74 results about "Porphyra yezoensis" patented technology

The invention belongs to molecular biology DNA labeling technique and application field, concretely relates to a porphyra yezoensis microsatellite marker screening method, and a method for analyzing germplasm genetic diversity by the makers, thereby lay foundations for genetic structure analysis of porphyra yezoensis, germplasm resource protection and molecular marker auxiliary breeding.

The invention discloses a method and device for automatically washing picked porphyra yezoensis shoots. The device for automatically washing the picked porphyra yezoensis shoots comprises a submerged pump and a water inlet pipe. The water inlet pipe is shaped like a Chinese character 'hui'. One end of the water inlet pipe is connected with the submerged pump and the other end of the water inlet pipe is provided with three arm type rotary nozzles. The interval of the nozzles arranged on the same line ranges from 1.5 m to 4 m. Two rows of parallel nozzles are arranged in a staggered mode. The spraying range of the nozzles is a circle with the radius ranging from 1 m to 2 m. Each nozzle is composed of a hollow shaft core and three arm type water outlet pipes. The hollow shaft cores are connected with the water inlet pipe. Water outlets are evenly formed in the arm type water outlet pipes. After stimulation of the day and night temperature difference and the running water stimulation, conchospores are diffused by shell protonemata in a concentrated mode in the morning of a next day, high-density conchospore water in a nursery pond is pumped to the water inlet pipe through the submerged pump and sprayed to a shoot picking net curtain through the three arm type rotary nozzles, and therefore a whole nursery system is washed synchronously and evenly. The device is simple in structure and operation, quite low in constructive cost, low in use cost and better in shoot picking effect.

Disclosed is a method for removing green algae in the middle and late culture stages of Porphyra yezoensis. The method is characterized by comprising (1) preparing 50-100ml of 10% sodium hypochlorite solution, adding cleaned and disinfected seawater to reach a volume of 1L, adding trisodium phosphate and disodium phosphate at molar ratio of 3:1 to the above solution, and adjusting pH to 9.0-9.5 for later use; (2) during the culture process after nori mesh screens are placed into the sea (i.e. from September 21 to April of the next year), performing smear operation in the sea; (3) during the process to clear green algae, adding analytical grade citric acid to the solution of step (1) to allow pH value of the solution to be about 5.0-5.5; (4) smearing the solution obtained in step (3) in batches every day to green algae attached to mesh screens, raft frames and cables so that the damaged green algae break under the effect of seawater flow and enter the sea; and (5) smearing with the solution obtained in step (3) once every two months. The method of the invention can remove green algae attached to mesh screens, raft frames and cables in the middle and late culture stages of Porphyra yezoensis.

The invention relates to a stereoscopic culturing system and method of porphyra yezoensis shell protonema. The stereoscopic culturing system comprises a plurality of culturing tanks which are distributed stereoscopically, a water storage tank is arranged under the culturing tanks and leads water into the culturing tanks through a water circulation system, and the culturing tanks are each further provided with an artificial light source used for culturing shell protonema. The culturing method includes the steps that light is regulated, wherein 400-500 nm of blue light and 600-700 nm of red light are the main, and light sources at other spectral lines are also taken into account; the intensity ratio of the red light to the blue right is regulated between 4:1 and 12:1; light intensity is controlled between 500 Lux and 5000 Lux; the daily illumination time of the artificial light sources is regulated between 8 hours and 18 hours. The stereoscopic culturing system and method of porphyra yezoensis shell protonema can improve the reliability of seedling culturing production, shorten the culturing period of shell protonema, flexibly arrange the harvesting time of conchospore, and largely reduce the production cost.

