Method for identifying peach bud mutation

An identification method and budding technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of incomplete sequences, etc., and achieve the effects of wide application, easy promotion, and saving manpower and material resources

Inactive Publication Date: 2014-05-28
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
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Problems solved by technology

Studies have found that the number of repeat units in microsatellites is highly variable, and these variations are manifested as euploid variations in the number of microsatellites or sequences in repeat unit sequences that may not be completely identical, resulting in polymorphism at multiple sites

Method used

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  • Method for identifying peach bud mutation
  • Method for identifying peach bud mutation
  • Method for identifying peach bud mutation

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Embodiment Construction

[0039] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0040] Material:

[0041] The present invention selects the early-maturing peach variety 'Prunus persica cv. Xiaobaitao' and its new bud strain 'Jinliuzaohong (Prunus persica cv.Jinliuzaohong)', both from the peach breeding nursery of Tianjin Xuexiang Fruit and Vegetable Co., Ltd. .

[0042] 1. Identification of grafting

[0043] ⑴ In the orchard of Tianjin Xuexiang Fruit and Vegetable Co., Ltd. in late May 2004, a 3-year-old common peach tree (commonly known as white peach in the local area) was found. The two fruits on the back of a main branch were particularly large and had begun to grow. color. At that time, the whole tree, including other trees of the same species in the entire orchard, prod...

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Abstract

The invention discloses a method for identifying peach bud mutation. The method comprises the following steps: firstly, carrying out continuous three-generation grafting identification, observing the stability of phenotypic character, collecting tender leaves and parental tender leaves of suspected bud mutation with stable characters after the continuous three-generation grafting, extracting genome DNA, carrying out PCR (Polymerase Chain Reaction) amplification on the DNA by using an SSR (Signal Sequence Repeat) primer and an SRAP (Sequence Related Amplified Polymorphism) primer respectively, carrying out bud mutation distinguishing on amplification products through agarose gel electrophoresis, and if electrophoresis bands of parent and the suspected bud-mutant breed are different, identifying the suspected bud-mutant breed as bud mutation. By means of the method disclosed by the invention, 'small white peach' and early-maturing bud-mutant 'Jinliuzaohong' peach are successfully identified. The method disclosed by the invention is simple and convenient in operation, wide in application and convenient to popularize, and saves a lot of human and material resources.

Description

Technical field: [0001] The invention belongs to the field of horticultural crop breeding, and relates to a method for breeding new varieties of fruit trees, in particular to a method for identifying peach bud mutations. Background technique: [0002] Budding selection of fruit trees has the characteristics of short breeding cycle and fast breeding process, and is an important way to innovate germplasm resources. Budding is a kind of somatic mutation, which is a mutation that occurs in the somatic cells of the meristem of plant buds. Budding is generally manifested in the characteristics and characteristics of branches, leaves, flowers, fruits, phenological stages, and mature stages. Select mutant buds and their growing branches, and through asexual reproduction, new varieties can be created, called bud mutation selection. For example, many varieties of marshal apples are obtained by using bud mutation selection. [0003] Peach is one of the fruit trees that are prone to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 冯涛梁发辉卢兴霞崔雁黎于玮玮李慧李爱
Owner TIANJIN AGRICULTURE COLLEGE
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