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Method for continuously separating sialic acid

A technology of sialic acid and continuous ions, which is applied in the direction of chemical instruments and methods, sugar compounds with non-glycosyl groups, sugar derivatives, etc. It can solve the problems of unsuitable industrial production, poor separation and purification effect, and only intermittent operation, etc. problems, to achieve the effect of low operating cost, simple and easy separation and purification method, and increased concentration

Active Publication Date: 2012-07-04
NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The main purpose of the present invention is to overcome the disadvantages of high production cost, cumbersome operation, poor separation and purification effect, difficult scale, only intermittent operation and unsuitable for industrialized production in the process of using fixed bed ion exchange technology to separate sialic acid in the prior art

Method used

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  • Method for continuously separating sialic acid
  • Method for continuously separating sialic acid
  • Method for continuously separating sialic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] After the fermentation broth is filtered, most of the protein is removed through an ultrafiltration membrane with a molecular weight cut-off of 10,000 Daltons, and the supernatant is diluted to a concentration of sialic acid of about 5g / L, and then pumped into a continuous ion exchange machine filled with anion exchange resin. system. A continuous ion exchange system consisting of 12 anion exchange columns is adopted, with 3 columns in the adsorption area, 3 columns in the impurity washing area, 4 columns in the elution area, and 2 columns in the regeneration area. Each zone can be used in series to increase the concentration of the target product effluent. Each ion exchange column was loaded with 100 g of resin. The adsorption flow rate is 3ml / min, and the adsorption capacity is 0.341g / g wet resin; the washing solution is deionized water, and the flow rate is 3ml / min, and the washing is done until the effluent is free of impurities (use HPLC to determine the first pla...

Embodiment 2

[0045] After the fermentation broth is filtered, most of the protein is removed through an ultrafiltration membrane with a molecular weight cut-off of 30,000 Daltons, the supernatant is diluted to a concentration of sialic acid of about 10 g / L, and pumped into a continuous ion exchange machine filled with anion exchange resin. system. A continuous ion exchange system consisting of 14 anion exchange columns is adopted, 4 in the adsorption area, 3 in the impurity washing area, 4 in the elution area, and 3 in the regeneration area. Each zone can be connected in series to increase the concentration of the target product effluent. Each ion exchange column was packed with 400g resin. The adsorption flow rate is 15ml / min, and the adsorption capacity is 0.244g / g wet resin; the washing solution is deionized water, and the washing flow rate is 25ml / min, and the washing is performed until the effluent is free of impurities (use HPLC to determine the area of ​​the washing area). The fir...

Embodiment 3

[0047] After the fermentation broth is filtered, most of the protein is removed through an ultrafiltration membrane with a molecular weight cut-off of 40,000 Daltons, and the supernatant is diluted to a concentration of sialic acid of about 15g / L, and then pumped into a continuous ion exchange machine filled with anion exchange resin. system. A continuous ion exchange system consisting of 14 anion exchange columns is adopted, 4 in the adsorption area, 3 in the impurity washing area, 4 in the elution area, and 3 in the regeneration area. Each zone can be connected in series to increase the concentration of the target product effluent. Each ion exchange column is filled with 1.2Kg resin. The adsorption flow rate is 60ml / min, and the adsorption capacity is 0.244g / g wet resin; the washing liquid is deionized water, and the washing flow rate is 50ml / min. An ion-exchange column has no impurity to flow out); The eluent is the sodium chloride solution of 0.8mol / L, and the elution fl...

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Abstract

The invention discloses a method for continuously separating sialic acid, wherein sialic acid is prepared through microbial fermentation by taking N-acetyl glucosamine and sodium pyruvate as substrates; fermentation liquor is processed through filtration and ultrafiltration so as to obtain sialic acid clear liquid; sialic acid clear liquid is pumped in a continuous ion exchange system provided with OH-type anion exchange resin for adsorption; deionized water is adopted for leaching; a sodium chloride solution is adopted for elution; a sodium hydroxide solution is adopted for resin regeneration; eluent containing sialic acid is collected, is leached through an ethanol water solution, and then is added with ethyl acetate for dilution crystallization; and crystals are dried under reduced pressure, so that pure sialic acid is obtained. The separation and purification method is simple, convenient and feasible, achieves good effect and low running cost, and can implement the separation from kilogram class to ton class according to the separation amount; and in addition, products that are obtained by adopting the method gain excellent results on yield coefficient and product quality, and the product quality is ensured..

Description

technical field [0001] The invention relates to a method for continuously separating sialic acid, which belongs to the technical field of separation and purification of biological products. Background technique [0002] Sialic acid (Sialic Acid, SA) is a general term for nine-carbon sugar neuraminic acid acylate, which widely exists in a variety of biological tissues. The sialic acid found in most mammalian tissues is mainly N-acetylneuraminic acid, so N-acetylneuraminic acid is usually called sialic acid. Sialic acid is widely distributed in nature. It has been found that sialic acid exists in many organisms. It is usually located at the key position of the sugar part of the outermost layer of the cell membrane and the secreted sugar complex (glycolipid, glycoprotein and lipopolysaccharide). An important material basis for complex structure and function diversification. [0003] There are two types of biological functions of sialic acid, that is, the receptor function tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H7/033C07H1/06
Inventor 应汉杰王裴吴菁岚陈勇陈晓春谢婧婧柏建新胡亚南
Owner NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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