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Caspase-1-ES cell line induced by mifepristone

A technology of mifepristone and cell lines, which is applied in the field of cell lines, can solve the problems of reducing the tumor formation of ES cell transplantation, and achieve the effects of reducing the risk of tumor formation, maintaining curative effect, and improving safety

Inactive Publication Date: 2012-04-25
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the transfer of caspase-1 into ES cells, which not only maintains the efficacy of ES therapy, but also reduces the risk of ES cell transplantation into tumors.

Method used

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  • Caspase-1-ES cell line induced by mifepristone
  • Caspase-1-ES cell line induced by mifepristone
  • Caspase-1-ES cell line induced by mifepristone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Caspase-1 expression by mimetricone expresses the tumor formed by the formation of embryo stem cells

[0054] 1. Experimental purpose

[0055] Caspase-1 belongs to one of the inflammatory cysteension, which can cause inflammatory cell death after activation.Excessive expression in the body can also cause tumor cell death.After Caspase-1 activation, it can induce the firing membrane to form fine pores to enters the cells, causing permeability to dissolve; leading to the release of CASPASE-1 related cytokines such as IL-18 and IL-18;Unknown nucleotide cutase shear DNA.So we choose this gene as the killing gene.

[0056] By introducing the Geneswitch technology platform in ES cells, constructing a caspase-1 expression system for meterone-regulated, and observing the use of Mepitetanone treatment in vitro and in vivo situations in vitro and in vivo.The impact of tumor cells.

[0057] 2. Materials and methods

[0058] 1. The main reagents and materials

[0059] BSA, 5 ...

Embodiment 2

[0120] Example 2 Caspase-1 expression by meterone-1 expression clearing tumors formed by internal end brain embryo stem cells without removing transplanted dopamine neurons

[0121] 1. Experimental purpose

[0122] Study using Caspase-1-ES cells regulated by Mikfidone, tumor cells formed by ES cells are eliminated while reserving dopamine neurons formed by ES cell differentiation.

[0123] 2. Materials and methods

[0124] 1. The main reagents and materials

[0125] Rabbit Anti-OCT-4 antibodies (Cell Signaling Company), Rabbit Anticate Ainetic Anticidase (TH) antibodies, mouse antibody (CHEMICON Corporation), mouse antibody (ABCAM company), mouse anti-MAP2 antibody, Rat Anti-microtamuration-related protein 2 (MAP 2) antibodies (American SIGMA), goat anti-MAP2 antibody (Santa Cruz, Germany), Prolong Gold Antifade Reagent, Alpha-MEM culture solution, Alexa Fluor? 350 donkey antibody (Invitrogen, the United States), laser co -focusing microscope (LCM510) (German Zeiss), PA6 cell line...

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Abstract

The invention relates to a caspase-1-ES cell line induced by mifepristone. The collection number of the caspase-1-ES cell line is CCTCC No.C201193. The invention also provides a preparation method and application of the cell line. The invention has the advantages that: caspase-1 is transferred into an embryonic stem (ES) cell for the first time, and the differential potential of the cell is not influenced; the caspase-1 is highly expressed in the ES cell for the first time, it is proved that activated caspase-1 initiates DNA damage to cause death of undifferentiated caspase-1-ES cells in vitro, and a risk of nodule formation caused by ES cell transplantation is reduced; it is discovered that the highly expressed caspase-1 in the differentiated ES cell does not obviously influence the cell for the first time, and the treatment effect of the ES cell is maintained; and the safety of embryonic stem cell transplantation is improved, the effect of treating parkinson disease and other neurologic diseases is remained, and the caspase-1-ES cell line can be widely applied to stem cell transplantation.

Description

Technical field [0001] The invention involves a cell line. Specifically, it is a Caspase-1-ES cell line and its preparation methods and applications induced by Mepatone. Background technique [0002] Parkinson ’s Disease (PD) is the second common nervous system transgender disease in the second place. Its pathological characteristics are mainly manifested by the loss of black dopamine neurons and the formation of Louis small body.Commonly used treatment strategies are only limited to symptomatic treatment and minimize the clinical symptoms of patients. Such treatment methods cannot fundamentally reverse the occurrence and development of PD.Embryonic STEM Cells (ES cells) are cells with a variety of differentiation potential and can induce differentiation into specific cell types. This characteristic makes it have a broad prospect in cell replacement therapy.ES cell transplantation is expected to be applied to the treatment of PD, but a small amount of ES cells mixed with transpla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/85A61K35/54A61P1/16A61P3/10A61P9/00A61P9/10A61P19/04A61P19/08A61P21/04A61P25/00A61P25/14A61P25/16A61P29/00A61P35/02A61K35/545
CPCY02A50/30
Inventor 乐卫东王颐杨德华
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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