36 results about "Sperm injection" patented technology

A microinjection device is provided that includes an injection element defining a longitudinal axis, and that further includes a motor. The injection element is rotatable about the longitudinal axis by the rotational motor. The injection element is for penetrating a target, such as a cell. A microinjection system is provided that includes the microinjection device and a control unit. The control unit is for controlling a rotational amplitude and a frequency of oscillation of the injection element. A method for penetrating a target to facilitate injecting material therein is provided that includes providing the material to an injection element, contacting the target with a distal end of the injection element, rotating the injection element about a longitudinal axis to form a hole in the target, and penetrating the target with the injection element via the hole formed in the target. A method for performing intra-cytoplasmic sperm injection is provided that includes providing a solution comprising sperm to an injection element, contacting an oocyte with a distal end of the injection element, rotating the injection element alternately clockwise and counterclockwise about a longitudinal axis to form a hole in the oocyte, penetrating the oocyte with the distal end of the injection element via the hole formed in the oocyte, and expelling the solution comprising sperm into the penetrated oocyte.

A method and device to conform with the glans penis to recover ejaculated semen completely, to prevent the loss of initial sperm rich epididymal fractions, to avoid the use of a condom for masturbation, to eliminate the multi-step transfers of semen following ejaculation that are common with current methods, and to provide a single device that contains a semen collecting chamber that fits onto a glans penis. The device provides a storage and measuring reservoir, an optional cap, and a vertical stand in one integrally formed module in a sterile pack. The device and method will have multiple uses in a variety of contexts including, but not limited thereto: in the diagnosis of infertility, in semen donation, in vitro fertilization (IVF) / intra-cytoplasmic sperm injection (ICSI) clinics, hospitals, forensic laboratories and research laboratories and will be included in kits intended for over-the-counter sperm testing devices

A device for transferring cells present in fluid media is provided which is especially useful for in vitro fertilization techniques and intracytoplasmic sperm injection procedures.

A method and device to conform with the glans penis to recover ejaculated semen completely, to prevent the loss of initial sperm rich epididymal fractions, to avoid the use of a condom for masturbation, to eliminate the multi-step transfers of semen following ejaculation that are common with current methods, and to provide a single device that contains a semen collecting chamber that fits onto a glans penis. The device provides a storage and measuring reservoir, an optional cap, and a vertical stand in one integrally formed module in a sterile pack. The device and method will have multiple uses in a variety of contexts including, but not limited thereto: in the diagnosis of infertility, in semen donation, in vitro fertilization (IVF) / intra-cytoplasmic sperm injection (ICSI) clinics, hospitals, forensic laboratories and research laboratories and will be included in kits intended for over-the-counter sperm testing devices.

An integrated automated system comprising a microfluidic cassette, and methods of use thereof, for intracytoplasmic sperm injection assisted fertilization. The microfluidic cassette and the integrated automated system provides a complete set-up of human gametes for assisted in vitro fertilization, including proper cell stage recognition, gamete propulsion via microfluidic currents, microinjection of a spermatozoon into an oocyte, and subsequent embryo culture and monitoring, thus allowing widespread distribution of in vitro insemination by favoring affordability.

The invention relates to a La-1-like peptide agent that increases sperm motility. It is non-toxic and it may be used to treat male infertility in humans, and also to increase fertility in animals. It may be used in artificial reproductive techniques such as in vitro fertilisation (IVF), including intra-cytoplasmic sperm injection (ICSI). The La-1 like peptide agent may also be used in artificial insemination techniques such as intra-cytoplasmic uterine injection (IUI).

The invention relates to a recombinant human granulosa cell enzyme solution and a preparation method of same and belongs to the technical field of artificial assisted reproduction. The recombinant human granulose cell enzyme solution is prepared by dissolving recombinant human granulosa cell enzyme dry powder, which is obtained by processing ovum, in a salt solution containing antibiotics and an indicator. In the invention, by means of a physiological work solution, which is prepared by means of non-animal-sourced recombinant bio-engineering high-tech hyaluronidase (a.k.a. recombinant human granulosa cell enzyme) dry powder, is 80 units (I.U.) and has functional available concentration, not only are granule cells surrounding the ovum effectively digested, which is beneficial to introcytoplasmic sperm injection (ICSI) technology of the ovum, but also possibility of animal-sourced pathogen and toxin are effectively excluded, thereby greatly improving safety of assisted reproduction.

