417 results about "Sodium pyruvate" patented technology

The invention provides methods of removing contaminants from a mixture of a desired product and contaminants by pH adjustments and molecular weight cut-offs. The contaminants include phosphate groups, magnesium sulfate, sodium pyruvate and tetrasodium pyrophosphate groups. The desired product includes nucleotide sugars, glycolipids, LnNT, sialyl lactose, and salts.

The invention discloses a serum-free medium for culturing placenta mesenchymal stem cells. The serum-free medium takes a DMEM (Dulbecco Modified Eagle Medium) culture solution as a basis and also contains a fibroblast growth factor receptor 2, growth hormone, insulin, transferrin, glutathione, BMP-4, L-glutamine, sodium pyruvate, non-essential amino acids and beta-mercaptoethanol. According to various serum-free media provided by the invention, growth and proliferation of the placenta mesenchymal stem cells in a serum-free medium system can be effectively promoted, the placenta mesenchymal stem cells have higher growth and proliferation rate in the serum-free medium system compared with a serum cell culture medium, the characteristics of the stem cells are preserved, the serum-free medium has multiple differential potentials, and the stem cells can be directionally induced into fat cells and osteoblasts.

The invention provides a culture fluid exclusively used in cow in-vitro fertilization embryos. The culture fluid contains 109.0mM-110mM of NaCl, 2.9mM-3.1mM of KCl, 26.0mM-26.5mM of NaHCO3, 0.5mM-1.0mM of MgCl2.6H2O, 1.0mM-1.3mM of KH2PO3, 0.4mM of sodium pyruvate, 1.5 mM of glucose, 5mM of galacturonic calcium, 10v / v% fetal bovine serum, 1mM of L- glutamine, 2v / v% essential amino-acid, 1v / v% nonessential amino acid and 1mM-10mM of glutathione. The cow in-vitro fertilization embryos are placed into the culture fluid to carry out in-vitro cultivation, and results are shown to be obviously superior to those of a control group which is not added with GSH (glutathione), and therefore, developmental rate of blastula and embryo quality are improved, cost for producing embryos in vitro is lowered, experimental basis is provided for applying a cow IVF (in vitro fertilization) technology to practice, and a genetic breeding process can be greatly accelerated.

The invention provides a mycoplasma hyopneumoniae culture medium and a preparation method thereof, belonging to the technical field of veterinary biology. The mycoplasma hyopneumoniae liquid culture medium comprises the components as follows: brain heart infusion, lactalbumin hydrolysate, PPLO (pleuropneumonia-like organism) broth, yeast extract powder, proteose peptone, sodium thiosulfate, Hank's liquid, sodium pyruvate, 0.1% phenol red solution, penicillin and deionized water. The preparation method comprises the following steps of: adding health horse serum before using, and adding agar into the liquid culture medium to obtain a solid culture medium of mycoplasma hyopneumoniae. The viable bacteria titer of the mycoplasma hyopneumoniae culture medium can reach 1*109CCU / ml-1*1010CCU / ml; the viable bacteria titer and the separation sensibility are far higher than those of the existing culture medium, and the mycoplasma hyopneumoniae is fast in growth speed and high in the separation sensibility; and the preparation method of the culture medium is simple in technology, strong in operability, and suitable for industrial large-scale production.

The invention discloses a complex enrichment medium used for salmonella, listeria monocytogenes and staphylococcus aureus and a method for preparing the same. The complex enrichment medium comprises the following components in weight portion: 15 to 19 portions of tryptone, 2 to 4 portions of peptone, 2 to 3 portions of sodium dihydrogen phosphate, 2 to 3 portions of glucose, 0.5 to 1.5 portions of aesculin, 1, 000 portions of distilled water, 1 to 2.5 portions of sodium pyruvate, 3 to 10.0 portions of mannitol, 1.0 to 25.0 portions of sodium chloride, 1 to 2.5 portions of lithium chloride, 0.0001 to 0.0002 portion of potassium tellurite, and 0.005-0.015 portion of nalidixic acid, wherein the pH value is between 7.1 and 7.5. The complex enrichment medium can inhibit the growth of other pathogenic microorganisms while performing enrichment on three target pathogens, thus the complex enrichment medium can be directly applied to the isolation culture and the biological assay test of target bacteria, and can also be directly applied to a detection technology of a plurality of pathogens based on one detection platform for multiple PCR and the like to make a diagnostic report.

