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Primers and reagent kit for detecting polymorphism of ALDH2 gene c.1510 locus

A site polymorphism, ALDH2-FP technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve electrophoresis laboratory pollution, complex sequencing experiment operation, long experiment cycle, etc. problems, to achieve effective and accurate typing and qualitative detection, high specificity and sensitivity, and rapid response

Pending Publication Date: 2016-06-01
汪运山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sequencing experiment operation is more complicated, the experiment cycle is longer, and electrophoresis is easy to cause laboratory pollution.

Method used

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  • Primers and reagent kit for detecting polymorphism of ALDH2 gene c.1510 locus
  • Primers and reagent kit for detecting polymorphism of ALDH2 gene c.1510 locus
  • Primers and reagent kit for detecting polymorphism of ALDH2 gene c.1510 locus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] 1. Sample Processing

[0104] 1.1 Sample processing and nucleic acid extraction

[0105] This kit does not contain nucleic acid extraction components. Samples (No. 1, 2, and 3) are treated with commercial nucleic acid extraction kits, and TIANamp Blood DNA Kit (CatNO.DP318) is recommended. For specific operations, please refer to the kit manual, but pay attention to the following .

[0106] The concentration of DNA needs to be measured with a UV spectrophotometer, the sample has no protein or RNA contamination, OD 260 / OD 280 =1.8~2.0, OD 260 / OD 230 ≥2.0; DNA content requirements: The recommended DNA loading amount is 50-250ng; it is recommended to test the extracted DNA immediately, otherwise, please store it at -20±5°C.

[0107] 1.2 preparation

[0108] Before the start of the experiment, take out the components of the kit, fully thaw, shake continuously for 15 seconds to mix, and centrifuge at 2000rpm for 15 seconds.

[0109] Prepare a corresponding number of...

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PUM

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Abstract

The invention discloses primers and a reagent kit for detecting the polymorphism of the ALDH2 gene c.1510 locus. The specific primers, a specific probe and the specific reagent kit which are designed for the ALDH2 gene locus have the advantages of being high in sensitivity, good in specificity, high in response speed and low in cost, and are suitable for large-scale clinical application; rapid, effective and accurate typing qualitative detection of ALDH2 can be achieved, and a reference can be provided for timely nitroglycerin treatment of stenocardia, drinking guidance and occurrence of related caner.

Description

technical field [0001] The invention relates to a primer and a kit for detecting the c.1510 site polymorphism of ALDH2 gene. Background technique [0002] Angina pectoris is caused by coronary artery insufficiency caused by coronary atherosclerotic stenosis. Clinically, sublingual nitroglycerin is used for treatment. Nitroglycerin releases nitric oxide (NO). NO is the same as endothelial relaxation factor and activates the guanylate ring. Cytase increases the cyclic guanosine monophosphate (CGMP) in smooth muscle and other tissues, leads to the descaling of myosin light chain, regulates the contraction state of smooth muscle, causes vasodilation and relieves angina pectoris. Generally, sublingual administration of nitroglycerin takes effect within 15 minutes. If it cannot be relieved, it needs to be admitted to the hospital for treatment in time. Experiments have found that aldehyde dehydrogenase 2 (aldehydedehydrogenase2, ALDH2) is the key factor for the onset of nitroglyc...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q1/6858C12Q2600/156
Inventor 王军韩淑毅张慧林朱鹏冲徐祎慧王敏张孝乾刘沛周婷刘妍妍
Owner 汪运山
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