The invention discloses a method for measuring the ascorbic acid content in porphyra yezoensis by high performance liquid chromatography. In the method, the reverse high performance liquid chromatography is adopted; the chromatographic conditions of the reverse high performance liquid chromatography are that: a chromatographic column is a hydrophilic column (AQ) taking octadecyl silane as a filler; the chromatographic column can well retain substances with relatively high polarities, and take pure water as a mobile phase; a chromatographic mobile phase is 0.01 mol / L monopotassium phosphate (pH2.5)-methanol(95: 5, V / V); a detector is an ultraviolet detector with the detection wavelength of 265 nanometers; the flow speed is 1 ml / min; the sample inlet volume is 20 mu L; and the temperature of the column is 25 DEG C. According to the method, the ascorbic acid content in the porphyra yezoensis can be measured accurately and conveniently; and the method has the characteristics of high sensitivity and high reproducibility, and is easy to operate.

The invention relates to primary-taste nori and a making technology thereof, and belongs to the technical field of nori. The primary-taste nori disclosed by the invention comprises the following raw materials in parts by mass: 1-5 parts of dried porphyra yezoensis and 0.1-0.5 part of composite seasoning fluid, wherein the composite seasoning fluid comprises the following raw materials in parts by mass: 20-24 parts of a soy sauce, 18-21 parts of white granulated sugar, 17-20 parts of glucose syrup, 11-13 parts of high fructose corn syrup, 4-6 parts of monosodium glutamate, 3-5 parts of edible salt, 1-3 parts of a yeast extract, 0.04-0.06 part of disodium 5'-ribonucleotide and 0.001-0.003 part of edible essence. The making technology of the primary-taste nori comprises the following steps of performing screening, supplying dried porphyra yezoensis, performing baking for the first time, feeding fluid for seasoning, performing baking for the second time and performing baking for the third time. The primary-taste nori disclosed by the invention is rich in nutrition, pure in taste, natural in material usage, hygienic and safe.

The invention discloses a method for preparing a porphyra anti-oxidation peptide and comprehensively utilizing byproducts, and belongs to the technical field of natural active substance extraction. According to the method, porphyra yezoensis is used as a raw material and subjected to pretreatment including drying, crushing and the like, the porphyra raw material is subjected to enzymolysis by using neutral proteinase and aminopeptidase under specific conditions to obtain a coarse product of the anti-oxidation peptide, the coarse product is separated and purified through membrane technologies of ultra-filtration, nano-filtration and the like to obtain the porphyra anti-oxidation peptide, and the byproducts such as porphyra polysaccharides and porphyra dietary fibers in the enzymolysis process are reclaimed and extracted. The molecular weight of the porphyra anti-oxidation peptide extracted by adopting the method is mainly distributed below 1000Da, the optimal acting pH is 8.5, and the heat stability is good; the yield of the porphyra anti-oxidation peptide is 73.1%, the recovery rate of the porphyra polysaccharides is 55%, and the recovery rate of the dietary fibers is 85%. The porphyra anti-oxidation peptide extracted by adopting the method is small in molecular weight, high in oxidation resistance and good in stability, and has a good application prospect in the fields of food preservatives, cosmetic additives and the like.

The invention provides an green and environment-friendly hand-washing-free disinfectant gel and a preparing method thereof, and relates to the field of sanitation supplies. The environment-friendly hand-washing-free disinfectant gel comprises, by weight, 2-4% of macromolecular gel matrix, 50-80% of lower alcohol, 0.1-0.8% of extractive of banlangen, 0.1-0.8% of artemisia annua extract, 0.1-0.8% of licorice extract, 0.5-1% of aiye leaf extract, 0.5-1% of clove oil, 0.1-0.8% of porphyra yezoensis crude polysaccharide extract, 0.1-1% of aloe extracted liquid, 0.1-0.5% of honeysuckle extract, 0.1-0.5% of kariyt extract, 0.2-0.8% of seaweed livin, 0.05-0.1% of lecithin, 1-5% of wheat-germ oil, 0.5-1% of Tween 80, 0.01-0.15% of a pH controlling agent and the balance water. The environment-friendly hand-washing-free disinfectant gel uses the lower alcohol and a plant extracted liquid as synergistic antiseptic anti-inflammatory ingredients, skin caring and moisturizing ingredients are added, the prepared hand-washing-free disinfectant gel is stable in performance and has the effects of quick sterilization, green environmental protection, no irritant and both-hand skin caring, hand feeling is fresh and cool after the environment-friendly hand-washing-free disinfectant gel is used, quick drying is achieved, and drying and chapping of hand skin can be prevented effectively.

