52 results about "Motile sperm" patented technology

A kit for testing male fertility comprises a vessel, a base unit, a liquid supply containing liquid and two filters. The first filter is a sample separation filter which forms a hindrance to transmission of spermatozoa. The second filter of the kit is a spermatozoa detection filter comprising a reagent for identifying spermatozoa. Activation of the kit is prevented until a transport medium, such as the liquid, fills a gap allowing spermatozoa to transmit to a detection zone. The kit may be of one-piece construction and utilizes a thin piece of filter material to separate motile from non-motile spermatozoa.

Device (10, 110) for separating motile sperm from a sample (100, 200), comprises a vessel (15, 115) having an inlet port (20, 120), an outlet port (35, 135) which is initially closed, a medium (40, 140) into which motile sperm in the sample (100, 200) can migrate into the vessel (15, 115) via the inlet (20, 120), and an actuator (50, 150), the operation of which opens the outlet (35, 135), thereby allowing the medium (40, 140) to flow out of the vessel (15, 115) through the outlet (35, 135). The medium (40, 140) in the vessel (15, 115) is initially prevented from flowing through the outlet (35, 135), thus allowing an incubation period which allows motile sperm sufficient time to migrate from the sample (100, 200) into the medium (40, 140) before the medium (40, 140) leaves the vessel (35, 135). The device (10, 110) preferably comprises a spermatozoa detector in communication with the outlet. In devices for separating sperm, capillary flow takes place through non-fibrous material, such as the space between sheets (81a and 81b; 181a and 181b) of closely-juxtaposed material. A device for separating motile and non-motile sperm includes a temperature sensor, a heat source (70, 170) and, optionally, a temperature regulator.

Motile particles are sorted from non-motile particles in a microfluidic sorting device wherein a stream of sort fluid containing motile and non-motile particles is caused to flow adjacent a media stream in non-turbulent fashion through a sort channel, during which flow motile particles cross the interface between the adjacent flow streams, entering the media stream, and forming a motile particle-depleted sort stream. The sorting devices are easily and inexpensively fabricated and have numerous uses, in particular sorting of motile from non-motile sperm.

A novel sperm quality assessment device includes a main frame and a test pad. The test pad is encircled by the main frame and includes an exposed introductory portion. The test pad has greater hydrophilicity than the main frame, and includes an MTT reagent. Therefore, the activity pattern of the colored motile sperms can be used to estimate sperm quality, such as motility. The present device has the particular advantage of using paper to reduce manufacturing cost, and is also easy to use.

Device (10, 110) for separating motile sperm from a sample (100, 200), comprises a vessel (15, 115) having an inlet port (20, 120), an outlet port (35, 135) which is initially closed, a medium (40, 140) into which motile sperm in the sample (100, 200) can migrate into the vessel (15, 115) via the inlet (20, 120), and an actuator (50, 150), the operation of which opens the outlet (35, 135), thereby allowing the medium (40, 140) to flow out of the vessel (15, 115) through the outlet (35, 135). The medium (40, 140) in the vessel (15, 115) is initially prevented from flowing through the outlet (35, 135), thus allowing an incubation period which allows motile sperm sufficient time to migrate from the sample (100, 200) into the medium (40, 140) before the medium (40, 140) leaves the vessel (35, 135). The device (10, 110) preferably comprises a spermatozoa detector in communication with the outlet. In devices for separating sperm, capillary flow takes place through non-fibrous material, such as the space between sheets (81a and 81b; 181a and 181b) of closely-juxtaposed material. A device for separating motile and non-motile sperm includes a temperature sensor, a heat source (70, 170) and, optionally, a temperature regulator.

