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35 results about "Oreochromis" patented technology

Oreochromis is a large genus of oreochromine cichlids, fishes endemic to Africa and the Middle East. A few species from this genus have been introduced far outside their native range and are important in aquaculture. Many others have very small ranges; some are seriously threatened, and O. ismailiaensis and O. lidole possibly are extinct. Although Oreochromis primarily are freshwater fish of rivers, lakes and similar habitats, several species can also thrive in brackish waters and some even survive in hypersaline conditions with a salinity that far surpasses that of seawater. In addition to overfishing and habitat loss, some of the more localized species are threatened by the introduction of other, more widespread Oreochromis species into their ranges. This is because they—in addition to compete for the resources—often are able to hybridize.

Method for improving overwintering survival rate and qualities of oreochromis niloticus fries

The invention discloses a method for improving overwintering survival rate and qualities of oreochromis niloticus fries. Before overwintering, feeds with 12% to 14% fat level are fed to the fries, and soybean oil and fish oil are blent in a proportion of 4:1 to form fat sources. Fatty acids--linoleic acids and linolenic acids which are necessary to growth of oreochromis niloticus are mainly increased so as to improve energy reserve. Low-temperature stress resistance of the oreochromis niloticus can be improved by low temperature acclimatization and the low temperature adaptability is improved. During overwintering, feeds with 1.5% to 2% compound Chinese herbal medicine are fed to the fries, so that damages of the low-temperature stress to fish bodies can be effectively relieved, the non-specificity immunity of the fish bodies is improved, and the overwintering survival rate and fry qualities are improved. Meanwhile, according to the above three steps, material selection and facility configuration are easy, the price is relatively low, processes are simple, traditional problems that overwintering costs are high, the survival rate of the fries is low, and qualities of the fries are poor are excellently solved, and environment-friendly and physically beneficial breeding requirements can be met.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

SNP marker relevant with growth speed of Oreochromis niloticus and application of SNP marker

The invention discloses an SNP marker relevant with the growth speed of Oreochromis niloticus and application of the SNP marker. The SNP marker is a basic group G or T at a site 501 from 5' terminal of a nucleotide sequence represented by SEQ ID NO: 1. The SNP marker is closely relevant with the growth speed of the Oreochromis niloticus and can be effectively applied to molecular marker assistant breeding of the Oreochromis niloticus.
Owner:海南华大海洋科技有限公司

Method for improving streptococcus iniae infection-resisting capacity of GIFT oreochromis niloticus during high-temperature stress

The invention discloses a method for improving the streptococcus iniae infection-resisting capacity of GIFT oreochromis niloticus during high-temperature stress. The method comprises the following steps: preparing basic ration and a compound Chinese medicinal herb formula, and feeding feed. Due to the adoption of the method, the immunity index of the GIFT oreochromis niloticus during high-temperature stress can be effectively increased, the liver damage is reduced, meanwhile, the streptococcus iniae infection-resisting capacity of the GIFT oreochromis niloticus can be improved, the morbidity is reduced, and the survival rate of the GIFT oreochromis niloticus infected with streptococcus iniae can be increased. Several Chinese medicinal herbs involved in the method are relatively available and relatively low in cost; a production technology is simple. The basic ration with the compound Chinese medicinal herbs has relatively good palatability, and after the basic ration is taken by the GIFT oreochromis niloticus, drug residues in bodies of the GIFT oreochromis niloticus are relatively few, and therefore, the GIFT oreochromis niloticus meets the requirements on green healthy aquatic products.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

Real-time fluorescence PCR (polymerase chain reaction) method and primer pair for identifying gender of oreochromis niloticus

The invention relates to a real-time fluorescence PCR (polymerase chain reaction) method for identifying the gender of oreochromis niloticus, and belongs to the technical field of molecular biology. The research comprises selecting gender-related gene AMH of oreochromis niloticus to design a pair of specific real-time fluorescence PCR primers, wherein the expression quantities of the gene in male fish and female fish have significant difference, the gender of the oreochromis niloticus can be identified by comparing the expression quantity of gene AMH with the expression quantity of adult fish ovary AMH gene. The method provided by the invention is strong in specificity, high in reliability and can be used for identifying the gender of tilapia mossambica at fingerling stage.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

