The invention relates to fish feed, in particular to mixed feed for juvenile fishes of oreochromis sp. The invention further relates to a preparing method for the mixed feed for the juvenile fishes of the oreochromis sp. The mixed feed for the juvenile fishes of the oreochromis sp comprises, by mass, 18-29 parts of fish meal, 5-15 parts of soybean straw, 6-12 parts of salvinia natans powder, 5-12 parts of dry green grass powder, 3-8 parts of rhizoma phragmitis, 2-7 parts of common dayflower herbs, 2-6 parts of oysters, 3-7 parts of poria cocos, 2-5 parts of platycladi seeds, 2-6 parts of rhizoma corydalis, 1-5 parts of fructus alpiniae oxyphyllae, 1-4 parts of angelica sinensis, 2-6 parts of fish oil, 1-4 parts of honey, 1-4 parts of olive oil and 1-4 parts of gypsum. After the juvenile fishes of the oreochromis sp are fed on the mixed feed, the survival rate of the juvenile fishes of the oreochromis sp is high, the feed coefficient is low, the specific growth rate is high, and the disease resisting ability is high.