87results about How to "Easy interpretation of results" patented technology

The invention relates to a method and a device for rapidly and quantitatively detecting a tumor marker with an immunochromatography test strip marked by quantum dots. The method comprises the following steps of: replacing collaurum particles by using quantum dots as a signal marker, coupling with an antibody corresponding to the tumor marker to be tested, and spraying or directly coating on a conjugate releasing pad, wherein the antibody on another site corresponding to the tumor marker and a second antibody are coated on a nitrocellulose membrane to form a T line and a C line respectively; assembling a sample pad, a combining pad, a reaction membrane and a water absorbing pad according to a certain sequence to prepare the immunochromatography test strip; detecting qualitatively and quantitatively according to fluorescent stripes presented on the T line and the C line and the strength of fluorescence; and providing a quantum dot fluorescence immune detection device. The detection device is simple, the operation is simple and rapid, the time consumption for detection is short, and the result judgment is easy. The method and device are particularly suitable for early-stage screening, diagnosis, judgment prediction and prognosis of the tumor markers in households, communities, hospitals and the like, and evaluation of treatment effect and followed observation of high risk groups.

The invention relates to an EML4-ALK (echinoderm microtubule associated protein like4-anaplastic lymphoma kinase) fusion gene fluorescent quantitative PCR (polymerase chain reaction) assay kit, which is applicable to assay of EML4-ALK fusion gene mutation in lung adenocarcinoma. The kit comprises probes, primers and positive controls, which are specially designed for conserved sequences of 9 fusion variations of the EML4-ALK fusion gene. The kit can be used for quickly and accurately assaying 9 most common EML4-ALK fusion gene variations with high sensitivity, namely 9 fusion variations of 7 variant subtypes, which are subtype 1 (E13; A20), subtype 2 (E20; A20), subtype 3 (E6a / b; A20), subtype 4 (E14; A20), subtype 5 (E2a / b; A20), subtype 6 (E18; A20) and subtype 7 (E14; A20), so that a real-time fluorescent quantitative PCR system for assaying 9 most common EML4-ALK fusion gene variations can be established.

The invention discloses a method for detecting nucleic acid by a colloidal gold chromatography technology and a reagent kit, and belongs to the technical field of medical biology. A universal test paper strip for nucleic acid detection is provided; colloidal gold particles are marked on a universal probe 1, and then, the universal probe is fixed on a glass cellulose membrane; the probe sequence is designed into a universal sequence; the test paper strip is fixed on a PVC (polyvinyl chloride) bottom plate; a sample pad, glass fiber, an NC membrane and water absorption paper are in sequential arrangement from the left side to the right side, wherein a T line (detection line) and a C line (quality control line) are arranged on the NC membrane; antibodies to labels b cover the detection line; antibodies to labels a cover the quality control line; the specificity probe A series and the specificity B series successfully combine a gold mark probe and nucleic acid amplification fragments in series; the specificity detection of nucleic acid amplification fragments is realized. The method has the advantages that the technical requirement on the experiment personnel is low; the required detection time is short; special instrument equipment is not needed; the popularization to basic levels and remote rural area medical institutions is easy.

The invention provides a K-Ras gene mutation typing fluorescence quantitative PCR detection kit which comprises PCR mixed reaction solution, a peptide nucleic acid probe, a mutant type K-Ras-AllgloTM fluorescent probe and a positive control sample. The invention further provides a K-Ras gene mutation typing fluorescence quantitative PCR detection method which comprises the following steps: firstly designing a pair of PCR primers and the peptide nucleic acid probe according to K-Ras genes, respectively designing the AllgloTM fluorescent probe against K-Ras gene mutation sites, and adding the PCR reaction solution, the PCR primers, the peptide nucleic acid probe and the AllgloTM fluorescent probe in a reaction system for carrying out fluorescence quantitative PCR detection. The method adopts the PNA technology and is combined with the technology of the AllgloTM fluorescent probe, thereby being capable of quickly and accurately detecting the K-Ras gene mutation sites in various cancer tissues with high sensitivity, and having short time, simple operation and clear, intuitive and safe judgment and reading.

