The invention relates to a calibration method for clearance between rolls of hot-rolling vertical rolls, which mainly solves the technical problem that strip steel is deficient in quality because verticality and alignment to calibration of vertical rolls are not considered in current methods. The technical scheme is that the calibration method for clearance between rolls of hot-rolling vertical rolls comprises the steps of: 1, finding a rolling center line and marking; 2, additionally arranging two position marks on a transverse slipway of a hot-rolling vertical roll device, and marking the distance from the marks to the rolling center line as |ZB|; 3, hitting apertures on both sides of the vertical rolls to marked positions, wherein a roll block is inside a marking line and aligned with the marking line; 4, measuring the distance from the lower ends on both sides of the vertical rolls to the rolling center line, and marking the measuring point as a point A, the radius of the rolls as R / 2 and the distances from A to a vertical roll operating side and from A to a transmission side as |OA| and |DA|; 5, comparing the measured value |OA|+R / 2 on the operating side with |ZB|, wherein if the difference is in a precision range, the verticality is good, and if the difference is not in the precision range, the clearance between rolls on the operating side is adjusted so that the difference is in the precision control range; 6, adjusting the clearance between rolls on the transmission side by the adjusting method in the step 5; and 7, measuring the total aperture of the vertical rolls, inputting the measured values and calibrating the clearance between rolls of vertical rolls after adjusting the verticality and alignment.