Oreochromis niloticus sex chromosome specific molecular marker

The invention belongs to the technical field of genetic engineering and particularly relates to an oreochromis niloticus sex chromosome specific molecular marker, namely an X chromosome specific molecular marker NtX1, which has a nucleotide sequence represented by SEQ ID No.3; through polymerase chain reaction(PCR) amplification, the X chromosome specific molecular marker NtX1 and a Y chromosome specific molecular marker NtY1 and / or a Y chromosome specific molecular marker NtY2 can be used to differentiate YY supermale oreochromis niloticus, XY male oreochromis niloticus and XX female oreochromis niloticus and promote the mass production of all-male oreochromis niloticus fry through a YY supermale oreochromis niloticus breeding route; therefore, the breeding output of oreochromis niloticus is increased obviously, the breeding cost is reduced, and the economic benefit is increased.
Owner:SOUTHWEST UNIVERSITY

Preparation method of feed capable of protecting liver of GIFT oreochromis niloticus and boosting immunity of GIFT oreochromis niloticus and feeding method of feed

The invention discloses a preparation method of feed capable of protecting the liver of GIFT oreochromis niloticus and boosting the immunity of the GIFT oreochromis niloticus and a feeding method of the feed. The preparation method comprises the steps of feed preparation and chitosan addition. The feeding method of feed comprises the step of feed feeding. After the GIFT oreochromis niloticus is fed for 9 weeks by adopting the feeding method, the immunity index of the GIFT oreochromis niloticus is obviously increased, the liver stress is decreased, the oxidative stress resistance capacity of the liver is enhanced, the activity of superoxide dismutase, catalase, glutathione and glutathion peroxidase in the liver is obviously improved, and the level of the malonaldehyde is lowered; the level of glucose, cholesterol and low-density cholesterol in serum is obviously lowered; the level of alkaline phosphatase and high-density cholesterol is obviously increased. The chitosan added to the feed is metabolites generated by fermentation of microorganisms without antagonism and is small in molecular weight, easy to absorb, low in cost and relatively easily available. After the feed is taken by the GIFT oreochromis niloticus, the feed is easy to digest and absorb by the GIFT oreochromis niloticus, and residues are barely remained in bodies; the feed has a very good immunopotentiator effect and meets the requirements on novel green healthy additives.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI +2

Compound feed capable of improving reproductive capacity of Oreochromis niloticus parent fish and preparation method thereof

The invention discloses a compound feed capable of improving the reproductive capacity of Oreochromis niloticus parent fish and a preparation method thereof. The compound feed can obviously improve the reproductive capacity of Oreochromis niloticus parent fish and reinforce the disease resistance. The feed comprises the following ingredients in percentage by weight: fish meal 5-15%, soybean meal 10-30%, dephenolized cottonseed meal 5-15%, double-low rapeseed meal 10-20%, high-gluten flour 10-30%, corn protein powder 5-10%, rice bran 10-20%, phosphatide oil 0.5-1%, soybean oil 1-3%, compound vitamins 0.2-0.5%, compound minerals 1.0-2.0%, phagostimulant 0.3-1.0%, vitamin C phosphate 0.1-0.3%, calcium dihydrogen phosphate 1-3%, anti-mildew agent 0.01-0.05%, Temianhuang 0.2-0.8%, choline chloride 0.05-0.15%, and vitamin E 0.01-0.03%. The feed and the preparation method thereof belong to the technical field of aquatic feeds.
Owner:TONGWEI

Method for reducing fat deposition in livers of Oreochromis niloticus during high-density cultivation

The invention discloses a method for reducing fat deposition in the livers of Oreochromis niloticus during high-density cultivation. The method comprises the following steps: preparation of a basic ration; preparation of a compound Chinese herbal medicine; addition of the compound Chinese herbal medicine; and feeding of fries. Compared with the prior art, the invention has the following advantages: (1) the method provided by the invention can effectively improve the absorption and utilization of fat in feeds, reduce fat deposition in livers, lower the incidence rate of fatty livers and promote aquaculture of healthy Oreochromis niloticus; (2) the compound Chinese herbal medicine used in the method is a green and environment-friendly preparation, is small in residues in Oreochromis niloticus and low in negative effect on environment, and conforms to requirements on green and environmental-friendly feed additives; and (3) with a compound Chinese herbal medicine feed used in the invention, the absorption efficiency of Oreochromis niloticus is good, no residue is left in the body of Oreochromis niloticus, and excrement of the Oreochromis niloticus is free of pollution to environment.
Owner:江苏恒泰环保科技发展有限公司

