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Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales

The technology of tilapia and protein peptide is applied in the biological field, which can solve the problems of expensive addition and high production cost, and achieve the effects of low cost and simple production process.

Inactive Publication Date: 2014-05-28
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of collagen by enzymatic hydrolysis requires the addition of expensive proteases, resulting in high production costs
At present, there is no method for simultaneously producing protein peptides and plasmin by using tilapia skin scales as raw materials and using microbial fermentation

Method used

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  • Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales
  • Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales
  • Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 5kg of tilapia skin, wash and drain, add 40L of 0.2mol / L sodium hydroxide, soak for 30min to degrease, wash with water until neutral, crush and dry for use. Add fish skin and water to the fermenter at a solid-liquid mass ratio of 8:100, sterilize at 121°C for 30 minutes. Bacillus subtilis was inoculated with 2% of the inoculum, and the fermentation temperature was 35°C for 24 hours. At the end of the fermentation, the fermentation broth is filtered by plate frame to remove bacteria and impurities, and the filtrate is collected. The filtrate was ultrafiltered with a 5000 dalton ultrafiltration membrane, the filtrate was protein peptide, and the retentate was plasmin. The filtrate was concentrated in vacuo and spray-dried to obtain protein peptide powder. Plasmin was freeze-dried to obtain active enzyme powder.

Embodiment 2

[0031] Weigh 5kg of the mixture of tilapia skin and fish scales, wash and drain, add 60L of hydrochloric acid solution with a concentration of 0.4mol / L, soak for 30min to decalcify, wash with water until neutral and drain; then add 0.4mol / L sodium hydroxide 60 liters, soaked for 40 minutes to degrease, washed with water until neutral, crushed and dried for later use. Add the mixture of fish skin and fish scales and water to the fermentation tank at a ratio of solid-liquid mass ratio of 12:100, sterilize at 121° C. for 30 minutes. Bacillus subtilis was inoculated at an inoculum of 4%, and the fermentation temperature was 35°C for 36 hours. At the end of the fermentation, the fermentation broth is filtered by plate frame to remove bacteria and impurities, and the filtrate is collected. The filtrate was ultrafiltered with a 5000 dalton ultrafiltration membrane, the filtrate was protein peptide, and the retentate was plasmin. The filtrate was concentrated in vacuo and spray-drie...

Embodiment 3

[0033] Weigh 5kg of the mixture of tilapia skin and fish scales, wash and drain, add 60L of hydrochloric acid solution with a concentration of 0.6mol / L, soak for 30min to decalcify, wash with water until neutral and drain; add 80L of 0.6mol / L sodium hydroxide, Degreased for 60 minutes, washed with water until neutral, crushed and dried for later use. Add fish skin and water to the fermenter at a ratio of 10:100 solid to liquid, sterilize at 121°C for 30 minutes. 6% of the inoculum was inoculated with Bacillus subtilis, and the fermentation temperature was 35°C for 48 hours. At the end of the fermentation, the fermentation broth is filtered by plate frame to remove bacteria and impurities, and the filtrate is collected. The filtrate was ultrafiltered with a 5000 dalton ultrafiltration membrane, the filtrate was protein peptide, and the retentate was plasmin. The filtrate was concentrated in vacuo and spray-dried to obtain protein peptide powder. Plasmin was freeze-dried to o...

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Abstract

The invention discloses a method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales. The method comprises the steps as follows: the oreochromis niloticus skins and scales are cleaned and drained, soaked in hydrochloric acid of 0.4-0.6 mol / L for 20-60 min for decalcification and soaked in sodium hydroxide of 0.1-0.6 mol / L for 30-60 min for degreasing; then the skins and scales and water in a solid-to-liquid ratio of (8-12):100 are added into a fermentation tank to be sterilized at 121 DEG C for 30 min; strains are inoculated into the fermentation tank, and fermentation is conducted at 35 DEG C for 24-48 h; fungus bodies and impurities are removed by filtration after fermentation is finished, and a filtrate I is collected; and the filtrate I is subjected to ultrafiltration through an ultrafiltration membrane so as to obtain a filtrate II containing the protein peptide and a trapped fluid containing the plasmin. According to the method, two products, namely, the protein peptide with the antihypertensive activity and the plasmin capable of dissolving thrombus, are produced simultaneously with the fermentation method directly, so that the raw material utilization rate is high, the production cost is significantly reduced, and the method is environment-friendly and free from environmental pollution and has the wide application value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing protein peptides and plasmin by fermentation of tilapia skin and fish scales. Background technique [0002] Plasmin is a proteolytic enzyme that can specifically degrade fibrin gel and is an important component of the fibrinolytic system. Plasmin mainly degrades fibrin into small soluble fragments by degrading fibrin and fibrinogen, hydrolyzing various coagulation factors, converting plasminogen into plasmin, hydrolyzing complement, etc. Cleared from the circulatory system. Plasmin acts on fibrinogen and fibrin, promotes the release of tissue plasminogen activator from endothelial cells, enhances its activity, and has antithrombotic function; it can reduce platelet aggregation and blood viscosity, reduce myocardial oxygen consumption, Improve microcirculation. [0003] Protein peptides are molecular polymers between amino acids and proteins, which can be as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12N9/68A23L1/29A61K8/64A61K38/01A61K38/48A61P7/02C12R1/125A23L33/00
Inventor 韩庆国魏文丰
Owner SHENZHEN UNIV
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