The invention discloses a preparation method and an application method of a nucleic acid rapid detection kit for Bostaurus components in feeds and belongs to the field of molecular biology and immunology. According to the preparation method and the application method, a high-sensitivity and high-specificity method of a polymerase chain reaction in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunology detection; a unique primer is designed and the primer is labeled; an extracted target DNA is subjected to specific amplification and an amplified product is combined with a labeled antibody immobilized on a test strip in a developing solution to form a stable and visible detection strip and a quality control strip, so as to realize rapid and accurate detection on the bovine-derived components in food and feed.

The invention discloses a preparation method of a rapid nucleic acid detection kit for detecting canis familiaris components in feed and an application method thereof, and belongs to the fields of molecular biology and immunology. According to the invention, a high sensitivity and high specificity method of polymerase chain reaction in nucleic acid detection and an immuno gold staining rapid detection technology in an immunological detection are combined, a specific primer is designed and labeled, the extracted target DNA is subjected to specific amplification, and amplified product is combined with a gold labeled antibody immobilized on a test paper tape, so as to form a stable and visible detection band and quality control band, thereby achieving the rapid and correct detection of the canis familiaris components in the main feed.

The invention relates to the technical field of in-vitro nucleic acid detection and particularly provides a primer group and kit for detecting polymorphic sites of aspirin-resistant related genes, andan application thereof. The primer group and the kit for detecting polymorphic sites of aspirin-resistant related genes and the kit can detect polymorphism of genes such as COX1, COX2, GPIIIa, PEAR1,P2Y1, GPIa and PAI-1; the sensitivity is high, so that genome DNA low to 0.1ng / muL can be accurately detected; the specificity is good, the genome DNA up to 300ng / muL can not generate non-specific amplification; the whole Fluorescence PCR detection process can be finished only within 90 minutes, and the detection result is intuitive and easy in interpretation.

The invention relates to a fluorescence quantitative PCR detection kit for beta-thalassemia mutation. The kit comprises a PCR mixing reaction liquid, a positive control and fluorescence probes for detecting beta-thalassemia mutation genotype, wherein the PCR mixing reaction liquid contains PCR primers for amplifying a gene fragment on which a mutation site is positioned, and the mutation site is at least one mutation site selected from deletion mutation of a base corresponding to the site 41 / 42 amino acid of beta-globin gene, C-to-T mutation of a base corresponding to the site 654 amino acid of the second intron of beta-globin gene, A-to-T mutation of a base corresponding to the site 17 amino acid of beta-globin gene, A-to-G mutation of a base corresponding to the site 28 amino acid on the upstream of the promoter of beta-globin gene, A base insertion mutation of a base corresponding to the site 71 / 72 amino acid of beta-globin gene, and G-to-C mutation of a base corresponding to the site 5 amino acid of the first intron of beta-globin gene. With the technical scheme of the present invention, rapid, accurate and high sensitivity detection of mutation conditions of beta-thalassemia gene can be achieved, and especially 6 special site mutations of beta-thalassemia gene can be detected, wherein the 6 special site mutations are common in Chinese.

The invention belongs to the field of genetic gene detection, and in particular relates to a kit and a method for detecting the gene polymorphism of human MTHFR (methylene tetrahydrofolate reductase) based on a Taqman-MGB (Minor Groove Binder) probe. By adopting the kit and method, two SNP (single nucleotide polymorphism) sites namely C677t and A1298C can be detected at the same time, and the results are easy to judge and read. The MGB probe has a nonluminous 3'-end quenching group and a relatively low background, is more sensitive to detection of a single base mutation, and can judge and read different genotypes only according to a Ct value, and the result can be judged and read more easily compared with that of conventional methods of Taqman probe detection and HRM (high resolution melting) detection. The probe price is relatively low, the detection cost is relatively low, a polymorphism site only needs one-tube qPCR (quantitative polymerase chain reaction) detection, and the operation is simple. Moreover, the method disclosed by the invention does not need subsequent analysis of PCR products, so that while the detection cost is saved, the detection circle is greatly shortened, the detection efficiency is improved and the risk of false positive caused by PCR product pollution is reduced.

