A SNP molecular marker affecting weaning weight traits of Alpine Merino sheep and its application
A Merino sheep and molecular marker technology, applied in the field of molecular genetics, can solve the problems of slow genetic progress and long breeding cycle, and achieve the effects of high technical accuracy, low cost, and easy interpretation of results
Active Publication Date: 2022-02-08
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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Embodiment 1
[0033] Example 1 Correlation Between Different Genotypes of Alpine Merino Sheep and Weaning Weight
[0034] 1. Sample collection
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Abstract
The invention belongs to the technical field of molecular genetics, and in particular relates to a SNP molecular marker affecting the weaning weight traits of Alpine Merino sheep and its application. The SNP molecular marker is located at the T / C mutation at the 3566348th nucleotide site on chromosome 23 of the International Sheep Reference Genome Oar_v4.0 version. The present invention also relates to a specific primer pair for detecting the above-mentioned SNP molecular marker using PCR technology, a kit containing the primer pair and a detection method for nucleotide polymorphism. Compared with the traditional detection method, the technology has high accuracy and is It has the advantages of fast speed, low cost, and easy judgment of results. Using SNP locus detection for early selection of weaning heavy traits in alpine Merino sheep, shortening the breeding cycle and speeding up the breeding process, establishing an early selection technology for weaning heavy traits in alpine Merino sheep, reducing the weaning weight of alpine Merino sheep The breeding time of excellent traits reduces the breeding cost and has high application value.
Description
technical field [0001] The invention belongs to the technical field of molecular genetics, and in particular relates to a SNP molecular marker affecting the weaning weight traits of Alpine Merino sheep and its application. Background technique [0002] For breeding stock, fecundity is productivity, and reproductive performance is directly related to the economic effect of animal husbandry industry. The reproductive performance of sheep mainly includes indicators such as the total number of lambs born, the number of live lambs born, newborn weight, newborn litter weight, and weaning weight. The birth weight of sheep is the birth weight of sheep, which is an important quantitative trait to measure sheep, and affects the growth and development, production performance and carcass grade of sheep. Birth weight was also positively correlated with weaning survival rate, weaning weight and slaughter weight, and negatively correlated with slaughter age. Moreover, sheep with low birt...
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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 袁超郭婷婷卢曾奎刘建斌岳耀敬杨博辉牛春娥孙晓萍李建烨
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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