The invention discloses a method for separating an alpha-glucosidase inhibitor from a laver enzymolysis product, which belongs to the technical field of separation and purification of biological products. The invention relates to a method for compounding porphyra yezoensis to obtain a rough alpha-glucosidase inhibitor product, wherein the enzymolysis is controllable and the cost is low. The rough product is separated and purified through SPSepharoseHighPerformance cation exchange chromatography, SephadexG-10 gel chromatography and Mono Q anion exchange chromatography, and the purity and main composition of an LGI (lactate glucose index) part with high inhibitory activity on alpha-glucosidase are analyzed through reversed phase high performance liquid chromatography and ultra-high performance liquid chromatography-mass spectrography. The IC 50 value of LGI on the alpha-glucosidase is 97.63mu g / ml, and the temperature and pH stability are good; the inhibitory activity is kept to be higher than 80% in 80 DEG C water bath for 7 h, and the inhibitory activity is still kept to be higher than 87% after being maintained in a buffer solution with pH of 3-12 at 50 DEG C for 2 h. The LGI is small in molecular weight, good in stability, low in cost and safe to use, thereby having a quite wide application prospect in such fields as medicine, food, etc.

The invention discloses a porphyra yezoensis seedling collecting automatic even flushing method. A water pipe is arranged above a nursery pond, a flushing connecting pipe is arranged on the water pipe, the water pipe transversely moves along a transverse rail and longitudinally moves along a longitudinal rail, and therefore a net curtain in the nursery pond can be evenly flushed. According to the porphyra yezoensis seedling collecting automatic even flushing method and device, operation is easy to conduct, the device is simple and small in investment, labor cost is saved, flushing is more even, and the seedling collecting effect is better.

The NR enzymes described herein were discovered in the red algae of Porphyra perforata (PpNR) and Porphyra yezoensis (PyNR). The present invention provides methods and compositions relating to altering NR activity, nitrogen utilization and / or uptake in plants. The invention relates to a method for the production of plants with maintained or increased yield under low nitrogen fertility. The invention provides isolated nitrate reductase (NR) nucleic acids and their encoded proteins. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Plants transformed with nucleotide sequences encoding the NR enzyme show improved properties, for example, increased yield and growth.

The invention provides a method for preventing and controlling green alga overgrowth on porphyra cultivation raft frames and relates to the application of coatings such as water tank paint on the prevention and control over green alga overgrowth on the porphyra cultivation raft frames. The method comprises the steps that green alga overgrowth prevention paint is smeared on the porphyra cultivation raft frames, and the green alga overgrowth prevention paint is finishing paint, shipbottom paint, the water tank paint and the like. The method for preventing and controlling the green alga overgrowth on the porphyra cultivation raft frames can be used for preventing and controlling the green alga overgrowth on the cultivation raft frames in the cultivation industries of porphyra yezoensis and porphyra haitanensis.

The invention relates to the technical field of vegetarian meat food, in particular to a seafood vegetarian meat sauce and a preparation method thereof. Sugar, soy sauce, salt, monosodium glutamate seasoning, adding chitosan antioxidant, through a series of processes to make seafood vegetarian meat sauce with both nutrition and delicious effects; the beneficial effects of the invention are: health care, seafood taste; rich nutrition, strips Seaweed contains high iodine content, which can be used to treat "goiter" caused by iodine deficiency. Seaweed has the function of softening and resolving hard masses; it has dual functions of supplementing nutrition and health care; adding pure natural antioxidants makes eating more at ease; not only can It maintains a taste no less than that of fresh products, and has a long shelf life.

