Method for measuring ascorbic acid content in porphyra yezoensis by high performance liquid chromatography

A high-performance liquid chromatography, a technology for laver striae, which is applied in the field of high-performance liquid chromatography for determining the content of ascorbic acid in laver, can solve problems such as less research on ascorbic acid, and achieve the effects of simple method, accurate detection, and good analysis effect.

Inactive Publication Date: 2012-06-20
ANHUI WAYEE SCI & TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few studies on the content of ascorbic acid in aquatic products, and some literatures have done a preliminary research on the content of ascorbic acid by titration method

Method used

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  • Method for measuring ascorbic acid content in porphyra yezoensis by high performance liquid chromatography
  • Method for measuring ascorbic acid content in porphyra yezoensis by high performance liquid chromatography
  • Method for measuring ascorbic acid content in porphyra yezoensis by high performance liquid chromatography

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Experimental program
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Embodiment 1

[0015] Embodiment 1: the method for the determination of ascorbic acid content in Porphyra variegata by high performance liquid chromatography is characterized in that comprising the following steps:

[0016] (1) Preparation and extraction of ascorbic acid in Porphyra variegata: Take some fresh Porphyra variegata, add 0.01mol / L potassium dihydrogen phosphate solution, homogenate, filter through a 0.20-0.24 μm microporous membrane, and wait for the test;

[0017] (2) prepare the standard solution of ascorbic acid, and make standard curve;

[0018] (3) Determination of ascorbic acid content in Porphyra variegata by HPLC.

[0019] The method for determining the content of ascorbic acid in Porphyra variegata by the described high-performance liquid chromatography is characterized in that: the chromatographic conditions of the described high-performance liquid chromatography are: chromatographic column: AQ-C18, 250×4.6mm, 5 μm; mobile phase : 0.01mol / L potassium dihydrogen phospha...

Embodiment 2

[0020] Embodiment 2: concrete experimental scheme of the present invention:

[0021] 1 Instruments and reagents

[0022] 1.1 Instruments:

[0023] Wanyi LC3000B high performance liquid chromatography equipped with UV3000 ultraviolet detector and LCS3000Q liquid chromatography workstation; homogenizer. AQ-C18, 250×4.6mm, 5μm chromatographic column.

[0024] 1.2 Material reagents:

[0025] Ascorbic acid standard (purity > 98%); fresh Porphyra variegata (collected from the Yellow Sea); methanol (HPLC grade); ultrapure water; potassium dihydrogen phosphate (analytical pure); 0.22 μm microporous membrane.

[0026] Mobile phase: Prepare a 0.01mol / L potassium dihydrogen phosphate solution, adjust the pH to 2.5 with phosphoric acid, mix the above solution with methanol at a volume ratio of 95:5 to make a mobile phase.

[0027] Ascorbic acid mother solution: Weigh 50 mg of ascorbic acid, dissolve it with 0.01 mol / L potassium dihydrogen phosphate solution (pH 2.5) and set the volume...

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Abstract

The invention discloses a method for measuring the ascorbic acid content in porphyra yezoensis by high performance liquid chromatography. In the method, the reverse high performance liquid chromatography is adopted; the chromatographic conditions of the reverse high performance liquid chromatography are that: a chromatographic column is a hydrophilic column (AQ) taking octadecyl silane as a filler; the chromatographic column can well retain substances with relatively high polarities, and take pure water as a mobile phase; a chromatographic mobile phase is 0.01 mol / L monopotassium phosphate (pH2.5)-methanol(95: 5, V / V); a detector is an ultraviolet detector with the detection wavelength of 265 nanometers; the flow speed is 1 ml / min; the sample inlet volume is 20 mu L; and the temperature of the column is 25 DEG C. According to the method, the ascorbic acid content in the porphyra yezoensis can be measured accurately and conveniently; and the method has the characteristics of high sensitivity and high reproducibility, and is easy to operate.

Description

technical field [0001] The invention relates to a chromatographic detection method, in particular to a method for measuring ascorbic acid content in Porphyra zebra by high performance liquid chromatography. Background technique [0002] High-performance liquid chromatography is a widely used method in the field of instrumental analysis. It achieves separation through various interactions between analytes and chromatographic fillers and uses the physical or chemical properties of analytes to achieve high-precision qualitative and quantitative analysis. At present, it is widely used in chemistry, chemical industry, polymer, biology, food, medicine and other fields. [0003] Ascorbic acid is a water-soluble vitamin that is abundant in fruits and vegetables. It plays a regulatory role in redox metabolic reactions, and its lack can cause scurvy. However, there are few studies on the content of ascorbic acid in aquatic products, and some literatures have made a preliminary explo...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 裴波尹杨青
Owner ANHUI WAYEE SCI & TECH CO LTD
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