The invention provides a micro fertilization method, which mainly comprises the following steps of: 1) taking fresh seminal fluid or frozen thawed seminal fluid; 2) taking fresh oocyte cells after the human chorionic gonadotropin (HCG) is injected for 14 to 18 hours; 3) respectively injecting the sperm in the fresh seminal fluid and the live sperm in the frozen thawed seminal fluid into the oocyte cells obtained in the second step to obtain injection oocyte; 4) activating the injection oocyte by using 5mumol / L ionomycin and 2mmol / L 6-dimethyaminopurine (6-DMAP) to obtain fertilized ovum; and 5) placing the fertilized ovum into culture liquid consisting of TCM199, 10 percent fetal bovine serum (FBS), 1.25 mM sodium pyruvate and 0.1 mM ethylene diamine tetraacetic acid (EDTA) to be cultured. The oocyte cell cytoplasm introcytoplasmic sperm injection method provided by the invention is simple and has the advantages that the operation is easy, and in addition, the large-scale production and popularization is convenient.

The invention relates to a La-1-like peptide agent that increases sperm motility. It is non-toxic and it may be used to treat male infertility in humans, and also to increase fertility in animals. Itmay be used in artificial reproductive techniques such as in vitro fertilisation (IVF), including intra-cytoplasmic sperm injection (ICSI). The La-1-like peptide agent may also be used in artificial insemination techniques such as intra-cytoplasmic uterine injection (IUI).

The invention relates to the field of assisted reproduction, and discloses a composition for promoting sperm activation and a sperm activation solution. The sperm activation solution comprises a basicculture solution and a composition, wherein the composition comprises 2-10mg / L of ATP sodium salt, 5-15 U / mL of heparin, 5-10 [mu]g / mL of spot adhesion protein, 1-2.5[mu]g / mL of allene oxide synthase, 4-4.5 [mu]g / mL of acetyl coenzyme A acetyltransferase 2, 0.9-1.1 mmol / L of phosphatidylserine, 5.5-10.5 [mu]g / mL of coenzyme Q10, 3.5-6.5 ng / mL of linolenic acid and 0.45-0.5 [mu]g / mL of recombinantheat shock protein HSPA8. The invention also discloses a preparation method for the sperm activation solution. According to the sperm activation solution, the time of sperm injection and ovum in-vitro operation can be greatly shortened; the fertilization rate and the blastocyst formation rate are increased; the pregnancy rate is directly or indirectly increased; meanwhile, the sperm activation solution is effectively suitable for activation and optimization of sperms in an oocyte intracytoplasmic single sperm microinjection technology (ICSI) and an in-vitro fertilization (IVF) process in an in-vitro fertilization assisted reproduction technology.

The present invention relates to a long acting biologically active luteinizing hormone (LH) compound comprising an LH agonist linked to a pharmaceutically acceptable molecule providing an in vivo plasma half-life of the LH agonist or LH compound which is increased substantially compared to the in vivo plasma half-life of an LH agonist administered in the same manner as the LH compound. The present invention relates to methods for controlled ovarian stimulation which can be used in conjunction with assisted reproduction technologies such as in vitro fertilisation, intra cytoplasmatic sperm injection, intra uterine insemination and in vitro maturation. In other aspects the invention relates to methods for inducing folliculogenesis and methods for providing luteal support for the corpora lutea.

The invention relates to a polyvinylpyrrolidone solution and a preparation method thereof, and belongs to the field of assisted reproductive technology (ART). The polyvinylpyrrolidone solution provided by the invention is prepared by adding taurine, serine, threonine, glycine, proline, chelating agent EDTA and polyvinylpyrrolidone into a salt solution. Not only the integrity of sperm plasma membranes can be effectively maintained, but also EDTA can chelate calcium ions, reduce the loss of calcium ions during self-motion removal of sperms, in order to improve the fertilization rate of sperm injection, and also provide a strong guarantee for the safety of assisted reproductive technology.

Co-injection of sperm heads and exogenous nucleic acid encoding the transgene into unfertilized mouse oocytes resulted in embryos expressing the transgene, reflecting the combination of nucleic acid and sperm heads before co-injection. Embryos obtained by co-injection were non-selectively transferred to surrogate mothers, producing offspring expressing the integrated transgene.