The invention relates to a serum-free adipose tissue-derived mesenchymal stem cell culture medium, which consists of a basic culture medium and added ingredients, wherein the basic culture medium is DMEM-LG, and the added ingredients and the content of each added ingredient are shown as follows: 5 to 20ng / mL of alkaline fiberblast growth factors, 5 to 20ng / mL of epidermal growth factors, 100U / mL of penicillin, 100 micrograms / mL of streptomycin, 50 to 200 micrograms / mL of heparin, 2 to 8mM of L-glutamine, 100 to 300 microM of 2-mercaptoethanol, 500 to 2000U / mL of leukaemia inhibitory factors and 0.5 to 2mM of sodium pyruvate. The serum-free adipose tissue-derived mesenchymal stem cell culture medium does not contain the serum, so that the inter-batch difference and the influence of the serum component on the cell culture can be avoided; the exogenous pollution of the serum and the toxicity of the serum on the cells can be avoided; and the ingredients are clear, so that the research of the psychological regulation mechanism of the cells can be facilitated.

The invention relates to a method for inducing differentiation from mesenchymal stem cells to cartilage cells and application of the mesenchymal stem cells in osteoarthritis, and provides a method for preparing umbilical cord mesenchymal stem cells differentiated to the cartilage cells, mesenchymal stem cells differentiated to the cartilage cells and application of the prepared drug prepared by the mesenchymal stem cells in treatment of the osteoarthritis. The method comprises the following steps of: inducing and culturing the mesenchymal stem cell in a 15ml taper-bottom centrifuge tube by using a globule method; and slightly shaking the centrifuge tube for one time every 24 hours to separate cell sphere from the bottom of the tube, wherein the differentiation inducing culture medium is prepared by adding 100mu g / ml of sodium pyruvate, 10ng / ml TGFbeta3, 100nM of dexamethasone, 25mu g / ml of vitamin C, 40mu g / ml of proline and 1*ITS and 1 of premix into a high-glucose dulbecco modified eagle medium (DMEM). A non-bracket cartilage built by the method can be used for repairing damaged articular cartilages.

The invention relates to a culture medium for simultaneous composite enrichment of five kinds of food-borne pathogenic bacteria, namely Salmonella, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Shigella, and a preparation method for the culture medium. Food-borne pathogenic bacteria are a significant reason to cause food positioning, so the rapid and accurate detection of the food-borne pathogenic bacteria has an important practical significance for preventing and controlling food safety incidents. The culture medium is characterized by comprising the following components: 10.0 g of peptone, 10.0 g of sodium chloride, 9.0 g of disodium hydrogen phosphate, 1.5 g of monopotassium phosphate, 0.1 g of cholate, 0.1 mg of potassium tellurite, 1.0 g of lithium chloride, 3.0 g of glucose, 2.0 g of mannitol, 2.5 g of sodium pyruvate, 1.0 g of aesculin and 1,000 mL of distilled water, wherein the pH value is 7.1 to 7.5. The culture medium can simultaneously enrich five kinds of target pathogenic bacteria, can be used for separation and identification of target bacteria, can also be used for the molecular detection of multiple pathogenic bacteria on the same detection platform, provides technical support for a method for rapidly detecting five kinds of pathogenic bacteria in food, and meets the requirement of simultaneous detection of five kinds of food-borne pathogenic bacteria.

The invention discloses a chromogenic medium of a coliform group and a quick detection card thereof. The medium contains peptone, yeast extract, lactose, common salt, sodium pyruvate, SDS, sodium dihydrogen phosphate, a specific enzyme chromogenic substrate and IPTG. The quick detection card consists of, in a bottom-up order: a negative film, a water absorption and moisture retention layer, a medium layer and a covering membrane, with the above medium adsorbed on the medium layer. The chromogenic medium of a coliform group and the detection card that are provided in the invention can detect coliform groups in drinking water, surface water or foodstuff, with short detection time and high efficiency. The chromogenic medium and the detection card of the invention can conduct qualitative detection to coliform groups, and simultaneously can carry out quantitative analysis. And a positive result has obvious color development and is convenient for observation, odds for emergence of false positive and false negative can be reduced, and the detection result is accurate.