The invention relates to a preferential sperm freezing culture device which comprises freezing slide glass, a sperm tank, a sperm swimming channel, a collecting tank and a blocking piece. The volume of the sperm tank ranges from 50 microliters to 500 microliters, the volume of the collecting tank ranges from 5 microliters to 20 microliters, the width of the sperm swimming channel ranges from 10 micrometers to 30 micrometers, and the sperm swimming channel is connected with the sperm tank and the collecting tank. The preferential sperm freezing culture device has the advantages that the preferential sperm freezing culture device is applicable to screening preservation of frozen sperm, motile sperm can be automatically separated by the design of the sperm tank, the sperm swimming channel andthe collecting tank, operation amount is decreased, mechanical damage to the sperm caused by manual picking is avoided, the proportion of usable motile sperm is increased, the freezing slide glass ofall existing sperm can be completely replaced, the preferential sperm freezing culture device can be applied to the fields of normal sperm optimization sperm periodic IVF (in-vitro fertilization), ICSI (intracytoplasmic sperm injection) or artificial insemination, sperm collection of oligospermia and asthenospermia patients, surgical sperm extraction of azoospermatism patients, sperm collection and the like, and has a wide popularization and application prospect.

Staining mixtures comprising viable spermatozoa, a composition which regulates oxidation/reduction reactions intracellularly or extracellularly, and a DNA selective dye are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for staining sperm cells comprising the formation of a staining mixture are also disclosed.

The present invention relates to a system to analyse general diagnostic factors in cells and body fluids using a flow cytometer, and in particular to a system featuring a number of different fertility tests, in a simple, expedited format, in order to investigate factors affecting fertility, preferably in a semi or fully automated manner. Specifically, a preparative method has been developed to increase the success of in vitro fertilisation (I.V.F) and intrauterine insemination (I.U.I) in cases of immunoinfertility by removing sperm-bound antibodies from sperm cells. A special device has been designed to collect only motile sperm cells from semen samples. Thus, this invention provides improved methods for general diagnostic testing and infertility screening and enables gynecologists to obtain information from an infertile couple in a preliminary test, which until now has been time consuming and only possible to run in sophisticated laboratories.

The present invention addresses the problem of providing an agent for improving sperm-motility, in particular, an agent for improving forward-sperm-motility, for use on reduced-motility sperm in male infertility treatment. Prepared is a gaseous agent for improving sperm-motility consisting of a gas that contains hydrogen molecules in the amount of 1% (v/v) or higher, for example, a gas that contains hydrogen molecules in an amount of between 45 and 55% (v/v), or a liquid agent for improving sperm-motility consisting of a liquid such as physiological saline, a culture solution, or a buffer solution containing hydrogen molecules in the amount of 1% or higher of the saturation solubility thereof, for example, a liquid in which hydrogen molecules have been dissolved by bubbling. The gaseous agent for improving sperm-motility can improve the motility of sperm by contacting hydrogen gas and either semen or a dilution thereof with each other in a gaseous phase. The liquid agent for improving sperm-motility can improve the motility of sperm when used as a washing solution or diluent for semen.

The invention relates to an automatic sperm floating apparatus. The apparatus comprises a liquid inlet tube, a liquid outlet tube and a tube body, the upper end of the tube body has a conical shape, the top of the conical tube body is connected with the liquid outlet tube, and the liquid outlet tube is communicated with the internal of the tube body; the liquid inlet tube is fixed to a sidewall of the tube body, the bottom of the liquid inlet tube is communicated with the bottom of the tube body, and the upper end of the liquid inlet tube is provided with a tube cover; and the materials of the liquid inlet tube, the liquid outlet tube and the tube body are a deformable nontoxic transparent plastic. The apparatus has the advantages of reasonable structure design, no need of extra suction tubes for the addition of a culture liquid and a semen and the suction of a culture liquid containing motile sperms, material consumption saving, convenient and controllable operation, furthest reduction of the culture liquid pollution possibility, and guarantee of the cleanliness and purity of the motile sperms obtained through a floating method.

The invention discloses a semen film surface antibody immunity method testing agent and preparing and testing method. The semen film surface antibody immunity method testing agent comprises: 1.8um-2.2um immunity bead which can possesses human antibody IgG or IgA or IgM micromole rubber latex particle, the washing liquid which contents 0.1%-0.5% bonnie serum albumin and the scale dissolver which contents 4%-6% bonnie serum albumin. The agent can also comprise semen extracting liquid with high activity. The invention provides the method for preparing the semen film surface antibody immunity method testing agent.