Method of quickly identifying whether living citrus are self-compatible

A citrus, fast technology, applied in the field of identification of citrus self-compatibility, can solve the problem of aniline blue sampling time staining and other times that have not been systematically reported, and achieve accurate and efficient identification

Inactive Publication Date: 2019-09-06
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional aniline blue dyeing method is mostly used in Solanaceae, Rosaceae and other crops, but in citrus species, the sampling time after pollination, the formula of tissue fixative, and the time of tissue softening and dyeing have not been systematically reported.

Method used

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  • Method of quickly identifying whether living citrus are self-compatible
  • Method of quickly identifying whether living citrus are self-compatible
  • Method of quickly identifying whether living citrus are self-compatible

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Embodiment Construction

[0030] The principles and features of the present invention will be described below, and the examples given are only used to explain the present invention, not to limit the scope of the present invention.

[0031] 1. Material selection

[0032] Select 8 citrus varieties as materials to be tested, as shown in Table 1.

[0033] Table 1 Eight citrus materials to be tested

[0034]

[0035] 2. Pollen collection

[0036] The flowers of the above 8 kinds of citrus materials are collected, the anthers are picked and collected on clean filter paper or white paper, and dried in an oven at 28°C for 12 hours to disperse the anthers and collect them in a centrifuge tube.

[0037] 3. Pollen vigor identification

[0038] Prepare the citrus plant pollen germination medium, as shown in Table 2, adjust the pH to 6.0 and not exceed 6.2. Add the culture medium to the cap of the 1.5ml centrifuge tube, and then evenly distribute 8 kinds of citrus pollen on the surface of the culture medium. Be careful not to...

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Abstract

A method of quickly identifying whether living citrus are self-compatible comprises the steps of 1, collecting citrus pollen; 2, preparing a citrus pollen germination medium, scattering pollen to themedium, and observing with a microscope whether activity of the pollen is normal; 3, dipping the pollen with normal activity, applying the pollen to stigmas, and covering each flower with a pollination bag; 4, over a certain period of time which is determined according to a citrus species, immobilizing the stigmas in an immobilizing liquid, washing with an ethanol solution, heating by a water bathafter washing to perform softening for a period of time which is determined according to the citrus species, washing the softened stigmas with clear water, and staining with a water-soluble aniline blue solution; and 5, observing germination condition of pollen on the stigmas with a microscope after staining so as to determine whether the citrus are self-compatible. A normalized process to identify whether citrus are self-compatible is provided herein; stigma acquisition time and softening time are provided for different citrus species after pollination; whether citrus plants are self-compatible can be identified efficiently and accurately.

Description

Technical field [0001] The invention relates to a method for identifying citrus self-compatibility, in particular to a method suitable for rapid identification of citrus self-compatibility in vivo. Background technique [0002] Citrus is the largest fruit tree in the world and the largest fruit tree in southern China, which has great industrial value. In citrus production, seedless fruit is a very important characteristic, which comes from self-incompatibility and parthenocarpy. Therefore, the study of self-incompatibility traits of citrus fruits has important value in production practice . [0003] Self-incompatibility is one of the most important mechanisms of angiosperms to prevent self-pollination, fertilization, and maintain species adaptability. This mechanism rejects or accepts pollen from individuals with similar genetic relationships by regulating stigma recognition, and ultimately achieves optimized offspring The purpose of the genotype. Self-incompatibility is genetic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 柴利军蒋靖东韦壮敏邓秀新
Owner HUAZHONG AGRI UNIV
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