Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker for Fenneropenaeus chinensis with high-pH resistance character and identification method using same

A Chinese prawn, molecular marker technology, applied in DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of long selection cycle, less research and application, and more time-consuming, and achieve the effect of short screening cycle and simple and convenient operation.

Inactive Publication Date: 2011-08-17
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technique has been widely used in plant genetic diversity analysis (Budak H, 2004), hybrid vigor estimation (Riaz A, 2001), comparative genomics research (Li G, 2003), genetic linkage map construction (Lin Z X, 2009) and related gene mapping (Alwala, 2009), but there are few research applications in the field of aquatic animal research, only in Macrobrachium rosenbergii (Macrobrachium Rosenbergii ) (Zhou Jinsong, 2006), Macrobrachium japonica ( Macrobrachium nipponensis ) (Yao Jianhua, 2008) and grass carp ( Ctenopharyngodon idellus ) (Zhang Zhiwei, 2007; Ding Weidong, 2008) have seen reports
[0005] Traditional breeding methods mainly use phenotypic differences to select good traits, but due to the influence of environmental factors and the limitation of selection intensity, it is difficult to select traits with low heritability, and the selection cycle is long and time-consuming. Molecular breeding methods have emerged as the times require, and The rapid development of molecular marker technology provides technical support
At present, the selection of traits in Chinese shrimp breeding includes growth rate (Liu Ping, 2007; He Yuying, 2007) and disease resistance (Meng Xianhong, 2005; Yue Zhiqin, 2005), and the combination of selection and environmental adverse factors, molecular markers Assisted breeding has not been reported at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker for Fenneropenaeus chinensis with high-pH resistance character and identification method using same
  • Molecular marker for Fenneropenaeus chinensis with high-pH resistance character and identification method using same
  • Molecular marker for Fenneropenaeus chinensis with high-pH resistance character and identification method using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The technical scheme of the present invention includes two steps: (1) Screening and cloning of specific SRAP markers related to the high pH tolerance trait of Chinese prawns, and converting them into specific SCAR markers; (2) Establishing an identification method for the high pH tolerance traits of Chinese prawns.

[0037] (1) Screening and cloning of specific SRAP markers related to high pH tolerance of Penaeus Chinese shrimp, and converting them into specific SCAR markers includes the following steps:

[0038] 1. High pH stress test of Chinese prawns and extraction of muscle DNA

[0039] 1) Chinese shrimp high pH stress test

[0040] Random sampling of 50 healthy Chinese prawns from each family in the experimental base was temporarily kept in the corresponding enclosure of the indoor cement pool, and the daily management was strict and unified. After three days of temporary breeding, use NaOH to adjust the pH value of the water body to 9.2 (semi-lethal pH value) for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a molecular marker for Fenneropenaeus chinensis with a high-pH resistance character and an identification method using the same. The polymerase chain reaction (PCR) identification method for the high-pH resistance character of Fenneropenaeus chinensis is established based on a specific sequence responsible for the high-pH resistance character of Fenneropenaeus chinensis and designed specific primers, and the specific sequence responsible for the high-pH resistance character of Fenneropenaeus chinensis is obtained by SRAP (sequence-related amplified polymorphism) marker screening, cloning, sequencing, specific primer design and specific marker verification. The method for screening the specific SRAP marker to the high-pH resistance character of Fenneropenaeus chinensis is established to select one primer pair composition from 110 SRAP primer pair compositions to produce a high-pH resistance specific SRAP fragment. The PCR identification method for the high-pH resistance character of Fenneropenaeus chinensis is established for the first time. The identification method is advanced, stable and reliable; and has significance and wide prospects in application for stress resistance breeding of Fenneropenaeus chinensis, enhancement of environmental adaptability of Fenneropenaeus chinensis, and large-area farming of Fenneropenaeus chinensis.

Description

technical field [0001] The invention belongs to the field of genetic engineering of aquatic biotechnology, and relates to molecular markers and genetic identification technology related to shrimp tolerance to high pH, ​​in particular to a molecular marker and identification method of Chinese prawn tolerance to high pH. Background technique [0002] Chinese prawns ( Fenneropenaeus chinensis ) Natural resources are mainly distributed in my country's Yellow Sea, Bohai Sea and the coast of the Korean Peninsula. In the early 1990s, the output reached more than 200,000 tons, ranking first in the world for many years. Among them, the production of prawn farming in China accounted for more than 70% of the total production of prawn farming in the country. Landslides, the annual output has been hovering around 50,000 tons for a long time, and the resulting economic losses have reached tens of billions of yuan. During the breeding process, the growth rate slowed down and the ability ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/11C12Q1/68
Inventor 陈华增李健王清印何玉英李吉涛陈萍
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products