The invention discloses a method for carrying out ICSI (intracytoplasmic sperm injection) on a pig. The method for carrying out ICSI on the pig comprises the following steps: (1) hydrolyzing sperms in vitro animal semen with lipoidase or lipoidase solution, and collecting a hydrolysis product, so that the sperms used for preparing ICSI fertilized ova; (2) injecting the sperms used for preparing the ICSI fertilized ova into in vitro oocytes to obtain ICSI ova; and (3) carrying out electric activation on the ICSI ova and cultivating, so that the ICSI fertilized ova are obtained. The method for carrying out ICSI on the pig has the advantages that bad effects on depolymerization of pig sperms, formation of male pronucleus and later development due to addition of PVP can be avoided, and regions such as plasma membranes and perforatorium of the pig sperms can not be damaged, so that release of oocyte activation promoting factors from the pig subjected to ICSI operation can be facilitated, and the formation rate of the male pronucleus and the formation rate of blastocysts of embryos after fertilization are increased.

The invention belongs to the field of medicine, and discloses application of a histone H3K79 methyltransferase inhibitor in improving the development efficiency of animal round sperm injection embryos. The invention discloses the application of the histone H3K79 methyltransferase inhibitor in improving the development efficiency of the animal round sperm injection embryos for the first time. Compared with an existing round sperm injection technology, the application has the advantages that under the condition that the transgenic risk is not introduced, the blastocyst development rate of the round sperm injection embryos can be remarkably increased through the histone H3K79 methyltransferase inhibitor, a good round sperm injection embryo in-vitro culture system is established, and the feasibility of the round sperm injection technology is improved.

The invention relates to an automatic ICSI system capable of accurately identifying, which comprises a power supply module, a main control module, a sample optical observation module, a user interaction module, a sperm capturing unit, an ovum fixing unit and a single sperm injection unit, the sample optical observation module, the user interaction module, the sperm capturing unit, the ovum fixing unit and the single sperm injection unit are all electrically connected with the main control module, and the operation method of the system comprises three steps of sperm capturing, ovum fixing and single sperm injection. According to the automatic ICSI system capable of accurately identifying and the operation method, the system can replace manual work to realize automation, is stable and reliable in experimental structure, adopts an AI image system to identify healthy and high-quality sperms and intelligently identify the spindle body part of an ovum, can perform needle insertion and injection on the best-quality sperms through the most appropriate position, greatly reduces the damage to the ovum, and the probability of various fertilization failures or embryo development potential underground caused by human factors is reduced to the greatest extent.

The invention belongs to the field of medicines, and discloses application of a histone methyltransferase inhibitor to improvement of animal round sperm injection embryo development efficiency. The invention discloses the application of the histone methyltransferase inhibitor in improving the animal round sperm injection embryo development efficiency for the first time. Compared with an existing round sperm injection technology, the blastocyst development rate, implantation rate and living yield of the round sperm injection embryo of the animal can be remarkably improved through the histone methyltransferase inhibitor under the condition that transgenic risks are not introduced; in addition, the round sperms treated by the histone methyltransferase inhibitor can be injected to generate the live F0 generation to normally breed offspring, a good round sperm injection embryo in-vitro culture system is established, and the feasibility of the round sperm injection technology is improved.

The invention discloses the application of Apelin-APJ inhibitor in the treatment of blood-testis barrier damage. The inventors of the present invention found that the use of Apelin-APJ inhibitor can effectively promote the expression of genes related to the blood-testis barrier in cells, ensure the integrity of the blood-testis barrier, increase the blastocyst rate of intracytoplasmic sperm injection in mice, and reduce zygotic embryo arrest, Therefore, it can be used for related problems such as reproductive system damage caused by chronic diseases, and has extremely high application value and clinical significance. Moreover, the Apelin-APJ inhibitor in the present invention has no obvious toxic and side effects within a dosage of 0.1-100 μM, and can be safely used for related purposes such as drug preparation.

