89 results about "Semen sample" patented technology

A method for increasing the percentage of mammalian offspring of either sex which comprises contacting a semen sample with an antibody specific for the spermatozoa determinative of one sex and separating said spermatozoa from spermatozoa determinative of the other sex, said antibody being bound to a non-porous magnetic bead support having a diameter of 0.1 to 2 microns.

A method for increasing the percentage of mammalian offspring of either sex which comprises contacting a semen sample with an antibody specific for the spermatozoa determinative of one sex and separating said spermatozoa from spermatozoa determinative of the other sex, said antibody being bound to a non-porous magnetic bead support having a diameter of 0.1 to 2 microns.

A jacketed container for holding a semen sample from a mammalian donor at a predetermined temperature for a predetermined period of time prior to incubation to enhance the probability of obtaining offspring of a selected sex through artificial insemination is disclosed. In one embodiment, the semen sample is collected and insulated by a high-heat capacity material that has been preconditioned to a temperature between about 30° C. to about 40° C. before the semen is cooled to a temperature of about 12° C. In a second embodiment, the semen sample is collected and insulated by a high-heat capacity material that has been preconditioned to a temperature between about 4° C. to about 20° C. before the semen is cooled to a temperature of about 12° C.

A method and device for assaying sperm motility in a forward direction and density of active sperm in a semen sample are disclosed. The device includes a microfluidics structure having a sample reservoir, a downstream collection region and a microchannel extending therebetween. The microchannel is dimensioned to confine sample sperm to single-direction movement within the channel, such that sperm in a semen sample placed in the sample reservoir enter and migrate along the microchannel toward and into the collection region. Also included is a detector for detecting the presence of labeled sperm in the microchannel or collection region, and an electronics unit operatively connected to the detector for (i) receiving detector signals, (ii) based on the detector signals received, determining sperm motility and density in the sperm sample, and (iii) displaying information related to sperm motility and density.

Disclosed are processes and kits for rapid nucleic acid extraction from a nucleic acid-containing material, such as a bone, tooth or semen sample. For bone and tooth process involves providing the nucleic acid-containing material in a form suitable for nucleic acid extraction, adding a lysis buffer to the nucleic acid-containing material to obtain a mixture, mixing the mixture in a manner equivalent for about 30 seconds or longer and separating the mixture by centrifugation to obtain a liquid supernatant. The liquid supernatant contains the extracted nucleic acids which can be used for analysis including STR profiling by conventional or rapid DNA analysis. For semen the processes and kits involve applying an appropriate amount of sperm disruptive agent.

A method for measuring the total sperm concentration (TSC) in a sample is described and includes: (i) placing the sample in a transparent container between a synchronically pulsed light source and a photodetector; and (ii) measuring the optical absorbance of the sample in the range of 800-1000 nm, the TSC of the sample being proportional to the absorbance. Also described is a sampling device for use in optically analyzing a biological fluid, a method for measuring motile sperm concentration (MSC) in a semen sample, a method of determining the average velocity (AV) of sperm cells and a system for analyzing semen quality including means for measuring TSC, means for measuring MSC; and a video visualization system.

In a device to facilitate the collection of semen, a flange is provided for a semen cup, which extends inwardly from the wall of the cup and defines a central opening, thereby providing a peripheral flange to prevent semen from flowing back out and allowing the cup to be held in an inverted or angled orientation while providing the semen sample.

Materials and methods for the separation of X- and Y-chromosome bearing sperm, for example in a semen sample, are provided. The methods involve contacting the semen sample with a binding agent, such as an antibody, that specifically binds to an antigen that is specific for an X- or Y-chromosome. Kits for use in the methods are also provided.

The invention relates to a raman spectrum-based fast measurement method for a motile sperm deoxyribonucleic acid (DNA). The invention adopts the following technical scheme: collecting a semen sample by a test tube; naturally liquefying semen in a room-temperature environment; absorbing the liquefied semen and adding to a centrifugal tube containing density gradient centrifugate; absorbing upper seminal plasma after centrifuging, discarding, then adding a PBS buffer solution and centrifugally washing again; absorbing supernatant, discarding, adding the PBS buffer solution to prepare a sperm suspension, and incubating; attaching a metal bottom plate to a culture dish; adding the PBS buffer solution, and absorbing the incubated sperm suspension, soaking in the culture dish, wherein the sperm swims outside and contacts the metal bottom plate; setting a spectrum response range, integrating time and the excitation light wavelength, so as to obtain raman spectrum data from the head part of the sperm. The motile sperm swimming out from the pointed-end part of a sucker can be attached to the metal bottom plate by operation of a thin tube pipette, and the damage condition of the sperm DNA is evaluated by measuring the sperm of which the head is attached to the metal bottom plate.