Method for increasing survival rate of adults of live oreochromis niloticus

InactiveCN103749360ARegulate energy reservesRegulatory responsivenessClimate change adaptationAnimal feeding stuffAnti stressHouttuynia
The invention discloses a method for increasing the survival rate of adults of live oreochromis niloticus. The hormone level, the alexin level and the hepatic antioxidant adaptive capacity can be improved within a short time by increasing breeding density and performing tensioning net stress, and nonspecific immunity is improved. The contents of protein, glucose and triglyceride in blood of the oreochromis niloticus can be increased by feeding feedstuff with high protein level of 36%-39% before the oreochromis niloticus is conveyed, and energy reserve is improved. Moreover, 0.5%-0.8% of compound heartleaf houttuynia herb is added in the feedstuff and contains herba houttuyniae, scutellaria baicalensis, radix isatidis, fructus forsythiae, honeysuckle and the like, and the anti-stress capability of the oreochromis niloticus can be improved. In a conveying process, the water temperature is controlled in a range of 19-23 DEG C, the salinity of water is 8%o-12%o, and dissolved oxygen is sufficient. The metabolic activity of the oreochromis niloticus is low, but the immunity of the oreochromis niloticus is high, so that energy loss in the conveying process is reduced, excretion of metabolin is reduced, and the anti-stress capability is improved. After the oreochromis niloticus is treated by using the method, the survival rate of the adults of the live oreochromis niloticus and the live oreochromis niloticus conveying distance are obviously improved during conveyance.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

Recombinant lactococcus lactis and vaccine for Streptococcus agalactiae disease of Oreochromis sp.

The invention discloses recombinant lactococcus lactis based on the fbsA gene of Streptococcus agalactiae. The recombinant lactococcus lactis comprises the fbsA gene of Streptococcus agalactiae or segments of the fbsA gene, the fbsA gene or the segments of the fbsA gene preferably has (or have) a sequence shown in SEQ ID No. 1, the deposit number of the recombinant lactococcus lactis is CCTCC M 2020140, and expression of recombinant FbsA protein with a theoretical molecular weight of 34.6 kDa can be induced under the culture condition that niusin exists. The invention also provides a vaccine for the Streptococcus agalactiae disease of Oreochromis sp., and the vaccine is prepared from the recombinant lactococcus lactis, and is capable of producing protective antibodies in Oreochromis sp. after Oreochromis sp. is fed with the vaccine in an immune mode of oral gavage, so that Oreochromis sp. is assisted to resist infection of Streptococcus agalactiae effectively.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI

Method for breeding GIFT, oreochromis niloticus

The invention discloses a method for breeding GIFT, oreochromis niloticus. The breeding method includes the steps that fodder is fed to the GIFT, oreochromis niloticus, the throwing amount of the fodder is 1.5%-3% the body weight of the oreochromis niloticus, and the throwing frequency of the fodder is twice a day; according to the breeding conditions, the water temperature is 30-40 DEG C, the pH value is 7.2-7.8, the dissolved oxygen content is 6-7 mg / L, and the ammonia nitrogen content is smaller than 0.1 mg / L. By the adoption of the breeding method, the fodder throwing amount of the GIFT, oreochromis niloticus can be reduced, meanwhile, the quality of the fed GIFT, oreochromis niloticus is good, and the physiological property is good.
Owner:GUANGXI ACADEMY OF FISHERY SCI

Pond ecological poly-culture method of oreochromis niloticus and macrobrachium rosenbergii

