87results about How to "Easy interpretation of results" patented technology

The invention discloses a preparation method and an application method of a nucleic acid rapid detection kit for Bostaurus components in feeds and belongs to the field of molecular biology and immunology. According to the preparation method and the application method, a high-sensitivity and high-specificity method of a polymerase chain reaction in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunology detection; a unique primer is designed and the primer is labeled; an extracted target DNA is subjected to specific amplification and an amplified product is combined with a labeled antibody immobilized on a test strip in a developing solution to form a stable and visible detection strip and a quality control strip, so as to realize rapid and accurate detection on the bovine-derived components in food and feed.

The invention relates to a fluorescence quantitative PCR detection kit for beta-thalassemia mutation. The kit comprises a PCR mixing reaction liquid, a positive control and fluorescence probes for detecting beta-thalassemia mutation genotype, wherein the PCR mixing reaction liquid contains PCR primers for amplifying a gene fragment on which a mutation site is positioned, and the mutation site is at least one mutation site selected from deletion mutation of a base corresponding to the site 41/42 amino acid of beta-globin gene, C-to-T mutation of a base corresponding to the site 654 amino acid of the second intron of beta-globin gene, A-to-T mutation of a base corresponding to the site 17 amino acid of beta-globin gene, A-to-G mutation of a base corresponding to the site 28 amino acid on the upstream of the promoter of beta-globin gene, A base insertion mutation of a base corresponding to the site 71/72 amino acid of beta-globin gene, and G-to-C mutation of a base corresponding to the site 5 amino acid of the first intron of beta-globin gene. With the technical scheme of the present invention, rapid, accurate and high sensitivity detection of mutation conditions of beta-thalassemia gene can be achieved, and especially 6 special site mutations of beta-thalassemia gene can be detected, wherein the 6 special site mutations are common in Chinese.

The invention discloses a primer group, a kit and a method for detecting polymorphic sites of psychotropic drug related genes and relates to the technical field of gene polymorphism detection. The primer group comprises one or more of combinations 1-25. The primer group can be used for detecting the polymorphism of 25 SNP sites of 20 psychotropic drug related genes simultaneously and has the characteristics that the cost is low, result interpretation is simple, interference among SNP sites is avoided and the like.

The invention discloses a kit for dry-type fluorescent quantum dot joint detection diagnosis of pancreatic cancer and a preparation method of the kit. The kit can be used for joint detection of three tumor markers Ca19-9, CEA, CA242 for clinical diagnosis of pancreatic cancer on the same detection strip, monoclonal antibodies of CA19-9, CEA and CA242 are fixed on a nitrocellulose film by means of an immunochromatography technique for preparation of a reaction film, the three monoclonal antibodies are marked with quantum dots emitting different light, the antibodies marked with the quantum dots are mixed and sprayed on a glass fiber film for preparation of a conjugate pad, and the conjugate pad, a sample pad and a water absorbing pad are combined to form a quantum dot immunostrip. The kit is prepared with a double-antibody sandwich method, three antigens in whole blood or serum and plasma are quickly detected, numerous defects of existing independent detection methods are avoided, the three tumor markers can be detected simultaneously with one drop of blood, the result is more direct, the sensitivity and the specificity are both remarkably improved, and accordingly, the kit has great potential in clinical application.

The invention discloses a kit for detecting a novel-coronavirus-susceptive gene and a detection platform. The kit comprises primers shown in SEQ ID NO: 1 to SEQ ID NO: 38. The kit disclosed by the invention is high in sensitivity and high in specificity, human gDNA nucleic acid samples (about 5-6 gene copies) to the minimum of 0.01ng/microliter can be detected, and the consumption of samples can be lowered; 2-hole detection can be achieved through 35 loci, and the accuracy reaches 100%; through a repeated-optimized-completed pretreatment reagent, a variety of ingredients can be premixed, the difficulty of system preparing and operating by detection personal during clinical application is simplified greatly, operations are facilitated, and the threshold of use and the difficulty of beginning are effectively lowered; and through being matched with the integrated detection platform, the operation is simple, convenient and rapid, and results are detected and analyzed automatically.