The invention provides a method for preserving porphyra yezoensis. The method comprises the following steps: (1) carrying out low-temperature vacuum drying on newly-harvested porphyra yezoensis at the temperature of 50 DEG C and the pressure of -0.05MPa, so as to form dried porphyra; (2) carrying out low-temperature cold storage on the dried porphyra at a temperature below 0 DEG C. The method has the following technical effects that the porphyra yezoensis can be fully dried, and protein, gelatin, various vitamins, such as vitamin A, vitamin B1, vitamin B2, vitamin C and the like, and other extracted ingredients and pigments contained by the porphyra can not be lost, so that the flavor and nutritive value of the porphyra are kept.

The invention discloses a PyTPS (porphyra yezoensis ueda trehalose-6-phosphate synthase) gene which is cloned to construct a plant expression vector of the gene, and the plant expression vector is transferred into the agrobactrium tumefaciens strain, so that an engineering strain is obtained. A rice variety is transformed by utilizing agrobacterium-mediated transformation, so that genetically modified rice is obtained; after growing and acclimatization, a T0 individual plant is obtained, and a T1 seed is collected; a seedling germinated from the T1-generation genetically modified seed receives PCR (polymerase chain reaction) detection and a salt tolerance assay, so that a T1-generation genetically modified salt-resistant strain is obtained, a T2 generation receives a kanamycin resistance assay, PCR detection and Southern hybridization, and results indicate that the PyTPS gene is already integrated into the genome of the genetically modified strain. The PyTPS gene in the invention has a TPS domain as well as a TPP (thiamine pyrophosphate) domain; the PyTPS gene source is safe; the development of the plant morphology of the PyTPS genetically modified rice is normal; the PyTPS gene can be stably inherited in genetically modified plants; and moreover, the transformation system is highly effective.

The invention provides a method for preventing and treating yellow leaf blotch of shell conchocelis of porphyra yezoensis and a culture apparatus. The method comprises the following steps: step S1, regulating pH value of soaking liquid; step S2, soaking the shell conchocelis in the soaking liquid obtained in the step S1; and step S3, setting interval time, and repeating soaking operation in the step S2. The culture device comprises a culture box, wherein a culture unit is arranged on the top of the culture box; the culture unit is divided into three culture media along a box body in the transverse direction; and the compositions and the structures of the culture media are the same. The method has the beneficial effects that the time of treatment on the shell conchocelis of the porphyra yezoensis can be shortened by acid treatment, the efficiency is improved, and meanwhile, costs can be reduced; and by the culture apparatus, the step of liquid drainage after culture can be simplified, meanwhile, culture time is monitored automatically, and the circumstance that the accuracy of a test result is affected due to deviation of time factor in a culture process is avoided.

The invention discloses a porphyra yezoensis mutation breeding method based on pigment mutant selection. The porphyra yezoensis mutation breeding method comprises the steps of: carrying out induced mutation, carrying out enzymolysis and separation on differentiated cells, selecting good regenerated thallus according to an improved variety selection target for carrying out single-strain culture, qualitatively and quantitatively determining the selected good pigment mutant regenerated thallus; carrying out enzymolysis, culturing into regenerated thallus to obtain diploid pure-line protonema through a parthenogenesis technology, and culturing into F1-generation thallus; and crushing the good pure-line protonema and then carrying out a shell seedling culturing test, picking seedlings from shell protonema on a culture web curtain, and carrying out a thallus pigment mutant good strain culturing test in a sea area. According to the invention, the breeding of the large cultivation algae porphyra yezoensis is switched into early-stage prediction and judgment in a laboratory from the later-stage sea cultivation test detection, stable inheritance of the porphyra yezoensis pigment mutant is obtained by using ray radiation treatment, and mutants relevant to the breeding target trait are selected to be used as breeding targets for carrying out breeding.