InactiveCN107197791AImplement GradientsStaggered time to marketSpecific water treatment objectivesClimate change adaptationShrimpWater quality
The invention discloses a pond ecological poly-culture method of oreochromis niloticus and macrobrachium rosenbergii. The pond ecological poly-culture method comprises the following steps: step 1, culture pond construction: a plurality of net cages are arranged in a culture pond, have a certain distance from the edge of the culture pond and are not in contact with the bottom of the culture pond; step 2, fry and young shrimp putting: firstly, macrobrachium rosenbergii young shrimps are put in a space outside the net cages in the culture pond, and then, oreochromis niloticus fries are put in the net cages; step 3, feeding management: firstly, macrobrachium rosenbergii feed is fed on the periphery of the culture pond, and then, oreochromis niloticus feed is fed into the net cages. The pond ecological poly-culture method discloses by the invention has the benefits that the farmer breeding cost is reduced, the pond water quality is regulated, and the yield of the oreochromis niloticus and the macrobrachium rosenbergii can also be improved.
Owner:GUANGXI ACADEMY OF FISHERY SCI

Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales

The invention discloses a method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales. The method comprises the steps as follows: the oreochromis niloticus skins and scales are cleaned and drained, soaked in hydrochloric acid of 0.4-0.6 mol / L for 20-60 min for decalcification and soaked in sodium hydroxide of 0.1-0.6 mol / L for 30-60 min for degreasing; then the skins and scales and water in a solid-to-liquid ratio of (8-12):100 are added into a fermentation tank to be sterilized at 121 DEG C for 30 min; strains are inoculated into the fermentation tank, and fermentation is conducted at 35 DEG C for 24-48 h; fungus bodies and impurities are removed by filtration after fermentation is finished, and a filtrate I is collected; and the filtrate I is subjected to ultrafiltration through an ultrafiltration membrane so as to obtain a filtrate II containing the protein peptide and a trapped fluid containing the plasmin. According to the method, two products, namely, the protein peptide with the antihypertensive activity and the plasmin capable of dissolving thrombus, are produced simultaneously with the fermentation method directly, so that the raw material utilization rate is high, the production cost is significantly reduced, and the method is environment-friendly and free from environmental pollution and has the wide application value.
Owner:SHENZHEN UNIV

Mixed feed for juvenile fishes of oreochromis sp and preparing method thereof

The invention relates to fish feed, in particular to mixed feed for juvenile fishes of oreochromis sp. The invention further relates to a preparing method for the mixed feed for the juvenile fishes of the oreochromis sp. The mixed feed for the juvenile fishes of the oreochromis sp comprises, by mass, 18-29 parts of fish meal, 5-15 parts of soybean straw, 6-12 parts of salvinia natans powder, 5-12 parts of dry green grass powder, 3-8 parts of rhizoma phragmitis, 2-7 parts of common dayflower herbs, 2-6 parts of oysters, 3-7 parts of poria cocos, 2-5 parts of platycladi seeds, 2-6 parts of rhizoma corydalis, 1-5 parts of fructus alpiniae oxyphyllae, 1-4 parts of angelica sinensis, 2-6 parts of fish oil, 1-4 parts of honey, 1-4 parts of olive oil and 1-4 parts of gypsum. After the juvenile fishes of the oreochromis sp are fed on the mixed feed, the survival rate of the juvenile fishes of the oreochromis sp is high, the feed coefficient is low, the specific growth rate is high, and the disease resisting ability is high.
Owner:QINGDAO HAIZHIYUAN INTELLIGENT TECH

Oreochromis niloticus alexin C9 gene clone and application thereof

Oreochromis niloticus alexin C9 gene clone and application thereof. The invention belongs to functional study of the Oreochromis niloticus infected Streptococcus agalactiae. The invention discloses immune-related gene OnC9 of Oreochromis niloticus, and the nucleotide sequence is shown in the table SEQ ID NO.1. And the invention discloses the sequence of the immune-related protein OnC9 of Oreochromis niloticus, and the amino acid sequence thereof is shown in the sequence table SEQ ID NO.2. The method uses the disclosed nucleotide sequence of Oreochromis niloticus OnC9 to design a primer of fluorogenic quantitative PCR detection (C9-qf:5'-GGCTTGAAGTCGCTCTCCA-3'; C9-qr:5'-GCCAAATGCTTCTACACTTCG-3'), and carries out the fluorogenic quantitative PCR detection for the healthy Oreochromis niloticus or Oreochromis niloticus infected Streptococcus agalactiae. The method can be used for researching immune response of Oreochromis niloticus after infecting Streptococcus agalactiae.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI

Prevention and treatment method and treatment method of common diseases of oreochromis mossambila