The invention discloses a primer group, a kit and a method for detecting polymorphic sites of psychotropic drug related genes and relates to the technical field of gene polymorphism detection. The primer group comprises one or more of combinations 1-25. The primer group can be used for detecting the polymorphism of 25 SNP sites of 20 psychotropic drug related genes simultaneously and has the characteristics that the cost is low, result interpretation is simple, interference among SNP sites is avoided and the like.

The invention discloses a LAMP (loop-mediated isothermal amplification) kit for detection of mycoplasma pneumoniae and a special LAMP primer for detection of mycoplasma pneumoniae. The special LAMP primer for detection of mycoplasma pneumoniae is designed according to a specificity conservative target sequence of a mycoplasma pneumoniae P1 gene (GenBank number: CP002077.1). The LAMP primer is formed by six primers including outer primers MP-16F3 and MP-16B3, inner primers MP-16FIP and MP-16BIP and loop primers MP-16LF and MP-16LB. By the aid of the LAMP kit and the special LAMP primer for detection of mycoplasma pneumoniae, quickness, convenience, high efficiency, high specificity and high sensitivity in qualitative detection of the mycoplasma pneumoniae in samples of pure bacteria, sputum, bronchoalveolar lavage fluid, throat swabs and the like can be realized without complicated instruments, and a new technical platform is provided for detection of the mycoplasma pneumoniae.

The invention discloses a kit for dry-type fluorescent quantum dot joint detection diagnosis of pancreatic cancer and a preparation method of the kit. The kit can be used for joint detection of three tumor markers Ca19-9, CEA, CA242 for clinical diagnosis of pancreatic cancer on the same detection strip, monoclonal antibodies of CA19-9, CEA and CA242 are fixed on a nitrocellulose film by means of an immunochromatography technique for preparation of a reaction film, the three monoclonal antibodies are marked with quantum dots emitting different light, the antibodies marked with the quantum dots are mixed and sprayed on a glass fiber film for preparation of a conjugate pad, and the conjugate pad, a sample pad and a water absorbing pad are combined to form a quantum dot immunostrip. The kit is prepared with a double-antibody sandwich method, three antigens in whole blood or serum and plasma are quickly detected, numerous defects of existing independent detection methods are avoided, the three tumor markers can be detected simultaneously with one drop of blood, the result is more direct, the sensitivity and the specificity are both remarkably improved, and accordingly, the kit has great potential in clinical application.

The invention relates to a method for rapid fluorescence detection of polynucleotide target objects simultaneously at room temperature and constant temperature. The method comprises the steps that at the constant temperature, single-chain binding proteins partially open parental chains of double chains of multiple templates to form single chains; recombinases are bonded with multiple pairs of primers to form complexes which are bonded to the parental chains under the action of accessory proteins, and multiple fluorescence probes are also bonded with a complementation region; DNA polymerases are bonded to the 3' terminals of the primers so as to perform subchain extension; exonucleases recognize tetrahydrofuran sites on the multiple fluorescence probes which are under the double chain condition, so that fluorophores and quenching groups are separated after digestion, and fluorescence is released; after the multiple fluorescence probes are cut, 3'-OH ends which are originally closed due to modification of the 3' terminals of the probes are exposed, and the DNA polymerases can further extend to form subchains; to-be-tested samples can be subjected to qualitative and semi-quantitative determination through detecting the shape of amplification curves and the strength of fluorescence signals. The method disclosed by the invention can be used for qualitative and semi-quantitative determination of multiple target objects simultaneously at room temperature and constant temperature.

