43 results about "Sequence-related amplified polymorphism" patented technology

The invention discloses a method for identifying peach bud mutation. The method comprises the following steps: firstly, carrying out continuous three-generation grafting identification, observing the stability of phenotypic character, collecting tender leaves and parental tender leaves of suspected bud mutation with stable characters after the continuous three-generation grafting, extracting genome DNA, carrying out PCR (Polymerase Chain Reaction) amplification on the DNA by using an SSR (Signal Sequence Repeat) primer and an SRAP (Sequence Related Amplified Polymorphism) primer respectively, carrying out bud mutation distinguishing on amplification products through agarose gel electrophoresis, and if electrophoresis bands of parent and the suspected bud-mutant breed are different, identifying the suspected bud-mutant breed as bud mutation. By means of the method disclosed by the invention, 'small white peach' and early-maturing bud-mutant 'Jinliuzaohong' peach are successfully identified. The method disclosed by the invention is simple and convenient in operation, wide in application and convenient to popularize, and saves a lot of human and material resources.

The invention discloses a bacterial gene resource screening and storing method which includes the steps: (1) separating strains; (2) extracting DNA (deoxyribonucleic acid) of the strains; (3) building a matrix table; (4) acquiring a screening scheme; (5) storing representative bacterial strains. An SRAP (sequence-related amplified polymorphism) molecular marker method based on bacterial genome information adopts specific primers and is stable and high in purposefulness.

The invention relates to a cultivating method of hyriopsis cumingii with a golden yellow shell. The method is characterized by comprising the following steps of (1), selecting 4-6 parent hyriopsis cumingii; (2) taking the margin tissues of the pallium of the parent hyriopsis cumingii, extracting the total RNA (ribonucleic acid) and synthetic single stranded c RNA, performing SRAP (sequence-related amplified polymorphism) amplification by adopting primer combination, and selecting male and female individuals with specific strip sequences with the golden yellow shells after the amplification products are subjected to electrophoresis; (3) using the selected male and female parent hyriopsis cumingii to build a family and cultivating in a water flowing pool; (4) periodically examining the pregnant condition of the female hyriopsis cumingii, and if mature glochidiums are found, selecting the mature female hyriopsis cumingii to reproduce to obtain infant hyriopsis cumingii; cultivating the infant hyriopsis cumingii in the water flowing pool until the shell reaches 1-2cm long, removing into to a net cage, and suspending the net cage into a pond to perform second stage cultivating on the infant hyriopsis cumingii until the shell length reaches 5-8cm; (5) removing the family parent hyriopsis cumingii with the non-golden yellow shells, and using the rest parent hyriopsis cumingii to build a core cultivating population with the golden yellow shells. According to the cultivation method of the hyriopsis cumingii with the golden yellow shell, the ratio of the hyriopsis cumingii with the golden yellow shell can reach more than 99.0%, the shell color is stable in heredity and the postoperative survival rate is high.

The invention relates to a method for molecularly identifying the genetic markers and the variety of a plant, and specifically discloses a group of SRAP (Sequence-Related Amplified Polymorphism) marking primers and an identification method for identifying Suzhi No.1 hybrid zoysia. The group of primers is used for PCR (Polymerase Chain Reaction) to obtain the amplified fragments of each pair of primers; the obtained fragments are compared with genome fragments disclosed by the invention; consequently, whether the zoysia variety is the Suzhi No.1 hybrid zoysia can be identified accurately.

The invention belongs to the fields of molecular biology DNA (deoxyribonucleic acid) marking technology and applications, and relates to a molecular marker for porphyra yezoensis high-temperature-resistant strain TM-18 and a construction method for the same. Specifically, a porphyra yezoensis strain obtained by artificial selection and breeding is analysed by virtue of an SRAP (sequence-related amplified polymorphism) marking technology, and the specific bands of the high-temperature-resistant strain TM-18 are found via PCR (polymerase chain reaction) electrophoresis detection; and specific primers are designed via recovering and sequencing, and converted to SCAR (sequence characterized amplified regions) markers for extension and strain verification. A method for identifying porphyra yezoensis high-temperature-resistant strain TM-18 by the molecular marker comprises the following steps of: extracting the total DNA of porphyra yezoensis; performing PCR amplification by the specific primers; performing electrophoresis detection on amplification products; and performing result judgement, specifically, if amplification products with a length of 341 bp are generated, then judging that the strain is a high-temperature-resistant strain TM-18. In the method, identification can be performed only by one-time simple PCR and agarose electrophoresis; and the method is simple and rapid to operate, and free from the influence of environmental factors, and is an ideal means of strain identification.