The invention discloses a prevention and treatment method of common diseases of oreochromis mossambila. The prevention and treatment method comprises the steps that a pond is built; healthy fry is selected, and disinfection is carried out; pond disinfection is carried out; the throw-in density of the fry is controlled; a chemical additive is added to feed; pond prevention and treatment are carriedout. The pond is built, and the probability of the diseases of fish bodies is reduced from the source; the health fry are selected, disinfection is carried out, miscellaneous fishes, various spawns,aquatic insects, parasites and pathogenic bacteria can be fast killed in the pond, the reasonable throw-in density is specified, freshness of water and the environmental sanitation of the pond are kept, the diseases are eliminated in time, the high-quality feed is adopted, vitamin B, C and E is added, and different agents are added corresponding to different seasons; meanwhile, a four-fixed mode is used for feeding, the vitality of mycetozoa is reduced, and the diseases are prevented from being generated.
Owner:富宁县沣民农业发展有限公司

Oreochromis sp formula feed and preparation method thereof

The invention relates to a fish feeder, in particular to an oreochromis sp formula feed, and a preparation method of the oreochromis sp formula feed. The oreochromis sp formula feed comprises the following components in parts by mass: 22 to 31 parts of fish meal, 7 to 15 parts of corn protein powder, 7 to 14 parts of stillage, 4 to 9 parts of bone powder, 6 to 12 parts of herba hyperici japonica, 3 to 7 parts of barbed skullcap herb, 3 to 7 parts of nutgrass galingale rhizome, 2 to 7 parts of pagoda tree flower bud, 2 to 6 parts of Indian kalimeris herb, 1 to 5 parts of platycodon grandiflorum, 1 to 4 parts of broom cypress, 3 to 8 parts of fiddlehead, 2 to 6 parts of fish oil, 1 to 4 parts of honey, 1 to 4 parts of olive oil, and 1 to 4 parts of monocalcium phosphate. The oreochromis sp formula feed has the advantages that after feeding, the coefficient of the oreochromis sp is low; in muscles of the oreochromis sp, the content of crude protein is high, the content of crude fat is low, and the content of flavor amino acid is high.
Owner:QINGDAO HAIZHIYUAN INTELLIGENT TECH

Process for obtaining decellularized extracellular matrix, decellularized extracellular matrix, use thereof and kit

The present invention describes a process for obtaining extracellular matrix from the skin of tilapia (Oreochromis niloticus) comprising the steps of chemical and enzymatic decellularization, detoxification, chemical disinfection, crosslinking, bleaching, dehydration and sterilization by gamma radiation, more specifically the steps comprised by each of said procedures and use of the extracellular matrix for treating ruptured of tissues, dermatitises, acute, chronic and traumatic lesions, battlefield wounds, necrotic wounds, lacerations, abrasions, bruises and other lesions and conditions. The present invention falls within the fields of pharmacy, medicine and veterinary medicine, dentistry, chemistry, tissue engineering, molecular biology and biotechnology.
Owner:JUNIOR EDMAR MACIEL LIMA +1

A feed preparation method and feeding method for liver protection and immunity enhancement of Gifu tilapia

The invention discloses a preparation method of feed capable of protecting the liver of GIFT oreochromis niloticus and boosting the immunity of the GIFT oreochromis niloticus and a feeding method of the feed. The preparation method comprises the steps of feed preparation and chitosan addition. The feeding method of feed comprises the step of feed feeding. After the GIFT oreochromis niloticus is fed for 9 weeks by adopting the feeding method, the immunity index of the GIFT oreochromis niloticus is obviously increased, the liver stress is decreased, the oxidative stress resistance capacity of the liver is enhanced, the activity of superoxide dismutase, catalase, glutathione and glutathion peroxidase in the liver is obviously improved, and the level of the malonaldehyde is lowered; the level of glucose, cholesterol and low-density cholesterol in serum is obviously lowered; the level of alkaline phosphatase and high-density cholesterol is obviously increased. The chitosan added to the feed is metabolites generated by fermentation of microorganisms without antagonism and is small in molecular weight, easy to absorb, low in cost and relatively easily available. After the feed is taken by the GIFT oreochromis niloticus, the feed is easy to digest and absorb by the GIFT oreochromis niloticus, and residues are barely remained in bodies; the feed has a very good immunopotentiator effect and meets the requirements on novel green healthy additives.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI +2