The invention discloses a primer sequence and a test kit for gene detection of safe medication for children, belonging to the technical field of molecular diagnosis. The test kit comprises 22 pairs ofamplification primers and can specifically amplify 22 common locus regions of the safe medication gene for children; the 22 single-base extension primers are used for detecting genotypes of 22 loci of the safe medication gene for children; in addition, the test kit comprises a special reagent for pretreatment and detection. The test kit provided by the invention can realize one-hole detection ofdifferent genotypes of 22 common loci related to clinical safe medication genes for the children, and has the advantages of high sensitivity, strong specificity, high accuracy, simple and convenient operation, low cost, high throughput, rapid detection, automatic result interpretation and easy clinical popularization and application. The test kit provided by the invention can be applied to gene detection of safe medication for the children, provides a reliable detection system and test kit for safe medication for the children, and has important clinical application value and good market application prospect.

The invention discloses a kit for detecting a novel-coronavirus-susceptive gene and a detection platform. The kit comprises primers shown in SEQ ID NO: 1 to SEQ ID NO: 38. The kit disclosed by the invention is high in sensitivity and high in specificity, human gDNA nucleic acid samples (about 5-6 gene copies) to the minimum of 0.01ng / microliter can be detected, and the consumption of samples can be lowered; 2-hole detection can be achieved through 35 loci, and the accuracy reaches 100%; through a repeated-optimized-completed pretreatment reagent, a variety of ingredients can be premixed, the difficulty of system preparing and operating by detection personal during clinical application is simplified greatly, operations are facilitated, and the threshold of use and the difficulty of beginning are effectively lowered; and through being matched with the integrated detection platform, the operation is simple, convenient and rapid, and results are detected and analyzed automatically.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker influencing the wool shearing amount of alpine merino and application. The SNP molecular marker is located at the 20065540th nucleotide site G / A mutation on the fifth chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the shearing amount of the high-mountain merino sheep, shortening the breeding period and accelerating the breeding process, a high-mountain merino sheep shearing amount early selection technology is established, the breeding time of the excellent character of the shearing amount of the high-mountain merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

The invention discloses a primer combination sequence and kit for detecting child safe medication-related gene mutation sites. The kit includes 23 pairs of amplification primers and 23 single-base extension primers; the 23 pairs of amplification primers can specifically amplify the 23 common mutation site regions of child safe medication-related genes, and the 23 single-base extension primers areused to detect the 23 mutation site genotypes of the child safe medication-related genes; and the kit further includes special reagents for pretreatment and detection. The kit provided by the invention can realize one-hole detection of the 23 mutations related to the clinical child safe medication-related genes, has high sensitivity, strong specificity and high accuracy, and is simple to operate,low in cost and high in throughput, the detection is fast, automatic interpretation of results is realized, and the clinical promotion and application are easy to realize; and the kit can be applied to the detection of the genes related to safe medication of newborns or children, guide clinical correct medication, avoid medication risks, reduce medication injury, and truly achieve precise medication.

The invention discloses a test strip RPA primer for detecting Ditylenchus destructor. The primer is composed of nucleotide sequences as shown in SEQ ID No:1 and SEQ ID No:2. The invention further discloses a combination of the primer and a probe, and the probe is composed of a sequence as shown in SEQ ID No:3. In addition, the invention discloses a detection kit comprising the primer and probe combination. The combination of the test strip RPA primer and the probe has the advantages of strong detection specificity for the Ditylenchus destructor, high sensitivity, low requirements on equipmentand personnel quality, simplicity in operation, high detection speed and low cost, and is suitable for port quarantine and basic level on-site detection.

The invention discloses a nucleic acid rapid detection kit for a geese origin component in food and feed and an application method of the kit, belonging to the fields of molecular biology and immunology. According to the invention, a high-sensitivity high-specificity method of PCR in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunological detection, extracted target DNA is subjected to specific amplification through designing a specific primer and labeling the primer and the amplified product is combined with a gold labeled antibody immobilized on the test strip in a developing liquid so as to form stable and visible detection zone and quality control zone, thereby realizing the rapid and accurate detection of geese origin component in food and feed.