The invention relates to a bovine sperm treatment method and a bovine intracytoplasmic sperm injection method, and belongs to the technical field of intracytoplasmic sperm injection. According to the bovine sperm treatment method, firstly, the sperm is treated by using a weakly alkaline solution, and therefore, the tail part of the sperm is shortened by 1 / 3-1 / 2; and then, the sperm of which the tail part is shortened by 1 / 3-1 / 2 is selected and is injected into the oocytes. After the bovine sperm treatment method disclosed by the invention is adopted for carrying out the treatment, only the tail part of the sperm is shortened by 1 / 3-1 / 2, the sperm still retains the centrosome, the integrity of the sperm is structurally retained, the method is more similar to the change of sperm capacitation in a natural fertilization process, the embryonic development potential is improved, and the development rate of microinjection embryos is improved. In addition, the tail part of the sperm treated by the bovine sperm treatment method is shortened by 1 / 3-1 / 2, and shortening of the tail part can lower the difficulty of microinjection, improve the operation speed of injection and improve the efficiency of intracytoplasmic sperm injection.

A sperm injection tube for artificial insemination of swine has a tube and a plug mounted on an end of the tube. The plug has a plug body, a guiding portion, and a blocking portion. The guiding portion is formed around the plug body and has multiple guiding fins near the injection hole. The blocking portion is formed around the plug body and has at least one blocking fin near the connecting hole. Each guiding fin is umbrella-shaped and flexible. When inserting the sperm injection tube into the uterus, the guiding fins are concentrated toward the plug body to led the plug to easily pass through the cervix. The blocking portion block the cervix to prevent the semen flowing out and to keep the tube from being pushed out to raise the conception rate of the sow.

The invention discloses a microsperm cryopreservation method, aiming to provide a microsperm with small volume, closed space, easy operation and high sperm activity (including a single sperm, such as testicular puncture sperm, epididymal puncture sperm, severe The cryopreservation carrier and cryopreservation method of the sperm of patients with oligospermia and asthenospermia, etc.) When the sperm is recovered, take out the cryopreservation tube from the liquid nitrogen tank, take out the cryopreservation carrier from the cryopreservation tube with a straw, and quickly add it to the sperm culture dish Medium (37°C), under an inverted microscope (100-200 times magnification), carefully search for sperm in the frozen carrier, use the intrafollicular single sperm injection needle to grab the sperm and transfer them to the sperm culture medium of the sperm culture dish for subsequent sperm ICSI of immobilized and matured eggs.

The invention discloses a poultry insemination device, which comprises an extruding head, a pressure storage section, a semen storage section, a jetting section, a working body, a pressure suction body and a scale line. The feature is that the suction body is installed on the top of the working body, and the suction body is a cone-shaped body with a large upper part and a smaller lower part. The upper part of the working body is the pressure storage section, the middle part is the semen storage section, and the lower part is the injection section. The bottom port of the pressure head is made in cooperation, the top port of the pressure storage section is inserted into the bottom port of the extrusion head to seal, and the outer diameter of the injection section is marked with a scale mark. The advantage is that it can not only collect and inoculate the breeding cock conveniently and quickly, but also accurately calculate the ejaculation volume of the breeding cock, so as to achieve the purpose of convenient and quick insemination, and ensure the smooth development of artificial insemination of the breeding chicken. It has a simple structure and is easy to manufacture. Easy to operate, accurate, clean and hygienic.

The invention relates to the technical field of livestock breeding, in particular to an equine animal artificial insemination and semen injection device which comprises a tubular rack, a telescopic rod array, a rubber film, an inner transmission wire harness, an outer transmission wire harness, a wire harness connection synchronizer and a semen injection tube. Wherein the tubular rack is a herringbone rack and is divided into an inner branch section, an outer branch section and a connecting section, the forward extension length of the inner branch section is larger than that of the outer branch section, the two telescopic rod arrays are arranged at the front ends of the inner branch section and the outer branch section respectively, and the two rubber films wrap the two telescopic rod arrays respectively; by means of conformal display of the mutually-linked array telescopic rods, precise positioning of sperm injection operation is achieved, the influence of temperature rise of electric equipment of an endoscope on sperm activity is eliminated, and the equipment cost is remarkably reduced.