A method to reduce hepatic fat deposition in high-density culture of Gifu tilapia

The invention discloses a method for reducing fat deposition in the livers of Oreochromis niloticus during high-density cultivation. The method comprises the following steps: preparation of a basic ration; preparation of a compound Chinese herbal medicine; addition of the compound Chinese herbal medicine; and feeding of fries. Compared with the prior art, the invention has the following advantages: (1) the method provided by the invention can effectively improve the absorption and utilization of fat in feeds, reduce fat deposition in livers, lower the incidence rate of fatty livers and promote aquaculture of healthy Oreochromis niloticus; (2) the compound Chinese herbal medicine used in the method is a green and environment-friendly preparation, is small in residues in Oreochromis niloticus and low in negative effect on environment, and conforms to requirements on green and environmental-friendly feed additives; and (3) with a compound Chinese herbal medicine feed used in the invention, the absorption efficiency of Oreochromis niloticus is good, no residue is left in the body of Oreochromis niloticus, and excrement of the Oreochromis niloticus is free of pollution to environment.
Owner:江苏恒泰环保科技发展有限公司

Real-time fluorescence PCR (polymerase chain reaction) method and primer pair for identifying gender of oreochromis niloticus

The invention relates to a real-time fluorescence PCR (polymerase chain reaction) method for identifying the gender of oreochromis niloticus, and belongs to the technical field of molecular biology. The research comprises selecting gender-related gene AMH of oreochromis niloticus to design a pair of specific real-time fluorescence PCR primers, wherein the expression quantities of the gene in male fish and female fish have significant difference, the gender of the oreochromis niloticus can be identified by comparing the expression quantity of gene AMH with the expression quantity of adult fish ovary AMH gene. The method provided by the invention is strong in specificity, high in reliability and can be used for identifying the gender of tilapia mossambica at fingerling stage.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

Tilapia (oreochromis niloticus) myosin light chain 3 promoter

ActiveUS20160113252A1Enhance protein expressionHigh expressionVectorsSugar derivativesSkeletal muscleOreochromis
The preset invention relates to a promoter to target a fluorescent protein to the muscles of fish, such as A. nigrofasciatus, for ornamental purposes, which is a Mlc3 (myosin, light polypeptide 3, skeletal muscle) promoter. The Mlc3 promoter has the nucleotides of tilapia (Oreochromis niloticus) myosin light chain 3 (Mlc3) promoter region, which is potential to be a tilapia Mlc3 promoter to enhance protein expression in muscle of fish, particularly for the generation of ornamental fish.
Owner:ACAD SINIC +1

Vaccination of Sex Reversed Hybrid Tilapia (Oreochromis niloticus X O. aureus) With an Inactivated Vibrio vulnificus Vaccine

Vibrio vulnificus can cause infections in aquaculture-raised fish and is considered an opportunistic human pathogen. We isolated V. vulnificus from diseased hybrid tilapia (Oreochromis niloticus X O. aureus) cultured in a North American water reuse aquaculture facility. We have characterized the isolate using biochemical and molecular methods, developed a disease infection model, and determined that formalin-inactivated whole-cell vaccine provides protection against V. vulnificus. The V. vulnificus isolate was determined to be biotype 1, 16S rRNA type B, vcg type C, and vvhA type 2. Fish vaccinated with the formalin-inactivated whole-cell vaccine responded to vaccination as measured by agglutinating antibody titer. In two separate trials, vaccinated tilapia exhibited relative percent survival of 73 and 60% following challenge with the homologous isolate. In additional trials, vaccinated tilapia exhibited survival values of up to 87.5% following challenge with a heterologous isolate. Use of a mineral oil adjuvant enhanced protection.
Owner:UNITED STATES OF AMERICA

A method to improve the ability of Gifu tilapia to resist Streptococcus iniae infection under high temperature stress