The invention discloses a construction method of porphyra yezoensis plastid genetic transformation vector, which comprises the steps of building skeleton vector containing rrsB-trnI-trnA-rrsL homologous recombination segments, building porphyra yezoensis plastid expression vector containing antibiotic screening gene expression cassettes, and building porphyra yezoensis plastid genome targeted integration expressed vector. The method adopts the thought of plastid genetic transformation, and provides a vector basis for plastid genetic transformation, so as to realize the advantages that the expression efficiency of plastid genetic transformation foreign gene is high, gene from pronucleus is not required to be modified, the safety is good, clon is easy to keep, and posterity is not separated.

The invention belongs to the fields of molecular biology DNA (deoxyribonucleic acid) marking technology and applications, and relates to a molecular marker for porphyra yezoensis high-temperature-resistant strain TM-18 and a construction method for the same. Specifically, a porphyra yezoensis strain obtained by artificial selection and breeding is analysed by virtue of an SRAP (sequence-related amplified polymorphism) marking technology, and the specific bands of the high-temperature-resistant strain TM-18 are found via PCR (polymerase chain reaction) electrophoresis detection; and specific primers are designed via recovering and sequencing, and converted to SCAR (sequence characterized amplified regions) markers for extension and strain verification. A method for identifying porphyra yezoensis high-temperature-resistant strain TM-18 by the molecular marker comprises the following steps of: extracting the total DNA of porphyra yezoensis; performing PCR amplification by the specific primers; performing electrophoresis detection on amplification products; and performing result judgement, specifically, if amplification products with a length of 341 bp are generated, then judging that the strain is a high-temperature-resistant strain TM-18. In the method, identification can be performed only by one-time simple PCR and agarose electrophoresis; and the method is simple and rapid to operate, and free from the influence of environmental factors, and is an ideal means of strain identification.

Disclosed is a method for detecting effects of Porphyra yezoensis polysaccharide PY-D2 on tumor resistance and immunity enhancement. The method for detecting effects of Porphyra yezoensis polysaccharide PY-D2 on tumor resistance and immunity enhancement is mainly characterized in that the detection method adopts the following steps of: polysaccharide extraction and purification; detecting the tumor cell growth by MTT assay; analyzing the cell cycle of tumor cells with a flow cytometer; data processing; preparation and primary culture of mouse spleen lymphocyte; detecting the survival rate of mouse spleen lymphocyte by MTT assay; detecting the lymphocytes cycle with the flow cytometer; and data processing. The method for detecting effects of Porphyra yezoensis polysaccharide PY-D2 on tumorresistance and immunity enhancement can reveal a significant role of the Porphyra yezoensis polysaccharide PY-D2 played in the treatment of tumors, meanwhile, the method also effectively promotes theapplication value of the Porphyra yezoensis polysaccharide PY-D2 in the treatment of tumors.

The invention belongs to the technical field of marine bioactive substancepreparation and specifically relates to a method for extracting phycoerythrin from dried porphyra yezoensis. The method specifically comprises the steps of smashing the dried porphyra yezoensis, screening by using a 80-mesh screen; mixing porphyra yezoensis powder with a phosphate buffer according to a certain ratio, and breaking the cells of the porphyra yezoensis by using a repetitive freeze-thaw method and an ultrasonic method; centrifuging a crude extract, collecting liquid supernatant, adding ammonium sulfate for sedimentation, and centrifuging again to obtain the phycoerythrin. The extracting method provided by the invention has the advantages of simple operation, mild preparation condition, environment friendliness, and high purity and good stability of the obtained phycoerythrin.

The invention discloses a culture method of pure-line porphyra yezoensis. The culture method comprises the following steps: 1) culturing porphyra yezoensis filament, and inducing the porphyra yezoensis filament to release conchospores; 2) culturing the conchospores; 3) cultivating the conchospore seedlings to grow monospore seedlings; 4) cultivating mother seedlings to continuously diffuse monospore seedlings; 5) cultivating the monospore seedlings to grow into large porphyra thalli, namely pure-line thalli consistent in genetic material; and 6) cultivating the pure-line thalli till sexual maturity to form carpospore cysts, and germinating the diffused carpospores to form filament, namely pure-line filament. According to the invention, all monospore seedlings are derived from the same monospore, homozygous filament can be obtained for sure, and the method is a fast and simple method for obtaining pure-line filament and is essential to the cultivation of good varieties of porphyra yezoensis.

