Application of porphyra yezoensis shell conchocelis shell-dissolving agent

A technology for shell filaments and striped laver, which is applied in the fields of development and disease of laver culture, and can solve the problems of difficulty in observing laver shell filaments and the like

Inactive Publication Date: 2019-10-11
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problem of difficulty in observing the filaments of Porphyra zebra sh

Method used

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  • Application of porphyra yezoensis shell conchocelis shell-dissolving agent
  • Application of porphyra yezoensis shell conchocelis shell-dissolving agent
  • Application of porphyra yezoensis shell conchocelis shell-dissolving agent

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Embodiment 1

[0033] Preparation of Perani liquid:

[0034] 10% nitric acid 200ml, 95% alcohol 150ml, 0.5% chromic acid 150ml.

[0035] Porphyra zebra filament culture conditions:

[0036] The temperature is 20°C, the salinity is 28‰, the light intensity is 20μmol / m2s, the photoperiod L:D=12:12, and the PES seawater is changed every 3 days.

[0037] Effects of shell filaments soaked in shell-dissolving agent for different times on the scraping effect:

[0038] After crushing the laver shell filaments, pick out pieces of similar size (less than 1cm×1cm), blot the water on the surface and completely immerse them in the Perani solution for 60s, 75s, 90s, 105s, 2min, 3min, 4min and 5min; 2-fold diluted Perani solution for 2min, 3min, 4min and 5min; 4-fold diluted Perani solution for 5min; 7%, 10%, 15% and 20% acetic acid seawater for 8min and 16min, and immediately washed with seawater for 3 The second time, use a scraper to gently scrape the algae filaments on the surface, observe their int...

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Abstract

The invention provides application of a porphyra yezoensis shell conchocelis shell-dissolving agent. A Perenyi's solution is adopted as the shell-dissolving agent, and after porphyra yezoensis shell conchocelis is soaked with the Perenyi's solution, pathological observation of infected tissue is conducted. Compared with the prior art, the application has the advantages that the Perenyi's solutionis determined as the optimal shell-dissolving agent, when conchocelis is scraped after the porphyra yezoensis shell conchocelis is soaked with the Perenyi's solution for 90 seconds as the optimal acting time, the damage is low, and the observation effect is good.

Description

technical field [0001] The invention relates to the fields of development and disease of laver culture, in particular to a method for observing laver shell filaments. Background technique [0002] Seaweed farming is an important part of aquaculture in my country, and its aquaculture output and area rank first in the world. Because of its high nutritional value, it is loved by people all over the world. Laver grows in the intertidal zone and can absorb a large amount of nitrogen and phosphorus in seawater, which has very important ecological significance. The life cycle of Porphyra can be divided into diploid filamentous stage and haploid thallus stage. Among them, the filaments can dissolve calcium, drill into the calcium-rich shells on the surface, and form shell filaments to survive the high temperature in summer. The shell filaments mature and swell in autumn and release conchospores. The amount of release and the degree of attachment directly affect the smooth progres...

Claims

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Application Information

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IPC IPC(8): A01G33/00G01N1/30G01N21/64G01N21/76G01N23/2251
CPCA01G33/00G01N1/30G01N21/6428G01N21/763G01N23/2251G01N2021/6432Y02A40/80
Inventor 莫照兰李杰杨慧超
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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