A method of enhancing embryo implantation in a subject is disclosed which comprises administering to the uterine cavity of the subject a formulation comprising copper and / or zinc in an amount effective to stimulate endometrial production of leukaemia inhibitory factor (LIF) and / or vascular endothelial growth factor (VEGF). Alternatively, a device may be inserted into the uterine cavity of the subject, wherein the device comprises copper and / or zinc, for a period of time that is effective to stimulate endometrial production of LIF and / or VEGF. The method is suitable for use with women undergoing treatment by any of the assisted reproductive technologies, such as those involving the transfer of embryos such as in vitro fertilisation (IVF) and variants including IVF-ICSI (intracytoplasmic sperm injection) and in vitro maturation (IVM) treatments, as well as intrauterine-insemination (IUI) therapy. However, the method is also applicable for women wanting to improve their prospects of pregnancy through natural conception.

The invention discloses a mouse microorganism purifying method utilizing an egg cell cytoplasm sperm injection method. After an adult mouse is killed, the male reproductive organ is taken out from the adult mouse under the aseptic condition and is placed in the 75% ethyl alcohol to be immersed and disinfected, and then the sperms are obtained. After the freezing, reviving and docking procedures are carried out on the sperms, the heads of the sperms are injected into the cytoplasm of ova one by one to form germ cells, and after being cultivated, the germ cells are used for embryo transplantation. The egg cell cytoplasm sperm injection method is applied to the mouse microorganism purification, the method for processing the sperms in the process of injecting is improved, a new method is provided for the microorganism purification of an experimental animal, and meanwhile, a new thought is provided for germplasm resource storage and other studies.

The invention belongs to the technical field of medicines and relates to a sperm freezing method based on ICSI micromanipulation. The method comprises the following steps: (1) preparing two ICSI dishes, introducing sperm samples into one ICSI dish, and introducing one or multiple drops of sperm freezing protecting liquid into the other ICSI dish; (2) extracting motile sperms from the ICSI dish containing the sperm samples by virtue of a single sperm injection needle, and introducing the motile sperms into liquid drops in the sperm freezing protecting liquid in the other ICSI dish; and (3) putting the ICSI dish containing the liquid drops of the sperm freezing protecting liquid into liquid nitrogen, freezing, and carrying out preservation. The method has the beneficial effects that a carrier is saved, the intermediate links of a small amount of sperms in the freezing and unfreezing processes are remarkably reduced, the activity ratio of the unfrozen sperms is high, the loss rate is low,and the utilization rate of the sperms is high.

The invention discloses a follicle puncture detection method based on near-infrared vision. The follicle puncture detection method comprises the following steps: firstly, grabbing a follicle punctureoperation surface by a near-infrared camera, wherein the operation surface comprises a follicle to be injected and an injection tube carrying sperms, and realizing injection tube calibration accordingto the fact that the injection tube carrying the sperms is a unique moving object in the operation surface; and determining an intersection point with follicular zona pellucida along the moving direction of the calibrated injection tube, determining an expected position of a position identifier according to the intersection point, and finally completing detection according to whether the positionidentifier appears at the expected position. Position identification is set in a self-adaptive mode according to the light and shade difference of gray level images of the zona pellucida, substancesin the zona pellucida, the sperms in the injection needle and seminal plasma under near-infrared light, follicle puncture is judged to be successful when the position identification appears in the visual field, and automatic control injection is achieved. In an in-vitro fertilization and embryo transplantation combined technology, the puncture effect can be effectively stabilized and the success rate of one-time puncture injection can be improved by means of injecting single sperms in egg pulp.

A method for preserving spermatozoa (sperm) by air-drying. A semen sample is received and the sperm are separated from contaminants in the semen sample. The sperm may be separated from the semen sample by layering the semen sample onto a two-layer discontinuous percoll layer followed by centrifugation or by centrifuging the semen sample. The sperm concentrate is spread onto a sterile plate and allowed to air dry. The air-dried sperm may be stored at 0° C. to 8° C. The air-dried sperm may be recovered by adding a warm physiological solution to the air-dried sperm. The recovered sperm may be used for in vitro fertilisation or for intracystoplasmic sperm injection (ICSI).

The invention relates to a La-1-like peptide agent that increases sperm motility. It is non-toxic and it may be used to treat male infertility in humans, and also to increase fertility in animals. It may be used in artificial reproductive techniques such as in vitro fertilisation (IVF), including intra-cytoplasmic sperm injection (ICSI). The La-1 like peptide agent may also be used in artificial insemination techniques such as intra-cytoplasmic uterine injection (IUI).