The invention relates to a complex enrichment medium used for salmonella, vibrio parahaemolyticus and comma bacillus and a method for preparing the same. The formulation components of the complex enrichment medium in weight portion are as follows: 0.5 to 1.5 portions of buffer peptone water, 1000 portions of distilled water, 1.0 to 5.0 portions of glucose, 1.0 to 5.0 portions of mannitol, 0.03 to 0.06 portion sodium pyruvate, 0.5 to 2.5 portions of anhydrous sodium sulfite, 1.0 to 10.0 portions of sodium citrate, 5.0 to 25.0 portions of sodium chloride, 1 to 5 portions of cholate, and 0.0005 to 0.0025 portion of potassium tellurite. The method comprises the following steps: firstly, adding the buffer peptone water and the like into the distilled water, and sterilizing the mixture after heating and melting; and secondly, cooling the mixture to 50 DEG C, and then adding the potassium tellurite into the mixture to be mixed evenly. The complex enrichment medium can integrate two steps of primary enrichment and selective enrichment of bacterial detection to shorten the enrichment time to 24 hours, can perform enrichment on three target pathogens at the same time, and can inhibit the growth of other microorganisms.

The invention discloses a building method of fish head and kidney tissue derived cell lines and the obtained cell lines. The cell lines are obtained by performing a primary culture and a subculture for the treated fish heads and kidney tissues, wherein an employed cell culture medium is prepared by adding fetal calf serum, sodium pyruvate, 2-mercaptoethanol and a human basic fibroblast growth factor into a basic cell culture medium.

The invention discloses a method for continuously separating sialic acid, wherein sialic acid is prepared through microbial fermentation by taking N-acetyl glucosamine and sodium pyruvate as substrates; fermentation liquor is processed through filtration and ultrafiltration so as to obtain sialic acid clear liquid; sialic acid clear liquid is pumped in a continuous ion exchange system provided with OH-type anion exchange resin for adsorption; deionized water is adopted for leaching; a sodium chloride solution is adopted for elution; a sodium hydroxide solution is adopted for resin regeneration; eluent containing sialic acid is collected, is leached through an ethanol water solution, and then is added with ethyl acetate for dilution crystallization; and crystals are dried under reduced pressure, so that pure sialic acid is obtained. The separation and purification method is simple, convenient and feasible, achieves good effect and low running cost, and can implement the separation from kilogram class to ton class according to the separation amount; and in addition, products that are obtained by adopting the method gain excellent results on yield coefficient and product quality, and the product quality is ensured..

The invention discloses an inducer and a method for inducing differentiation of adipose-derived stem cells to cartilage cells, belonging to the technical field of cell biology. According to the technical scheme, the inducer comprises TGF-Beta 3, FGF-18, dexamethasone, insulin, selenium, transferrin, linoleic acid, BSA, sodium pyruvate solution, L-ascorbic acid-2-phosphate and proline. The inducer and the method have the advantages that the synthesis and metabolism of the cartilage cells are rapidly promoted, the generation of cartilage is promoted, the expression of cartilage extracellular matrix II type collagen and glycosaminoglycan is remarkably improved, and the time required for in-vitro induction culture is reduced; and the inducer and the method are widely suitable for various culture systems, especially a pellet system and a three-dimensional cell scaffold culture system.

The present invention relates to a nasal spray containing sodium pyruvate and a preparation method thereof, belonging to the field of medical technology, being characterized in that: the nasal spray containing sodium pyruvate comprises the following components: sodium pyruvate, pyruvic acid, an isoosmotic adjusting agent, a preservative and water. Because the sodium pyruvate is capable of alleviating nasal obstruction and inflammation caused by rhinitis, a medical fluid provided in the invention can be made into a nasal spray medical fluid which is capable of entering nasal cavities by adopting a portable spraying device. The nasal spray medical fluid is particularly suitable for cleaning and removing harmful pollutants in nasal passages, paranasal sinuses and mucosal cilia and is capable of being used in nursing after a nasal cavity operation, relieving rhinostegnosis and reducing respiratory tract irritation and risks of infection. By adding the pyruvic acid to form a buffer solution, tingling caused by using the flushing fluid to wash the cavities can be reduced, and inflammation is alleviated. Allergic symptoms can be prevented or gradually alleviated for the people of special professional who often use the product provided in the invention to wash and care nasal cavities.