The invention discloses a nucleic acid group suitable for detection of SNP sites of tumor drug-related genes and an application, and relates to the technical field of gene polymorphism detection. Thenucleic acid group comprises one or more of primer combination 1-primer combination 15. Polymorphism of 15 SNP sites of 9 tumor drug-related genes can be detected simultaneously by the nucleic acid group, and the nucleic acid group has the characteristics of low cost, simple result interpretation, no interference among SNP sites and the like.

The invention discloses a PIK3CA gene mutation fluorescence quantitative PCR genotype detection kit comprising a PCR mixed reaction liquid, a positive control, and a fluorescent probe used for detecting PIK3CA gene mutant genotypes. The PCR mixed reaction liquid comprises PCR primers used for amplifying PIK3CA gene segments where the mutation sites exist. The invention also discloses a PIK3CA gene mutation fluorescence quantitative PCR genotype detection method. According to the method, PIK3CA gene mutant genotypes are subjected to fluorescence quantitative PCR detections by using the kit provided by the invention. With the technical scheme provided by the invention, detections can be carried out upon tissue PIK3CA gene mutations, and especially rapid, accurate, and high-sensitively detections upon PIK3CA gene 542,545 and 1047 nucleotide mutations can be realized.

The invention discloses a preparation method and an application method of a nucleic-acid rapid detection kit for Gallusgallus ingredients in food and feeding stuff, and belongs to the fields of molecular biology and immunology. The detection kit is characterized in that the high-sensitivity and high-specificity method of the polymerase chain reaction in nucleic acid detection is combined with the immune colloidal gold rapid detection technology in immunological detection, unique primers are designed and marked, the extracted target DNA is specifically amplified and the product of the amplification is bonded with gold labeled antibodies immobilized on a test strip in a developing solution, and as a result, stable and visual detection zone and quality control zone are formed, and therefore, rapid and accurate detection on the Gallusgallus ingredients in the food and the feeding stuffing is realized.

The invention discloses a rapid nucleic acid test strip detection kit for a food-borne pathogenic microorganism cronobacter sakazakii and an application method of the kit, and belongs to the fields of molecular biology and immunology. According to the kit and the application method thereof, a high-sensitivity and high-specificity polymerase chain reaction method for nucleic acid detection is combined with an immune colloidal gold rapid-detection technology for immunological detection, unique primers are designed and labeled, extracted target DNA (deoxyribonucleic acid) is subjected to specific amplification, and an amplification product is bound with a gold labeled antibody fixed on a test strip in a developing solution to form a stable and visible detection strip and a stable and visible quality control strip, so that main food-borne pathogenic microorganisms are rapidly and accurately detected.

The present invention discloses a preparation method of a rapid nucleic acid detection kit for a donkey (Equusafricanusasinus) component in food and feed, and an application method thereof, and belongs to the field of molecular biology and immunology. According to the invention, the high sensitivity and high specificity polymerase chain reaction method in nucleic acid detection and the immune colloidal gold rapid detection technology in immunology detection are combined, the unique primers are designed, the primers are labeled, specific amplification is performed on the extracted target DNA, and the amplified product is bound with gold-labeled antibody immobilized on test paper strip in a spreading solution so as to form the stable and visible detection zone and the quality control zone, such that the rapid and accurate detection on the donkey source component in food and feed is achieved.

