Dendrobium DNA (Deoxyribonucleic Acid) fingerprint spectrum and special primer and establishment method for dendrobium DNA fingerprint spectrum
A technology of DNA fingerprinting and fingerprinting, which is applied in the field of plant DNA fingerprinting to achieve the effect of facilitating computer identification and analysis
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Embodiment 1
[0029] In this example, 20 widely representative Dendrobium strains collected were used as test materials, DNA was extracted from tissue cultured seedlings, SRAP analysis was performed, and corresponding fingerprints were established. The obtained fingerprints were used to identify these 20 Dendrobium species, and provided an effective method for reference for germplasm identification of other Dendrobium species. The concrete operation of setting up the dendrobium DNA fingerprint is as follows:
[0030] 1. Selection of representative Dendrobium species
[0031] The present embodiment adopts 20 parts of Dendrobium species shown in Table 2, including 1 part of Dendrobium nobile Lindl., 1 part of Dendrobium huoshanense, and 18 parts of Dendrobium officinale. The materials are selected to be widely planted in production. Dendrobium strains, see Table 1. In this example, DNA was extracted from 20 dendrobium leaves, and the DNA fingerprints of the 20 dendrobium strains were establ...
Embodiment 2
[0053] Dendrobium dendrobium DNA fingerprint of the present invention is used for germplasm identification of dendrobium
[0054] Assuming that SH in the test material is an excellent variety used in production, it cannot be determined whether the existing batch of Dendrobium is the real Dendrobium SH-15. Its DNA fingerprint can be identified as follows.
[0055] After the dendrobium standard DNA fingerprint is established, when a sample is to be authenticated, extract the DNA of the sample to be tested, use the extracted DNA as a template, use ME1 and EM7; ME1 and EM14; ME3 and EM3; ME3 and EM5; The 6 pairs of primers ME8 and EM14; ME9 and EM3 were amplified by SRAP-PCR, and the amplified products were electrophoresed on a 4% denatured polyacrylamide gel, and silver-stained to draw the DNA fingerprint of the sample to be tested. By comparing the standard DNA fingerprints, it is possible to know whether the variety is the real Dendrobium SH-15.
[0056] The results showed th...
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