Molecular marker of Pyrus communis L. fruit titratable acid content
A technology of molecular markers and acid titration, applied in the field of molecular genetics and breeding, can solve problems such as lack of development, achieve the effects of improving accuracy, improving accuracy and efficiency, and speeding up progress
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Embodiment 1
[0023] a) Using the hybrid combination of two pear varieties 'August Red' and 'Dangshansuli' in my country, 97 F 1 group.
[0024] b) Analyze the two parents and their F 1 Population, statistical analysis of genetic types of polymorphic sites between parents in offspring populations. A total of 732 polymorphic marker loci, including 23 SSRs, S gene loci, 226 SRAPs, and 482 AFLPs, were finally obtained, and then linkage analysis was performed on them using Jionmap3.0 mapping software to construct a table containing Genetic linkage map of 240 polymorphic markers in 'August Red' pear.
[0025] Using SSR, SRAP, and AFLP molecular marker methods, the 'August Red' and 'Dangshan Suli' and their offspring were analyzed, and the genetic types of the polymorphic sites between the parents were statistically analyzed in the offspring population.
[0026] For the experimental procedure of SSR labeling, refer to the method of Yomamoto T. et al. (Euphytica, 2002, 124:129-137); for the exp...
Embodiment 2
[0046] Using the screened molecular markers linked to the main QTL for titratable acid content in fruit of ‘Augusthong’, 97 strains of F in ‘Augusthong’ and ‘Dangshansuli’ 1 Preliminary testing was carried out on the hybrid progenies to test the practical value of this method in molecular marker-assisted selection of titratable acid content in pear fruit. Use the genomic DNA of the 97 offspring and AFLP selective amplification primer combination E-AAC / M-CAG to carry out PCR reaction, after electrophoresis according to EAACMCAG-850m * Whether there is a corresponding marker to determine whether there is a corresponding marker. If there is a marker, it means that the fruit titratable acid content of the plant is high, and if it does not exist, it means low. The results are verified.
[0047] The results showed that the 97 F 1 Among the hybrid offspring, a total of 63 strains showed EAACMCAG-850m in DNA amplification results * Among these 64 strains, there are 35 strains whose...
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