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SNP marker relevant with growth speed of Oreochromis niloticus and application of SNP marker

A technology of gift tilapia and growth rate, which can be used in the determination/inspection of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problem of molecular markers to be excavated, etc., and achieve low-cost effects

Active Publication Date: 2017-09-29
海南华大海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, molecular markers related to growth traits that can be effectively used in tilapia breeding at this stage still need to be discovered

Method used

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  • SNP marker relevant with growth speed of Oreochromis niloticus and application of SNP marker
  • SNP marker relevant with growth speed of Oreochromis niloticus and application of SNP marker
  • SNP marker relevant with growth speed of Oreochromis niloticus and application of SNP marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Acquisition of SNP markers related to the growth rate of Gifu tilapia

[0032] 1.1 Acquisition of Gifu tilapia populations

[0033] The group used was the Gifu tilapia hatched on April 10, 2016 in the farm of Hainan Huada Ocean Technology Co., Ltd. On April 20, 2016, 20,000 fry were transferred to a cage for further breeding. On October 8, 2016, 500 individuals were randomly selected from the cage, and the dorsal fin rays of the fish were cut and stored in 95% ethanol at -20°C for genomic DNA extraction.

[0034] 1.2 Genomic DNA extraction of Gifu tilapia

[0035] In this experiment, the conventional phenol-chloroform method was used to extract the genomic DNA in the fin rays of Gifu tilapia, and the specific steps were as follows:

[0036] (1) Take 0.3-0.5g of fin rays in a 1.5ml Eppendorf tube, cut it into pieces, and dry it for 20 minutes on a clean bench;

[0037] (2) After the ethanol is basically volatilized, wash with TE buffer solution (10mmol / ml Tr...

Embodiment 2

[0042] Example 2 Sequencing verification and application of SNP markers related to the growth rate of Gifu tilapia

[0043] 2.1 Extraction of genomic DNA from fin rays of Gifu tilapia to be tested

[0044] The Gifu tilapia to be tested came from the Gifu tilapia population in Example 1, and 200 fish were randomly selected again, and the genomic DNA was extracted according to the DNA extraction method described in Example 1.

[0045] 2.2 Amplification of nucleotide fragments containing SNP sites

[0046] Using the genomic DNA of each tested Gifu tilapia obtained from the aforementioned extraction as a template, use the forward primer F: 5'-CATTCATATGGAGTTGTGCATCT-3' (SEQ ID NO: 2) and the reverse primer R: 5'-GAAAGAGGCAAACCTCACACCTT-3 '(SEQ ID NO: 3), the nucleotide fragment where the SNP to be detected is located is amplified. Among them, the PCR reaction system is calculated as 25 μl: 50-100ng / μl template DNA 1μl, 10pmol / μl primer F and R 1μl each, 10mmol / LdNTP mix 2.0μl, 5...

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Abstract

The invention discloses an SNP marker relevant with the growth speed of Oreochromis niloticus and application of the SNP marker. The SNP marker is a basic group G or T at a site 501 from 5' terminal of a nucleotide sequence represented by SEQ ID NO: 1. The SNP marker is closely relevant with the growth speed of the Oreochromis niloticus and can be effectively applied to molecular marker assistant breeding of the Oreochromis niloticus.

Description

technical field [0001] The present invention relates to SNP markers and their applications. Specifically, the present invention relates to SNP markers related to the growth rate of Gifu tilapia, primer pairs and kits for detecting the aforementioned SNP markers, the use of the aforementioned SNP markers, primer pairs, and kits in the breeding of Gifu tilapia, And a method for detecting the growth rate of Gifu tilapia. Background technique [0002] Tilapia belongs to Perciformes, Cichlidae, and Tilapia. It is a euryhalal tropical fish and has been listed as one of the six major human foods by the United Nations Food and Agriculture Organization (FAO). At present, tilapia farming has spread to more than 80 countries and regions around the world, becoming a worldwide farmed fish. Since the 1990s, my country's tilapia industry has maintained a good momentum of sustainable development. In 2016, the global tilapia production was 5.55 million tons, of which China's tilapia produc...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 游欣欣林学强易云海
Owner 海南华大海洋科技有限公司
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