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A genotyping detection kit for yak parentage identification and individual identification

A detection kit and paternity testing technology, which is applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., and can solve the problems of failing to detect yak STR sites

Inactive Publication Date: 2021-03-02
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the STR marker primers of some common cattle can be used to amplify the corresponding loci of yaks, after all, there are certain differences in gene sequences between yaks and common cattle, and the direct use of common cattle primers often cannot reach the yak STR loci. The best effect of detection

Method used

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  • A genotyping detection kit for yak parentage identification and individual identification
  • A genotyping detection kit for yak parentage identification and individual identification
  • A genotyping detection kit for yak parentage identification and individual identification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Screening and determination of STR markers for paternity identification of yaks:

[0098] 1. Selection and grouping of STR marker sites

[0099] 267 unrelated yaks were selected, and their venous blood DNA was extracted and tested. According to relevant reports of literature research, 36 STR loci with no linkage relationship between common cattle and yak and high polymorphic information content were selected, and sequence analysis was performed on the primers reported in the literature and the corresponding loci in yak. Inappropriate primers such as primer dimers and large differences in annealing temperature were redesigned. In addition, four STR sites (CH28AC, CHX2TG, CHX3TA, CHX6AT) were searched on the yak genome (see Table 7), and primers were designed for each STR site and the sequences at its two ends to amplify the corresponding sites and perform electrophoresis detection. .

[0100] Table 7 Information of 40 pairs of yak STR common primers

[0101]

[01...

Embodiment 2

[0123] Application of 14 STR markers in paternity identification of yak triplets:

[0124] A pair of female yaks and offspring yaks with clear mother-child relationship are selected from the parent-child group of yaks, and the semen of two male yaks suspected to be fathers are selected, and the parent-child relationship is tested with the kit of the present invention.

[0125] 1. Sample:

[0126] (1) The number of the offspring yak is B1007004, and the blood sample tube number is V111417;

[0127] (2) The number of the female yak is B3916076, and the blood sample tube number is Y72372214;

[0128] (3) The number of candidate male Yak 1 is C1011029, and the number of frozen semen is 237246;

[0129] (4) The number of candidate male Yak 2 is C2311097, and the number of frozen semen is 237247.

[0130] 2. Extract the DNA of each blood sample and semen sample, and use the kit in the present invention to perform fluorescent primer amplification and capillary electrophoresis dete...

Embodiment 3

[0139] Application of 14 STR markers in yak semen identification:

[0140] The two batches of preserved frozen semen are suspiciously collected from the same yak bull, and the blood samples of the yak bull and the two batches of frozen semen samples are taken, and the individual identification test is carried out with the kit of the present invention.

[0141] 1. Sample:

[0142] (1) The number of the yak bull is C0811069,

[0143] (2) The label of frozen semen 1 is 237369;

[0144] (3) The number of frozen semen 2 is 237381.

[0145] 2. Extract the genomic DNA of the yak bull blood sample and two batches of yak semen samples, and use the kit in the present invention to perform fluorescent primer amplification and capillary electrophoresis detection on each sample.

[0146] 3. Inspection and results

[0147] According to the difference in fluorescence signal of each amplification product in the above capillary electrophoresis, the length of the amplification product of the...

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Abstract

The invention discloses a compound PCR amplification system and a detection kit for genotyping of the STR marker detection system for paternity identification and individual identification of yaks. The primers have fluorescent markers and can amplify 14 STR marker sites of yaks. These markers are highly polymorphic, unlinked among the markers, and have appropriate fragment sizes; the STR detection system obtains the genotyping results of 14 STR loci through the amplification and electrophoresis detection of the 14 pairs of primers; The paternity test and individual identification of yaks were carried out based on the type results and the gene frequency of each STR in yaks. The scheme of the present invention can effectively perform paternity identification and individual identification of yaks by comprehensively analyzing the typing results of STR loci, thereby improving the accuracy of pedigree in yak breeding work, speeding up the breeding process, and has good application prospects and economic value.

Description

technical field [0001] The invention relates to the technical field of in vitro detection of animal nucleic acid, in particular to a compound PCR amplification system and a detection kit for genotyping of a STR marker detection system for yak paternity identification and individual identification. Background technique [0002] Pedigree information plays an important role in yak breeding. Wrong pedigree will greatly reduce the accuracy of genetic evaluation, affect the effect of selection and matching, reduce the genetic progress of the population, and bring huge economic losses to the yak industry. However, in actual breeding production, pedigree record errors are unavoidable, and it is affected by many factors, such as breeding records, calving records, human errors in pedigree entry and sorting, especially in the state of extensive free-range grazing , it is difficult to determine the father, or even the mother, of a calf after birth. [0003] Short tandem repeats (Short...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156C12Q2600/16
Inventor 裴杰郭宪褚敏包鹏甲梁春年丁学智王宏博吴晓云阎萍
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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