The invention relates to an amniotic membrane long-term preserving fluid which is characterized in that 1000ml of preserving fluid contains 10g-200g of chondroitin sulfate and low-molecular-weight dextranum, 10g-50g of hydroxyethyl starch, 5ml-20ml of amino acid, 5ml-20ml of tobramycin, 10ml-50ml of HEPES, 5ml-30ml of ascorbic acid, 5ml-20ml of sodium pyruvate, and 860ml-970ml of culture solution. A preparation method of the amniotic membrane long-term preserving fluid comprises the following steps: adding the chondroitin sulfate, the low-molecular-weight dextranum, the amino acid, the tobramycin, the hydroxyethyl starch, the ascorbic acid and the sodium pyruvate into the culture solution and then uniformly mixing the mixture; using 1%-5% of HEPES to adjust pH value till the pH value is 7.2-7.4; using an osmotic pressure buffering agent to adjust the osmotic pressure till the osmotic pressure is 350-380mOsm / L; and performing membrane filtration and degerming, thereby acquiring the amniotic membrane long-term preserving fluid.

A serum-free cryoprotectant includes: 8-15 v / v% of DMSO, 85-92 v / v% of a DMEM basic culture medium, and a nutritional additive. The nutritional additive includes: fibroblast growth factors, insulin, growth hormone, transferrin, bone morphogenetic protein 4, glutamine, sodium pyruvate, beta-mercaptoethanol, human epidermal growth factor, sodium selenite, and various amino acids and vitamins. The cryoprotectant is free of animal sourced serum and avoids pollution and risk of allergen, and has better clinical safety. The serum-free cryoprotectant is suitable for cryopreservation of human placenta sourced, umbilical cord sourced and cord blood sourced mesenchymal stem cells; compared with common serum cryoprotectants, perinatal mesenchymal stem cells preserved in the cryoprotectant have high cell survival rate after resuscitation, have excellent adherence growth status and maintain biological characters well.

The invention discloses a composite enrichment medium for salmonella, staphylococcus aureus, escherichia coli, listeria monocytogenes and vibrio parahaemolyticus and a preparation method for the composite enrichment medium. The composite enrichment medium comprises the following components in parts by weight: 5-10 parts of ox brain extract powder, 5-15 parts of ox heart extract powder, 5-15 parts of peptones, 2.5-7.5 parts of sodium chloride, 1-3 parts of glucose, 1.5-3.5 parts of disodium hydrogen phosphate, 1-3 parts of mannitol, 1-3 parts of sodium pyruvate, 1-3 parts of glycine, 1-3 parts of aesculin, and 1000 parts of distilled water, and a pH value of the composite enrichment medium is 7.2-7.5. A preparation method for the composite enrichment medium comprises the following steps: adding the components into distilled water according to the formula, stirring and dissolving the components, regulating the pH value of the mixture, and sterilizing the mixture at high pressure. The five bacteria are main pathogenic bacteria which are needed to be detected according to the national food hygienic standard. The composite enrichment medium can be used for realizing rapid proliferation of the five pathogenic bacteria. After carrying out bacteria enrichment for 16 hours, the concentration of the thallus can be increased to 10<6>CFU / mL-10<9>CFU / mL from 1CFU / mL, so that the thallus can be directly used for carrying out high flux detection on multiple PCRs (polymerase chain reactions), gene chips and micro-fluidic chips, and thus, screening of the five pathogenic bacteria is completed.

