Method for establishing DNA (deoxyribonucleic acid) fingerprint of green vegetable hybrid and application thereof

Abstract

The invention discloses a method for establishing a DNA (deoxyribonucleic acid) fingerprint of green vegetable hybrid and parents thereof, and application thereof. The method comprises the following steps: 1) extracting and purifying a green vegetable DNA; 2) carrying out RAPD (random amplified polymorphic DNA), ISSR (inter-simple sequence repeat) and SRAP (sequence-related amplified polymorphism) analysis by using the acquired high-purity green vegetable DNA as a template; and 3) choosing a typical polymorphism amplification strip to establish the DNA fingerprint of tested material, wherein both the green vegetable hybrid and the parents thereof in the DNA fingerprint have specific DNA fingerprints which can be distinguished. The DNA fingerprint disclosed by the invention is presented in a graphic mode, so the DNA fingerprint is visualized and can be easily understood. The graphic mode of the DNA fingerprint can be converted into a digital code presenting mode, thereby being conveniently read and analyzed by a computer. By using the method disclosed by the invention, the seed purity of the green vegetable hybrid can be identified from the heredity nature, the result is accurate and reliable, and the detection can be quickly performed.

Description

technical field [0001] The invention relates to identification technology of cabbage seeds, in particular to a method for establishing DNA fingerprints of hybrid cabbage and its application. Background technique [0002] At present, many green vegetables (Brassic campestris L. ssp. Chinensis (L.) Makino) varieties popularized and applied in my country are the first generation of hybrids. The first generation of hybrids can synthesize many excellent characters of the parents, and the economic benefits are better. [0003] However, in the process of seed production, due to improper management measures and the defects of self-incompatibility itself, self-seeds, ie false hybrids, are often produced, resulting in a decrease in seed purity. Therefore, improving the purity of hybrids is an important topic in the field of heterosis utilization research. At present, there are roughly three methods for variety identification and seed purity analysis, namely field morphology, biochem...

AI Technical Summary

Problems solved by technology

The traditional field identification method uses the color, shape and other agronomic traits of seeds, seedlings, leaves, flowers, fruits, pollen and other tissues and organs as the observation indicators for identification. Although it is a relatively feasible method in production practice, field identification requires Additional land occupation, long period, high cost, not only limited by seasons, but also the performance of many traits is affected by cultivation measures and environmental factors, and the observation experience of the appraiser also restricts the accuracy of appraisal

The biggest defect of Daejeon identification is that it cannot provide timely information on the purity of seeds, and cannot provide information on the quality of seeds before a large number of seeds are traded, and cannot play a role in pre-supervising the quality of seeds

Biochemical identification methods include protein electrophoresis and isoenzyme electrophoresis, which have been increasingly widely used, but they cannot fully display the polymorphisms between varieties, and it is difficult to effectively distinguish varieties with close relatives

Images