The invention discloses a nucleic acid rapid detection kit for a geese origin component in food and feed and an application method of the kit, belonging to the fields of molecular biology and immunology. According to the invention, a high-sensitivity high-specificity method of PCR in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunological detection, extracted target DNA is subjected to specific amplification through designing a specific primer and labeling the primer and the amplified product is combined with a gold labeled antibody immobilized on the test strip in a developing liquid so as to form stable and visible detection zone and quality control zone, thereby realizing the rapid and accurate detection of geese origin component in food and feed.

The invention discloses a nucleic acid group suitable for detection of SNP sites of tumor drug-related genes and an application, and relates to the technical field of gene polymorphism detection. Thenucleic acid group comprises one or more of primer combination 1-primer combination 15. Polymorphism of 15 SNP sites of 9 tumor drug-related genes can be detected simultaneously by the nucleic acid group, and the nucleic acid group has the characteristics of low cost, simple result interpretation, no interference among SNP sites and the like.

The invention discloses a rapid nucleic acid test strip detection kit for a food-borne pathogenic microorganism cronobacter sakazakii and an application method of the kit, and belongs to the fields of molecular biology and immunology. According to the kit and the application method thereof, a high-sensitivity and high-specificity polymerase chain reaction method for nucleic acid detection is combined with an immune colloidal gold rapid-detection technology for immunological detection, unique primers are designed and labeled, extracted target DNA (deoxyribonucleic acid) is subjected to specific amplification, and an amplification product is bound with a gold labeled antibody fixed on a test strip in a developing solution to form a stable and visible detection strip and a stable and visible quality control strip, so that main food-borne pathogenic microorganisms are rapidly and accurately detected.

The present invention discloses a preparation method of a rapid nucleic acid detection kit for a donkey (Equusafricanusasinus) component in food and feed, and an application method thereof, and belongs to the field of molecular biology and immunology. According to the invention, the high sensitivity and high specificity polymerase chain reaction method in nucleic acid detection and the immune colloidal gold rapid detection technology in immunology detection are combined, the unique primers are designed, the primers are labeled, specific amplification is performed on the extracted target DNA, and the amplified product is bound with gold-labeled antibody immobilized on test paper strip in a spreading solution so as to form the stable and visible detection zone and the quality control zone, such that the rapid and accurate detection on the donkey source component in food and feed is achieved.

The invention discloses a reverse transcription loop-mediated isothermal amplification (RT-LAMP) kit for detecting pestedes petits ruminants viruses (PPRVs). The kit comprises an RT-LAMP primer, a 2X reaction buffer solution, an enzyme mixture (EM), a fluorescent visual detection reagent, ultrapure water and a reaction template, wherein the RT-LAMP primer includes outer primers F3 and B3, inner primers FIP and BIP and loop primers LF and LB. The detection kit is mainly used for detecting whether the PPRVs exist in lesion tissues suspiciously infected with the PPRVs. Specific detection and sensitivity detection prove that the RT-LAMP kit provided by the invention can monitor a reaction in real time and quantitatively detect a copy number of the PPRVs, a detection result is obtained quickly and accurately, and the convenience is brought for simply and quickly detecting the PPRVs.

This invention belongs to the moleculal biology field. It involves a kind of fluorescent quantitive PCR test kit of detection gene mutations. The test kit includes fluorescent quantitive PCR premixed liquid, fluorescent quantitive reaction liquid A, luorescent quantitive reaction liquid B (the feature is that it contains primer and anti-primer and fluorescence probes), positive comparison sample A, positive comparison sample B, positive comparison sample C. This invention features that: short detection time, easy operation, low quality requirement of sample DNA, the result reading is clear, direct with high sensitivity.

The present invention discloses a preparation method of a rapid nucleic acid detection kit for a duck (scientific name: Anas platyrhynchos) component in food and feed, and an application method thereof, and belongs to the field of molecular biology and immunology. According to the invention, the high sensitivity and high specificity polymerase chain reaction method in nucleic acid detection and the immune colloidal gold rapid detection technology in immunology detection are combined, the unique primers are designed, the primers are labeled, specific amplification is performed on the extracted target DNA, and the amplified product is bound with gold-labeled antibody immobilized on test paper strip in a spreading solution so as to form the stable and visible detection zone and the quality control zone, such that the rapid and accurate detection on the duck source component in food and feed is achieved.