Cultivating method of hyriopsis cumingii with golden yellow shell
A technology of spinnaker mussels and cultivation methods, applied in fish farming, application, climate change adaptation, etc., can solve the problems of not paying attention to the breeding of mussels, environmental pollution, large economic losses, etc. The effect of high post-survival rate and stable shell color genetics
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Embodiment 1
[0024] Embodiment one: a kind of cultivation method of golden-yellow shell color Triangle sail clam, comprises the following steps:
[0025] (1) On March 10, 2010, 581 parent clams of the 4th to 6th instar with strong water spout, soft belly edge and complete shell were selected from the wild population of Poyang Lake. The male and female were identified by observing the gill filament spacer. 323 female mussels and 258 male mussels were selected, and the shell surfaces of the selected male and female broodstock were marked;
[0026] (2) Take 50 mg of the mantle edge tissue of male and female mussels, and use the column type animal tissue total RNA extraction and purification kit (Shanghai Sangon Bioengineering Co., Ltd.) to extract total RNA; use the AMV first-strand cDNA synthesis kit to synthesize single Strand cDNA; SRAP amplification was carried out with a specific primer combination, and the amplified products were subjected to agarose electrophoresis and observed under u...
Embodiment 2
[0032] Embodiment two: a kind of cultivation method of golden-yellow shell color triangular sail clam, comprises the following steps:
[0033] (1) On March 20, 2011, 483 parent clams of the 4th to 6th instar with strong water spout, soft belly edge and complete shell were selected from the wild population of Poyang Lake. The male and female were identified by observing the gill filament spacer. 272 female mussels and 211 male mussels were obtained, and the shell surfaces of the selected male and female broodstock were marked;
[0034] (2) Take 50 mg of the mantle edge tissue of male and female mussels, and use the column type animal tissue total RNA extraction and purification kit (Shanghai Sangon Bioengineering Co., Ltd.) to extract total RNA; use the AMV first-strand cDNA synthesis kit to synthesize single Strand cDNA; SRAP amplification was carried out with a specific primer combination, and the amplified products were subjected to agarose electrophoresis and observed under...
Embodiment 3
[0040] Embodiment three: a kind of cultivation method of golden-yellow shell color Triangle sail clam, comprises the following steps:
[0041] (1) On March 20, 2012, 1,710 parent mussels of the 4th to 6th instar with strong water spout, soft belly edge and complete shell were selected from the wild population of Poyang Lake, and male and female were identified by observing the gill filament spacer. 1124 female mussels and 586 male mussels were produced, and the shell surfaces of the selected male and female broodstock were marked;
[0042] (2) Take 50 mg of the mantle edge tissue of male and female mussels, and use the column type animal tissue total RNA extraction and purification kit (Shanghai Sangon Bioengineering Co., Ltd.) to extract total RNA; use the AMV first-strand cDNA synthesis kit to synthesize single Strand cDNA; SRAP amplification was carried out with a specific primer combination, and the amplified products were subjected to agarose electrophoresis and observed ...
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