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Bifidobacterium cect 7765 and use thereof in the prevention and/or treatment of excess weight, obesity and related pathologies

A technology of CECT7765, bifidobacteria, applied in the field of bifidobacteria CECT7765 and its use in the prevention and/or treatment of overweight, obesity and related pathologies, capable of solving problems that cannot be attributed to bifidobacteria, etc.

Inactive Publication Date: 2014-03-05
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the studies in the above-mentioned patent documents did not use Bifidobacterium strains, but Lactobacillus or a combination of Lactobacillus and Bifidobacterium lactis Bb12, and modified use in diet, so these effects do not cannot be attributed to bifidobacteria (US20100111915; Luoto et al., 2010. Br J Nutr., 103(12): 1792-9; Luoto et al. 2010. Int J Obes (Lond). Mar16), and, these strains are different from this The applied strains are different

Method used

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  • Bifidobacterium cect 7765 and use thereof in the prevention and/or treatment of excess weight, obesity and related pathologies
  • Bifidobacterium cect 7765 and use thereof in the prevention and/or treatment of excess weight, obesity and related pathologies
  • Bifidobacterium cect 7765 and use thereof in the prevention and/or treatment of excess weight, obesity and related pathologies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0123] Example 1: Isolation and Identification of Pseudostrand Bifidobacterium CECT7765 Strain

[0124] B. pseudostrenobacterium strains were isolated from the feces of healthy breast-fed mice that had not been exposed to bifidobacterium-containing food or treated with antibiotics for at least one month prior to analysis. Samples were stored at 4°C and analyzed in less than two hours after collection. 2 g of samples were dissolved in 10 mM phosphate buffer containing NaCl (PBS) at a concentration of 130 mM, homogenized for 3 min using a Lab-Blender 400 shaker (stomacher) (Seward Medical, London, UK), and diluted in peptone water. Aliquots of 0.1 ml at different decimal dilutions were inoculated on MRS agar containing 0.05% cysteine ​​(Sigma, St. Louis, MO; MRS-C) and 80 μg / ml mupirocin (Man Rogose and Sharpe; Scharlau, Barcelona). After 48 hours of incubation at 37°C under anaerobic conditions (AnaeroGen, Oxoid, UK), isolated colonies were selected and their morphology det...

Embodiment 2

[0130] Example 2: Selection based on ability to modulate macrophage responses involved in low-grade chronic inflammation in vitro Bifidobacterium pseudostrandum CECT7765 strain

[0131] 2.1 Preparation of intestinal bacterial cultures and suspensions

[0132] The strain was inoculated in 10 ml of MRS fermentation broth (Scharlau Chemie S.A., Barcelona, ​​Spain) containing 0.05% of 1% cysteine ​​(MRS-C), cultivated for 24 hours, and then incubated at 37°C under anaerobic conditions (AnaeroGen ; Oxoid, Basingstoke, UK) for 22 hours. Cells were collected by centrifugation (6,000 g, 15 minutes), washed twice with PBS (10 mM sodium phosphate, 130 mM sodium chloride, pH 7.4), and then resuspended in PBS containing 20% ​​glycerol. Aliquots of these suspensions were frozen in liquid nitrogen and stored at -80°C. The number of viable cells after freeze-thaw was determined by culturing on MRSC agar plates for 48 hours. Viability was greater than 90% in all cases. Each aliquot ...

Embodiment 3

[0146] Example 3. Application of pseudostrand-shaped Bifidobacterium CECT7765 strain to the function of immune system cells, Immune and endocrine parameters of peripheral blood and central nervous system, as well as gut Microbiome Combination and inflammatory properties sexual influence

[0147] 3.1 Preparation of the culture of the object strain of the present invention

[0148] The CECT7765 strain was grown in MRS broth (Scharlab, SL-Barcelona, ​​Spain) supplemented with 0.05% (w / v) cysteine, and cultured at 37°C for 22 hours under anaerobic conditions (AnaeroGen; Oxoid, Basingstoke , UK). Cells were harvested by centrifugation (6,000 g, 15 min), washed with phosphate buffered saline (PBS, 10 mM sodium phosphate, 130 mM sodium chloride, pH 7.4), and resuspended in 10% skim milk. Freeze aliquots of the above suspension in liquid nitrogen and store at -80 °C until use. The number of viable cells was determined after 48 hours of incubation on MRS agar plates contain...

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Abstract

The invention relates to Bifidobacterium CECT 7765 and to the use thereof in the prevention and / or treatment of overweight, obesity and related pathologies. The invention likewise relates to the Bifidobacterium CECT7765 strain, to the cellular components, metabolites and secreted molecules thereof, to the combinations thereof with other microorganisms, and to compositions that include the preceding products, as well as to the use of one strain of the species Bifidobacterium pseudocatenulatumo and to the use of the CECT 7765 strain for preventing and / or treating obesity; excess weight; hyperglycemia; and diabetes, preferably type-2 Diabetes Mellitus; hepatic steatosis or fatty liver; dyslipidemia; metabolic syndrome; immune system dysfunction associated with obesity and excess weight; and an imbalance in the composition of the intestinal microbiota associated with obesity and excess weight.

Description

technical field [0001] The invention relates to the field of therapeutic activity of pharmaceutical compositions or formulations and the field of foods. In particular, the present invention relates to the strain Bifidobacterium pseudostrenobacterium CECT7765, its cellular components, metabolites, secreted molecules, combinations with other microorganisms, and compositions comprising the above, and also to the use of Bifidobacterium pseudostrenobacterium strains or using the CECT7765 strain for the prevention and / or treatment of obesity, overweight, hyperglycemia and diabetes, especially type 2 diabetes, hepatic steatosis or fatty liver, dyslipidemia, metabolic syndrome, immune system associated with obesity and overweight Dysfunctional, compositional imbalances in the gut microbiota associated with obesity and overweight. technical background [0002] Due to increasing prevalence and morbidity, overweight and obesity have become one of the major public health problems today...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P3/04A61P3/10A61P37/02A23L29/00A23L33/00A61K35/741A61K35/744
CPCC12N1/20A23L1/0345A61K35/744C12R1/01A61K35/741A23L33/135A61P1/16A61P3/00A61P3/04A61P31/00A61P31/04A61P3/06A61P37/02A61P3/10C12R2001/01C12N1/205A61K35/745A61K35/74A23V2400/535A23V2002/00
Inventor 约兰达·桑斯·埃兰斯约兰达·阿莱特·圣克鲁斯保拉·高芬
Owner CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
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