The present invention discloses a method for developing a shellfish feed by utilizing waste porphyra yezoensis. The method comprises the following steps: S1, drying porphyra yezoensis: flatly spreading a single layer of porphyra yezoensis for natural sun-drying or drying the porphyra yezoensis in a 40-DEG C constant-temperature drying oven; S2, grinding porphyra yezoensis: putting the dried porphyra yezoensis into an ultrafine grinder, conducting grinding to obtain porphyra yezoensis dry powder to reach particle sizes of 300 meshes or above and filter feeding property within 50 microns; and S3, conducting fermenting: adding distilled water, a strain JSOU-1 and glucose into the treated porphyra yezoensis dry powder, and conducting stirring, uniform mixing, sealing and fermenting. The used fermentation method is solid-state fermentation, addition amount of water in the solid-state fermentation is small, a matrix is high in water insolubility and suitable for rapid propagation of probiotics, a large number of probiotics are good in activity of produced enzymes and high in fermentation capacity; and the solid-state fermentation has simple requirements on devices and low in initial investment, and besides, the method is easy to operate; and after the solid-state fermentation is finished, a sealed container can be repeatedly utilized without emission and pollution.

The invention relates to preparation of low molecular weight polysaccharides of Porphyra yezoensis and application of the low molecular weight polysaccharides of Porphyra yezoensis in resisting humancervical cancer cell tumors, and belongs to the field of biochemistry. The preparation is technically characterized in that macromolecular polysaccharides of Porphyra yezoensis are extracted by hot water boiling process first and are degraded into small molecular polysaccharides by gamma-ray radiation; the small molecular polysaccharides are mainly applied to resisting human cervical cancer cell tumors. The preparation method provided herein employs simple extraction process and is suitable for industrial production; the polysaccharides of Porphyra yezoensis prepared via the preparation methodhave a good anti-tumor function, are free of cell toxicity and are good in safety and reliability.

The invention provides a rapid PCR detection method for pseudoalteromonas nigrifaciens. The rapid PCR detection method uses a pseudoalteromonas nigrifaciens gene as a detection target gene, and comprises the detection steps of primer synthesis, DNA extraction, PCR reaction, electrophoresis detection and result determination. The rapid PCR detection method is the first method for detecting the pathogen of agar green spot disease in China, and the detection accuracy is high. Porphyra yezoensis is artificially infected with the pseudoalteromonas nigrifaciens, three pairs of specific primers are used for detecting the infected agar respectively, and the positive detection rate is 100%. The detection sensitivity is high, and the three pairs of primers can be used for specifically detecting thepseudoalteromonas nigrifaciens, and the detection sensitivity is 37 CFU, 3.7 CFU and 37 CFU of bacteria and 2.37pg, 23.7fg and 2.37 pg of DNA, respectively.

The invention provides application of a porphyra yezoensis shell conchocelis shell-dissolving agent. A Perenyi's solution is adopted as the shell-dissolving agent, and after porphyra yezoensis shell conchocelis is soaked with the Perenyi's solution, pathological observation of infected tissue is conducted. Compared with the prior art, the application has the advantages that the Perenyi's solutionis determined as the optimal shell-dissolving agent, when conchocelis is scraped after the porphyra yezoensis shell conchocelis is soaked with the Perenyi's solution for 90 seconds as the optimal acting time, the damage is low, and the observation effect is good.

