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Preparation method of deep sea fish extract capable of prolonging brain cell life

A technology of deep-sea fish and brain cells, which is applied in the field of preparation of deep-sea fish extracts, can solve the problems of low purity of complex polypeptides, inability to develop marine proteins, and low extraction rate, and achieve simple preparation methods, delay DNA degradation, and reproducibility strong effect

Inactive Publication Date: 2017-10-13
兰溪市捷喜食品加工技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of the compound polypeptide prepared by the above preparation method is not high, and the loss in the preparation process is large, and the extraction rate is not high
All proteases use terrestrial proteases, which cannot be well developed for marine proteins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of the deep-sea fish extract for prolonging the lifespan of brain cells comprises step-by-step enzymatic hydrolysis, microfiltration, secondary ultrafiltration and nanofiltration, macroporous resin adsorption elution and freeze-drying. The obtained deep-sea fish extract can inhibit the production of endogenous endonucleases, delay the DNA degradation of cell chromosomes, thereby delaying the expression of apoptosis programs in neuron cells and effectively prolonging their lifespan. The step-by-step enzymatic hydrolysis step is to add Porphyra cerevisiae protease and active short peptide to enzymolyze the saury surimi, adjust the pH and then add flavor protease to enzymolyze to obtain an enzymolyzed solution. There are a large number of enzymatic cleavage sites of Porphyra zebra protease on the protein of saury. After enzymatic hydrolysis, peptide chains of different lengths can be obtained, and then compound polypeptides that can effectively prolon...

Embodiment 2

[0030] The preparation method of the deep-sea fish extract for prolonging the lifespan of brain cells comprises the following steps:

[0031] 1) Step-by-step enzymatic hydrolysis: Add 25 times the volume of water to cod surimi, stir well, and then add 6wt‰ Porphyra zebra protease and 10% active short peptide enzymolysis of Porphyra zebra protease mass. The amino acid sequence of the active short peptide is SCASVCKARCRACRRGCGSVFCRCLRC. The enzymolysis temperature is 30°C, the pH is 8.0, the enzymolysis time is 32 minutes, and the enzyme is inactivated in a boiling water bath for 3 minutes after the enzymolysis is completed. After adjusting the pH, add 3wt% flavor protease for enzymatic hydrolysis. The enzymolysis pH is 6.5, the enzymolysis temperature is 55°C, and the enzymolysis time is 18 minutes. After the enzymolysis is completed, the enzyme is quenched in a boiling water bath for 3 minutes to obtain an enzymolysis solution;

[0032] 2) Microfiltration: The filtrate was m...

Embodiment 3

[0037] The preparation method of the deep-sea fish extract for prolonging the lifespan of brain cells comprises the following steps:

[0038] 1) Step-by-step enzymatic hydrolysis: add 27 times the volume of water to cod surimi, stir well, and then add 7wt‰ Porphyra zebra protease and 15% active short peptide enzymolysis of Porphyra zebra protease mass. The amino acid sequence of the active short peptide is SCASVCKARCRACRRGCGSVFCRCLRC. The enzymolysis temperature is 30°C, the pH is 8.0, the enzymolysis time is 32 minutes, and the enzyme is inactivated in a boiling water bath for 3 minutes after the enzymolysis is completed. After adjusting the pH, add 3wt% flavor protease for enzymatic hydrolysis. The enzymolysis pH is 6.5, the enzymolysis temperature is 55°C, and the enzymolysis time is 18 minutes. After the enzymolysis is completed, the enzyme is quenched in a boiling water bath for 3 minutes to obtain an enzymolysis solution;

[0039] 2) Microfiltration: The filtrate was m...

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Abstract

The invention discloses a preparation method of a deep sea fish extract capable of prolonging the brain cell life. The preparation method comprises the following steps: step-by-step enzymatic hydrolysis, micro-filtration, secondary ultra-filtration and nano-filtration, macro-porous resin adsorption and eluting, freezing and drying. The preparation method disclosed by the invention has the benefits that the preparation method is simple, detailed data is provided for each step, the reproducibility is strong, and the suitability for large-scale production is realized; as a large number of restriction enzyme cutting sites of porphyra yezoensis proteases is on deep sea fish proteins, after enzymatic hydrolysis, peptide chains of different lengths can be obtained, and the deep sea fish extract capable of prolonging the brain cell life can be obtained through enzymatic hydrolysis with flavor proteinase. The obtained deep sea fish extract can inhibit the production of endogenous endonucleases and delay the DNA degradation of cell chromosomes, thereby delaying the expression of apoptosis procedures in neuronal cells and effectively prolonging the brain cell life.

Description

technical field [0001] The invention relates to the field of health care products and medicines, in particular to a preparation method of deep-sea fish extract for prolonging the lifespan of brain cells. Background technique [0002] Peptides have only been paid attention to since the beginning of the 20th century. Studies have found that they are different from proteins and amino acids, and have the effect of drugs. It was not until the 1990s that there was considerable development. Under the guidance of constantly updated technologies, the scope of application of polypeptides became wider and wider, and their functions became more and more important. Only then did they enter our lives and occupy an important role in our lives. The share of peptide drugs is increasing, high efficiency, low toxicity and strong specificity have unique advantages different from other drugs, which are constantly affecting our lives. Moreover, the potential of peptides is huge, which is another...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16A61P25/00
CPCC12P21/06C07K1/16C07K1/34C07K1/36
Inventor 张庆吉
Owner 兰溪市捷喜食品加工技术有限公司
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