The invention discloses a human mesenchymal stem cell cartilage-formation induced differentiation culture medium. The human mesenchymal stem cell cartilage-formation induced differentiation culture medium is characterized by being prepared from the following components: an alpha-MEM / HG-DMEM culture medium, fetal bovine serum (FBS) with the volume percent of 5 to 50 percent, sodium pyruvate with the concentration of 50 to 200mg / ml, ascorbic acid with the concentration of 20 to 200mu g / ml, proline with the concentration of 10 to 100mu g / ml, dexamethasone with the volume of 50 to 500nM, ITS withthe volume percent of 0.5 to 20 percent, TGF (Transforming Growth Factor)-beta with the concentration of 0.1 to 20ng / ml and an insulin growth factor-2 with the volume of 0.5 to 50nM. The human mesenchymal stem cell cartilage-formation induced differentiation culture medium is prepared through the following steps: disinfecting a culture dish; adding a source culture medium; mixing the components; adding FBS, a sodium pyruvate mother solution, an ascorbic acid mother solution, a proline mother solution, a dexamethasone mother solution, an ITS mother solution, a TGF-beta3 mother solution and an insulin growth factor-2 mother solution according to the concentration, and uniformly mixing; filtering and sterilizing; filtering and sterilizing a mixed culture solution by utilizing a 0.22mu m filtering membrane. The human mesenchymal stem cell cartilage-formation induced differentiation culture medium has a rapid induction speed, and has a better induced differentiation effect on differentiation raw materials with different sources, such as human bone mesenchymal stem cells, human umbilical cord mesenchymal stem cells and human adipose mesenchymal stem cells; a preparation method of the culture medium is simple, the culture medium is convenient to use and a process is stable.

The invention relates to a method for improving the cultivation efficiency of bovine in vitro fertilization embryos. In particular, the invention relates to bovine in vitro fertilization embryo cultivation liquid. The cultivation liquid contains NaCl, KCl, NaHCO3, MgCl2, KH2PO3, sodium pyruvate, glucose, calcium galactonate, fetal calf serum, L-glutamine, necessary amino acid, unnecessary amino acid, glutathione and water. The invention also relates to a method for improving the cultivation efficiency of the bovine in vitro fertilization embryos. The method comprises the following steps: putting the bovine in vitro fertilization embryos into the bovine in the vitro fertilization embryo cultivation liquid, and performing embryo in vitro cultivation under the conditions of 38.5 DEG C, 0.5 percent CO2 and 100 percent humidity. The method provided by the invention and the used cultivation liquid present excellent technical effects as stated in the specification.

The invention relates to a serum free culture medium for in vitro maturation of bovine oocyte, belonging to the field of cytobiology, in particular to a serum free medium IVMBM-I for in vitro maturation of bovine oocyte, comprising a TCM199 medium, hormone for oocyte maturation and antibacterial agent, which is characterized in that: Sodium pyruvate, Taurine, Insulin, Selenium, BSA, HEPES, TGF-a, L-Glutamine and EGF are added, and the serum is not contained. The serum free culture medium for in vitro maturation of bovine oocyte has the advantages of avoiding the adverse effect of some components in the serum on the oocyte, exact quantification and good repeatability because the conventional serum is substituted by the added components, and that the function of the serum in the maturation process of the oocyte can be completely substituted by the added components is proved by the experiments of in vitro maturation, in vitro fertilization and in vitro development.

The invention relates to a method for establishing and identifying a cynoglossus semilaevis testis cell line, which comprises the following steps: adopting a MEM (Minimum Essential Medium), adding 20 percent of fetal calf serum, 0.1 percent of B-mercaptoethanol, 2 ng / ml of human alkaline fibroblastic growth factors, 1 ng / ml of leukemia inhibiting factors, 1 nmol of sodium pyrurate and 1 nmol of glutamine and preparing into a complete medium; inoculating a tissue block into a culture bottle, overturning and inversely placing the bottle to culture for 3 h and positively placing the culture bottle after the tissue block is attached onto a wall, so that the tissue block is soaked into the culture medium for culture. A trypsin digestion method is adopted for subculturing cells. One cynoglossus semilaevis testis cell line is established by adopting the above method and is subcultured to fiftieth generations. Meanwhile, the invention also provides a method for identifying a chromosome of the established cell line and the level of a molecular marker and a method for identifying the sensitivity of the cell line to related viruses. The method for establishing the cynoglossus semilaevis testis cell line can be used for culturing other fish gland cells and establishing the cell line, thereby having wider promotion and application prospect.

The invention belongs to the technical field of pig genetic breeding, and specifically relates to a culture solution capable of improving in vitro maturation rate of pig oocytes and application thereof. The culture solution capable of improving the in vitro maturation rate of pig oocytes is prepared from the following raw materials with the following concentrations: 9.5 g / L of TCM-199, 2.2 mg / mL of NaHCO3, 3.05 mmol / L of D-glucose, 0.91 mmol / L of Sodium Pyruvate, 0.5 g / L of Streptomycin sulfate, 0.05% of PVA, 0.57 mmol / L of Cysteine and 0.075 g / L of Penicillin G. The 0.05% of PVA and 5% of a follicular fluid are added on the basis of pig oocyte in vitro maturation culture solution widely used in our country, so that the in vitro maturation rate of the pig oocytes can be improved to more than 90%, and matured oocytes with higher quality and more quantity can be provided for porcine somatic cell nuclear transfer technology.

