred into an enucleated host oocyte. The artificially created zygote is then cultured in vitro until the blastocyst embryonic stage and subsequently implanted into a uterus of a surrogate mother to form a genetically modified, cloned non-human animal free of selective marker and recombinase genes.

The invention discloses pelodiscus sinensis sex identification specific primers and a method. The two pairs of primers in a sex identification primer system are a pair of female specific primers and apair of positive reference primers which can be amplified by using male and female genomic DNA. Among the primers, the female specific primers can only stably amplify a 196 bp band in the female genomic DNA, and the band cannot be amplified in the male genomic DNA; the positive reference primers can amplify a 482 bp band in the male and female genomic DNA. Thus, in a PCR reaction system, the female genomic DNA can amplify two bands of 196 bp and 482 bp, while the male can only amplify a band of 482 bp. The use of the primers facilitates the correct identification of the sex of pelodiscus sinensis, especially the sex identification of the pelodiscus sinensis in the embryonic stage which is difficult to distinguish through naked eyes. The primers lay the foundation for the study of the pelodiscus sinensis, and also provide an important tool for the breeding and artificial breeding of the pelodiscus sinensis.

Immunocompetent animal models having immunological tolerance to a xenograft and methods of producing the same. The animal models are tolerized to the xenograft in the pre-immune, fetal or embryonic stage, followed by transplanting the xenograft into the animal in the post-natal stage, such that the post-natal animal is immunologically tolerant to the xenograft, while remaining immunocompetent.

A transgenic non-human mammal whose germ cells and somatic cells contain a constructively activated tyrosine kinase receptor introduced into the mammal, or an ancestor of the mammal, at an embryonic stage. The transgenic non-human mammals can be used as ligand-independent in vivo models for the identification and development of selective tyrosine kinase modulators for the treatment of cancer.

The invention belongs to the technical field of animal breeding and particularly discloses a medicine composition for enhancing or improving a physiological function or production performance of sow breeding. The medicine composition comprises main components such as olive oil, squalene and wheat flour. The medicine composition disclosed by the invention is mainly used for improving the physiological function or sexual behavior, normal oestrus and breeding pregnancy rate of sow breeding, increasing the litter size, improving the nutrition environment at an embryonic stage, enhancing the immunity of piglets, and increasing the quantity of healthy piglets.

In one aspect, the invention provides a transgenic non-human animal model having germ cells and somatic cells containing an endogenous MMTV-SV40-Spy1A gene sequence introduced into said animal model or an ancestor of said animal model at an embryonic stage, wherein said gene sequence comprises a mouse mammary tumor virus gene (MMTV), a functionally disrupted SV40 gene (SV40) and a human Spy1A gene. In another aspect, the present invention provides a transgenic non-human animal model whose germ cells and somatic cells contain an endogenous Spy1A-pTRE-Tight gene sequence introduced into said animal model or an ancestor of said animal model at an embryonic stage. Preferably, the Spy1A-pTRE-Tight animal model expresses the Spy1A gene and develop cancer, preferably breast cancer, when administered with tetracycline, preferably doxycycline.

The present invention provides an artificial large-scale hatching method for the roe of the Tibetan bifidus cereus, the artificial large-scale hatching method, which is characterized in that the fertilized eggs are placed in the hatching frame for hatching in flowing water before the embryo formation period of the fertilized eggs ; After the fertilized eggs develop to the stage of embryonic formation, the dead eggs are removed, and the fertilized eggs are moved into the incubator to hatch. The hatching method has a high survival rate of hatched eggs and high production efficiency, and can be produced on a large scale in Tibet.

The invention provides an establishing method of a recoverable immortalized hepatic cell line. The establishing method comprises the following steps: separating a hepatic progenitor cell from liver in a mouse which is 12.5-14.5 days old in an embryonic period; guiding a retrovirus containing genes SV40T and HSV-TK into the hepatic progenitor cell; screening a monoclonal cell strain having a hepatic progenitor cell marker and having a function of differing to a mature hepatic cell; then guiding a retrovirus containing CD gene into the cell strain so as to obtain recoverable immortalized hepatic cell carrying double suicide genes. The cell strain can multiply in vitro to obtain the phenotype and functions of a normal hepatic cell, the safety of the immortalized cell becomes adjustable through controllable modes such as locus recombination and drug screening; when the cell is used in a human body, the biological safety of the immortalized cell is ensured to the greatest extent and the dangerousness of the immortalized cell is reduced; therefore, a reliable, safe and ideal hepatic cell material is provided for bioartificial liver technology.

The invention relates to a method for exploring the muscle cell formation time in an embryonic period. The muscle cell formation time is explored by utilizing technologies such as real-time fluorescent quantitative PCR (Polymerase Chain Reaction), flow cytometry, indirect immunofluorescence and the like, and the method is feasible and can be used for massively analyzing the proportion of muscle cells in a plurality of cells in an embryonic disc, and the time when the muscle cells form and expressing marked protein. The method has important guiding significance for researching embryonic disc development and muscle cell formation, establishing a muscle cell formation regulation and control network and the like, and greatly enriches the biological research of embryonic development. The method can be popularized to embryonic plates of different types of poultry in the embryonic period to analyze when various cells in the embryonic plates are formed, differentiated and developed, and a foundation is laid for researching overall embryonic development and decrypting genetic laws. The identification method provided by the invention is simpler, more sensitive and more efficient, can detect weak expression of genes and proteins in an embryonic period, can more truly reflect the formation condition of target cells, and can also be applied to other poultry researches.

The invention discloses an animal model construction method for transplanted vein restenosis research, which comprises the following steps of: selecting an F344RG rat with severe immunodeficiency caused by utilizing a CRISPR / Cas gene editing technology at an embryonic stage as an host, and taking the rest great saphenous vein blood vessels of a CABG postoperative patient as a graft; implanting human saphenous veins into the abdominal aorta of F344RG rats using a cannulation method to establish a humanized rat model. The establishment method of the model is simple, convenient and repeatable, the rat death rate is low, operation can be completed by only one person, and a feasible animal model scheme is provided for research on the aspect of CABG postoperative venous bridge restenosis.