The invention discloses a reverse transcription loop-mediated isothermal amplification (RT-LAMP) kit for detecting pestedes petits ruminants viruses (PPRVs). The kit comprises an RT-LAMP primer, a 2X reaction buffer solution, an enzyme mixture (EM), a fluorescent visual detection reagent, ultrapure water and a reaction template, wherein the RT-LAMP primer includes outer primers F3 and B3, inner primers FIP and BIP and loop primers LF and LB. The detection kit is mainly used for detecting whether the PPRVs exist in lesion tissues suspiciously infected with the PPRVs. Specific detection and sensitivity detection prove that the RT-LAMP kit provided by the invention can monitor a reaction in real time and quantitatively detect a copy number of the PPRVs, a detection result is obtained quickly and accurately, and the convenience is brought for simply and quickly detecting the PPRVs.

The invention discloses an RET fusion gene ARMS (amplification refractory mutation system) fluorescent quantitative PCR typing detection kit and relates to a primer, a probe and a detection kit for detecting 7 common KIF5B-RET fusion gene variants and 1 RET fusion gene type ARMS-qPCR, wherein the RET fusion gene mutation comprises eight fusion variants including KR1(K15:R12), KR2(K16:R12), KR3(K23:R12), KR4(K24:R8), KR5(K22:R12), KR6(K24:R11), KR7(K15:R11) and newly discovered NR8(N6:R12). In the invention, the detection is fast, efficient and sensitive; the result interpretation is very clear and visual, and the result is reliable and peculiar; and by adopting the primer, probe and kit provided by the invention, 8 RET fusion gene mutations can be effectively detected.

This invention belongs to the moleculal biology field. It involves a kind of fluorescent quantitive PCR test kit of detection gene mutations. The test kit includes fluorescent quantitive PCR premixed liquid, fluorescent quantitive reaction liquid A, luorescent quantitive reaction liquid B (the feature is that it contains primer and anti-primer and fluorescence probes), positive comparison sample A, positive comparison sample B, positive comparison sample C. This invention features that: short detection time, easy operation, low quality requirement of sample DNA, the result reading is clear, direct with high sensitivity.

The present invention discloses a preparation method of a rapid nucleic acid detection kit for a duck (scientific name: Anas platyrhynchos) component in food and feed, and an application method thereof, and belongs to the field of molecular biology and immunology. According to the invention, the high sensitivity and high specificity polymerase chain reaction method in nucleic acid detection and the immune colloidal gold rapid detection technology in immunology detection are combined, the unique primers are designed, the primers are labeled, specific amplification is performed on the extracted target DNA, and the amplified product is bound with gold-labeled antibody immobilized on test paper strip in a spreading solution so as to form the stable and visible detection zone and the quality control zone, such that the rapid and accurate detection on the duck source component in food and feed is achieved.

The invention discloses primers and a reagent kit for detecting the polymorphism of the ALDH2 gene c.1510 locus. The specific primers, a specific probe and the specific reagent kit which are designed for the ALDH2 gene locus have the advantages of being high in sensitivity, good in specificity, high in response speed and low in cost, and are suitable for large-scale clinical application; rapid, effective and accurate typing qualitative detection of ALDH2 can be achieved, and a reference can be provided for timely nitroglycerin treatment of stenocardia, drinking guidance and occurrence of related caner.

The invention relates to an EML4-ALK (echinoderm microtubule associated protein like4-anaplastic lymphoma kinase) fusion gene fluorescent quantitative PCR (polymerase chain reaction) assay kit, which is applicable to assay of EML4-ALK fusion gene mutation in lung adenocarcinoma. The kit comprises probes, primers and positive controls, which are specially designed for conserved sequences of 9 fusion variations of the EML4-ALK fusion gene. The kit can be used for quickly and accurately assaying 9 most common EML4-ALK fusion gene variations with high sensitivity, namely 9 fusion variations of 7 variant subtypes, which are subtype 1 (E13; A20), subtype 2 (E20; A20), subtype 3 (E6a / b; A20), subtype 4 (E14; A20), subtype 5 (E2a / b; A20), subtype 6 (E18; A20) and subtype 7 (E14; A20), so that a real-time fluorescent quantitative PCR system for assaying 9 most common EML4-ALK fusion gene variations can be established.