The invention discloses a preparation method of a deep sea fish extract capable of prolonging the brain cell life. The preparation method comprises the following steps: step-by-step enzymatic hydrolysis, micro-filtration, secondary ultra-filtration and nano-filtration, macro-porous resin adsorption and eluting, freezing and drying. The preparation method disclosed by the invention has the benefits that the preparation method is simple, detailed data is provided for each step, the reproducibility is strong, and the suitability for large-scale production is realized; as a large number of restriction enzyme cutting sites of porphyra yezoensis proteases is on deep sea fish proteins, after enzymatic hydrolysis, peptide chains of different lengths can be obtained, and the deep sea fish extract capable of prolonging the brain cell life can be obtained through enzymatic hydrolysis with flavor proteinase. The obtained deep sea fish extract can inhibit the production of endogenous endonucleases and delay the DNA degradation of cell chromosomes, thereby delaying the expression of apoptosis procedures in neuronal cells and effectively prolonging the brain cell life.

The invention discloses a preparation method of hairtail active extract for repairing bone joints; the preparation method comprises the steps of enzyme complex hydrolysis, multistage membrane filtration, nanofiltration, HPLC (high-performance liquid chromatography) eluting and lyophilizing; the step of enzyme complex hydrolysis includes enzymatically hydrolyzing hairtail flesh homogenate via Bacillus marinus alkali-producing proteinase and Porphyra yezoensis proteinase, and performing ultrasonic-assisted enzymatic hydrolysis. The preparation method has the advantages that marine organism proteins are greatly differed from terrestrial life proteins in both amino acid composition and amino acid sequence and hairtail proteins can be enzymatically hydrolyzed via the Bacillus marinus alkali-producing proteinase and Porphyra yezoensis proteinase to obtain mass active peptides; the obtained hairtail active extract can promote the generation of cartilage cells, joint synovial fluid can be massively induced and supplemented, the generation of collagen matrix can be effectively promoted, regeneration of connective tissue cells is stimulated, and the reconstruction and recovery of soft tissues are accelerated accordingly.

The invention relates to an agamogony breeding method of porphyra yezoensis. The agamogony breeding method comprises the following steps: selecting single plant porphyra yezoensis thallus, of which the surface is washed, dried in the shade, and frozen, of a required character expression according to a breeding aim; taking out, and washing the frozen thallus again; separating the frozen thallus for cutting microchip tissue; carrying out static cultivation for the microchip tissue in a PES culture solution or the culture solution of adding N and P into boiled seawater under the condition of weak light and 15 to 18 degrees centigrade to acquire germplasm mitoplast. According to the agamogony breeding method, the germplasm mitoplast is formed by the object tissue in a way of agamogony but not gamogenesis; besides, the germplasm mitoplast can be stored as a provenance for years, and can be multiplied for using anytime; the time for purifying and breeding germplasm repeatedly in a conventional porphyra breeding process is made up and shortened, and the condition and the possibility are created for acquiring more porphyra yezoensis pure line germplasm provided with excellent characters.

The invention discloses a preparation method of porphyra protein antibacterial peptide, and belongs to the field of natural active material extraction. The method comprises the following steps: with porphyra yezoensis as a raw material, drying and crushing, performing ultrasonic crushing and ammonium sulfate protein precipitation on the raw material, enzymatically hydrolyze the porphyra yezoensis raw material by using papain under a specific condition to obtain a crude product antibacterial peptide, separating and purifying the crude product through chromatographic processes such as ion-exchange column chromatography and G-25 gel chromatography to obtain the porphyra protein antibacterial peptide. The porphyra protein antibacterial peptide molecular weight is mainly distributed at 1000Da and lower, and the optimum effect is that the pH is 6.5, and the heat stability is good; the porphyra protein antibacterial peptide has the effect on gram-negative bacteria and gram-positive bacteria, and has a stronger inhibition effect on the gram-positive bacteria, the antibacterial effect is achieved through the destructive effect on the cell membrane. The porphyra protein antibacterial peptide is small in molecular weight, good in antibacterial activity, and good in application prospect in the fields of preparing antibacterial medicines and food preservatives.