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Composition for promoting sperm activation, sperm activating solution and preparation method for sperm activating solution

A composition and sperm technology, applied in the field of assisted reproduction, can solve the problems of large differences in the application effects of exogenous substances, short-term stimulation of sperm, and failure to improve the structure and function of sperm, so as to avoid the oxidative stress of sperm and repair Effects on sperm structure, increased fertilization rate and blastocyst formation rate

Active Publication Date: 2020-05-01
成都艾伟孚生物科技有限公司
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Problems solved by technology

However, the application effects of different exogenous substances are quite different, and they can only produce short-term stimulation to sperm, so they cannot improve the structure and function of sperm

Method used

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  • Composition for promoting sperm activation, sperm activating solution and preparation method for sperm activating solution
  • Composition for promoting sperm activation, sperm activating solution and preparation method for sperm activating solution
  • Composition for promoting sperm activation, sperm activating solution and preparation method for sperm activating solution

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preparation example Construction

[0045] 2. The preparation method of sperm activation liquid in embodiment 1~5, comprises the following steps:

[0046] S1. prepare solution I, comprise the following steps:

[0047] 1) Make the required utensils for preparation go through dry heat at 250°C for 30 minutes to remove the heat source;

[0048] 2) Under the condition of grade 100, weigh the ATP sodium salt, heparin, phosphatidylserine, linolenic acid, sodium chloride, potassium chloride, magnesium sulfate heptahydrate, potassium dihydrogen phosphate, calcium chloride dihydrate by proportioning , sodium fluoride, glucose, sodium pyruvate, sodium lactate, amino acid and bactericide, and added to water and mixed uniformly to obtain solution I;

[0049] S2. Weigh the human serum albumin, vinculin, allene oxide synthase, acetyl-CoA acetyltransferase 2, coenzyme Q10 and recombinant heat shock protein HSPA8 according to the ratio, and add them to the solution I Mix well to obtain solution II;

[0050] S3. After filteri...

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Abstract

The invention relates to the field of assisted reproduction, and discloses a composition for promoting sperm activation and a sperm activation solution. The sperm activation solution comprises a basicculture solution and a composition, wherein the composition comprises 2-10mg / L of ATP sodium salt, 5-15 U / mL of heparin, 5-10 [mu]g / mL of spot adhesion protein, 1-2.5[mu]g / mL of allene oxide synthase, 4-4.5 [mu]g / mL of acetyl coenzyme A acetyltransferase 2, 0.9-1.1 mmol / L of phosphatidylserine, 5.5-10.5 [mu]g / mL of coenzyme Q10, 3.5-6.5 ng / mL of linolenic acid and 0.45-0.5 [mu]g / mL of recombinantheat shock protein HSPA8. The invention also discloses a preparation method for the sperm activation solution. According to the sperm activation solution, the time of sperm injection and ovum in-vitro operation can be greatly shortened; the fertilization rate and the blastocyst formation rate are increased; the pregnancy rate is directly or indirectly increased; meanwhile, the sperm activation solution is effectively suitable for activation and optimization of sperms in an oocyte intracytoplasmic single sperm microinjection technology (ICSI) and an in-vitro fertilization (IVF) process in an in-vitro fertilization assisted reproduction technology.

Description

technical field [0001] The invention relates to the field of assisted reproduction, in particular to a composition for promoting sperm activation, a sperm activation liquid and a preparation method thereof. Background technique [0002] In vitro fertilization (in vitro fertilization, IVF), also known as test-tube baby, is a method of assisted reproductive therapy. Specifically, it refers to taking out eggs and sperm, performing in vitro fertilization, culturing and forming embryos, and then transferring the embryos back to the mother's uterus The inner pregnancy develops into a fetus. [0003] About 35-40% of infertile patients in assisted reproductive treatment cycles come from male factors, and the second-generation IVF is the main technology to solve male factor in vitro fertilization. The second-generation test-tube baby, also known as intracytoplasmic sperm injection (ICSI), in layman's terms, is to pick out a sperm with the strongest motility and the best quality unde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076
CPCC12N5/061C12N2500/12C12N2501/91C12N2501/998C12N2501/71C12N2501/72C12N2500/32C12N2500/36C12N2501/999C12N2500/16C12N2500/14C12N2500/30C12N2500/34
Inventor 余裕炉米乐叶林刘洪君严飞戴甄
Owner 成都艾伟孚生物科技有限公司
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