The invention discloses an osteochondral graft preservation liquid and a preparation method thereof. The osteochondral graft preservation liquid comprises the following raw materials: 80-125mmol of glucose, 0.1-2.0mmol of amino acid, 0.2-2.0mmol of an antioxidant, 5-60nmol of a basic fibroblast growth factor, 1-120mmol of inorganic salt, 1-9mmol of vitamin, 1-2mmol of sodium pyruvate, 50-70U of penicillin and the balance of deionized water. The method comprises the following steps: preparing a solution, adjusting the pH to be 7.30-7.50, filtering and degerming; cleaning fresh allogeneic osteochondral graft with a sterile saline solution for 3-5 times, and putting into the prepared osteochondral graft preservation liquid to soak; and storing at 4 DEG C. The osteochondral graft preservation liquid has the efficacies of resisting cartilage cells senescence in preservation, inhibiting cartilage cells apoptosis, keeping good biological activity of cartilage tissues, maintaining the survival rate of cartilage cells, prolonging the storage time and improving the storage effect of the osteochondral graft.

The invention relates to a culture solution for bovine IVF and a method for improving bovine IVF. The method for bovine IVF comprises the following steps: washing a mature cumulus-oocyte complex in afertilization culture solution, transferring the mature cumulus-oocyte complex into the fertilization culture solution, and putting the fertilization culture solution into an incubator for later use;taking a frozen seminal tubule from liquid nitrogen, performing unfreezing in a water bath at 37 DEG C, injecting seminal fluid into a centrifuge tube containing a seminal fluid preparation culture solution, and discarding a supernatant after centrifugation; adding the seminal fluid preparation culture solution into the centrifuge tube, resuspending sperm precipitate, and taking a proper sperm suspension for sperm counting; and adding the sperm suspension with the calculated volume into fertilization culture liquid drops containing oocytes, placing a culture tray into the incubator, and carrying out sperm-ovum co-incubation to complete IVF operation for embryo in-vitro culture. The fertilization culture solution and the seminal fluid preparation culture solution contain sodium pyruvate, heparin and other components. The method can significantly improve the efficiency of bovine IVF.

The invention discloses a culture solution for an in vitro efficient amplification of animal cells and an application of the culture solution. The culture solution consists of basal culture mediums, namely DMEM / F12, bFGF, EGF, sodium pyruvate, glutamine, sodium hydrogen carbonate and fetal calf serum. The culture solution disclosed by the invention has comparatively low content of animal serum, and is comparatively high in safety, simple and definite in formula components; the culture solution can avoid a possibility of different properties of the cultured cells due to a difference between culture medium batches, and is applicable to industrial production of cell products. Animal adherent cells cultured by the culture solution disclosed by the invention are comparatively strong in in-vitro amplification capacity and well maintain biological characteristics; and the culture solution is not only applicable to culture of adherent mesenchymal stem cells, but also suitable for culture of other fibroblast lines and other cell lines.

The invention relates to a method for bovine in-vitro fertilized embryo culture and a used culture solution. Particularly, on one hand, the invention relates to a method for culturing a bovine in-vitro fertilized embryo. The method comprises the following steps of putting the bovine in-vitro fertilized embryo into a bovine in-vitro fertilized embryo culture solution and carrying out embryo in-vitro culture under the conditions that the temperature is 38.5 DEG C, the content of CO2 is 0.5% and the humidity is 100%. The invention further relates to a bovine in-vitro fertilized embryo culture solution. The culture solution comprises NaCl, KCl, NaHCO3, MgCl2, KH2PO3, sodium pyruvate, glucose, calcium galactonate, fetal calf serum, L-glutamine, an essential amino-acid, a nonessential amino acid, glutathione and water. The method and the used culture solution have excellent technical effects in the specification, for example, the method has a higher blastocyst hatching rate when the bovine in-vitro fertilized embryo is cultured.