InactiveCN103734537BImprove immune indicatorsImprove infection abilityAnimal feeding stuffMedicinal herbsBiology
The invention discloses a method for improving the streptococcus iniae infection-resisting capacity of GIFT oreochromis niloticus during high-temperature stress. The method comprises the following steps: preparing basic ration and a compound Chinese medicinal herb formula, and feeding feed. Due to the adoption of the method, the immunity index of the GIFT oreochromis niloticus during high-temperature stress can be effectively increased, the liver damage is reduced, meanwhile, the streptococcus iniae infection-resisting capacity of the GIFT oreochromis niloticus can be improved, the morbidity is reduced, and the survival rate of the GIFT oreochromis niloticus infected with streptococcus iniae can be increased. Several Chinese medicinal herbs involved in the method are relatively available and relatively low in cost; a production technology is simple. The basic ration with the compound Chinese medicinal herbs has relatively good palatability, and after the basic ration is taken by the GIFT oreochromis niloticus, drug residues in bodies of the GIFT oreochromis niloticus are relatively few, and therefore, the GIFT oreochromis niloticus meets the requirements on green healthy aquatic products.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

A kind of method for improving the survival rate of live transport of adult tilapia fish

InactiveCN103749360BImprove the survival rate of long-distance transportationReduce excretionClimate change adaptationAnimal feeding stuffAnti stressHouttuynia
The invention discloses a method for increasing the survival rate of adults of live oreochromis niloticus. The hormone level, the alexin level and the hepatic antioxidant adaptive capacity can be improved within a short time by increasing breeding density and performing tensioning net stress, and nonspecific immunity is improved. The contents of protein, glucose and triglyceride in blood of the oreochromis niloticus can be increased by feeding feedstuff with high protein level of 36%-39% before the oreochromis niloticus is conveyed, and energy reserve is improved. Moreover, 0.5%-0.8% of compound heartleaf houttuynia herb is added in the feedstuff and contains herba houttuyniae, scutellaria baicalensis, radix isatidis, fructus forsythiae, honeysuckle and the like, and the anti-stress capability of the oreochromis niloticus can be improved. In a conveying process, the water temperature is controlled in a range of 19-23 DEG C, the salinity of water is 8%o-12%o, and dissolved oxygen is sufficient. The metabolic activity of the oreochromis niloticus is low, but the immunity of the oreochromis niloticus is high, so that energy loss in the conveying process is reduced, excretion of metabolin is reduced, and the anti-stress capability is improved. After the oreochromis niloticus is treated by using the method, the survival rate of the adults of the live oreochromis niloticus and the live oreochromis niloticus conveying distance are obviously improved during conveyance.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

Batch breeding method for redhump eartheaters

The invention discloses a batch breeding method for redhump eartheaters. A soil-based pond with muddy at the bottom is used as a spawning pond, a flat-bottomed ceramic container that is put upside down at the bottom of the pond is used as an egg attaching device, parent fishes are selected and freely bred in a season with a suitable temperature according to a proper male-to-female ratio, fertilized ova are hatched in a shaded aquarium, and fries are cultivated in a fish tank. The batch breeding method has the advantages of high spawning rate, high survival rate in hatching, few fry loss in hatching, high fry yield, simple operation flow and easiness in implementation, and can be applicable to large-scale fry production of oreochromis niloticus and fishes laying eggs on stone plates.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI

Method for improving overwintering survival rate and qualities of oreochromis niloticus fries

InactiveCN103734060BIncrease resistance to low temperature stressImprove non-specific immunityClimate change adaptationAnimal feeding stuffFish oilOreochromis
The invention discloses a method for improving overwintering survival rate and qualities of oreochromis niloticus fries. Before overwintering, feeds with 12% to 14% fat level are fed to the fries, and soybean oil and fish oil are blent in a proportion of 4:1 to form fat sources. Fatty acids--linoleic acids and linolenic acids which are necessary to growth of oreochromis niloticus are mainly increased so as to improve energy reserve. Low-temperature stress resistance of the oreochromis niloticus can be improved by low temperature acclimatization and the low temperature adaptability is improved. During overwintering, feeds with 1.5% to 2% compound Chinese herbal medicine are fed to the fries, so that damages of the low-temperature stress to fish bodies can be effectively relieved, the non-specificity immunity of the fish bodies is improved, and the overwintering survival rate and fry qualities are improved. Meanwhile, according to the above three steps, material selection and facility configuration are easy, the price is relatively low, processes are simple, traditional problems that overwintering costs are high, the survival rate of the fries is low, and qualities of the fries are poor are excellently solved, and environment-friendly and physically beneficial breeding requirements can be met.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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