Preparation method of hairtail active extract for repairing bone joints

Abstract

The invention discloses a preparation method of hairtail active extract for repairing bone joints; the preparation method comprises the steps of enzyme complex hydrolysis, multistage membrane filtration, nanofiltration, HPLC (high-performance liquid chromatography) eluting and lyophilizing; the step of enzyme complex hydrolysis includes enzymatically hydrolyzing hairtail flesh homogenate via Bacillus marinus alkali-producing proteinase and Porphyra yezoensis proteinase, and performing ultrasonic-assisted enzymatic hydrolysis. The preparation method has the advantages that marine organism proteins are greatly differed from terrestrial life proteins in both amino acid composition and amino acid sequence and hairtail proteins can be enzymatically hydrolyzed via the Bacillus marinus alkali-producing proteinase and Porphyra yezoensis proteinase to obtain mass active peptides; the obtained hairtail active extract can promote the generation of cartilage cells, joint synovial fluid can be massively induced and supplemented, the generation of collagen matrix can be effectively promoted, regeneration of connective tissue cells is stimulated, and the reconstruction and recovery of soft tissues are accelerated accordingly.

Description

technical field [0001] The invention relates to the technical field of preparation of health care products, in particular to a method for preparing an active hairtail extract for bone and joint repair. Background technique [0002] Peptides have only been paid attention to since the beginning of the 20th century. Studies have found that they are different from proteins and amino acids, and have the effect of drugs. It was not until the 1990s that there was considerable development. Under the guidance of constantly updated technologies, the scope of application of polypeptides became wider and wider, and their functions became more and more important. Only then did they enter our lives and occupy an important role in our lives. The share of peptide drugs is increasing, high efficiency, low toxicity and strong specificity have unique advantages different from other drugs, which are constantly affecting our lives. Moreover, the potential of peptides is huge, which is another m...

AI Technical Summary

Problems solved by technology

Marine biological proteins are very different from terrestrial biological proteins in terms of amino acid composition and amino acid sequence. The above method uses protease extracted from land to enzymatically hydrolyze marine organisms, and the enzymatic hydrolysis efficiency is low.