The invention belongs to the technical field of cell culture, and in particular relates to a sorting method of hepatocytes of which embryonic hepatic cells are differentiated. According to the sorting method, the embryonic hepatic cells are cultured into embryoid bodies in a shaking or suspension culture mode, then the embryoid bodies are cultured in an adherent culture mode, so as to gradually grow and differentiate into round differentiated cell communities, and cells in a specific area are selected to be sorted in a specific period and are cultured again in the adherent culture mode according to spatial distribution regularities of the differentiated hepatic cells in the round cell communities. In the process of adherent culture, according to a cell differentiation situation, EGFs (Epidermal Growth Factor), TGFs (Transforming Growth Factor), HGFs (Hepatocyte Growth Factor), OSMs and the like are selected as hepatic growth factors in the process that the embryoid bodies are differentiated into the hepatocytes, so as to promote the hepatocytes to differentiate, the whole sorting process has the advantages of simplicity in operation and low cost, cellular damage can be obviously reduced, compared with the prior art, the hepatic cell differentiation rate is greatly increased, the sorted cells grow with good conditions, and the cell purity and the cell functional requirements of hepatocyte transplantation can be satisfied.